Identification and functional analysis of novel bioactive peptides that regulate energy metabolism and food intake

• Project/Area Number: 23791066
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: YOSHIDA Morikatsu 独立行政法人国立循環器病研究センター, 研究所, 研究員 (70393212)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 生理活性ペプチド / オーファン受容体 / 摂食・エネルギー代謝調節 / 神経ペプチド / ペプチドホルモン / オーファンGPCR / 摂食行動 / エネルギー代謝調節 / エネルギー代謝

Research Abstract

We performed a search for newly bioactive peptides acting on orphan receptors expressed in the hypothalamus which controls the energy metabolism and feeding behavior. For screening candidate ligands of these receptors, we developed novel activity detection system which is determined by microscopic morphological changes of cells (CellKey assay). Candidate ligands in tissue extracts were screened against cell lines expressing target orphan receptors by using this system. In cells expressing a certain orphan receptor, we detected the significant increase of impedance, which was the target receptor specific. We are now proceeding to purify the active peptide from this extract.

Research Products

• [Journal Article] Isolation of the bioactive peptides CCHamide-1 and CCHamide-2 from Drosophila and their putative role in appetite regulation as ligands for G protein-coupled receptors. (2012)
  • Author(s): Ida, T., Takahashi, T., Tominaga, H., Sato, T., Sano, H., Kume, K., Ozaki, M., Hiraguchi, T., Shiotani, H., Terajima, S., Nakamura, Y., Mori, K., Yoshida, M., Kato, J., Murakami, N., Miyazato, M., Kangawa, K., Kojima, M.
  • Journal Title: Front Endocrinol (Lausanne). Volume: 3 Pages: 177-177
  • Peer Reviewed
• [Journal Article] Isolation of the bioactive peptides CCHamide-1 and CCHamide-2 from Drosophila and their putative role in appetite regulation as ligands for G protein-coupled receptors (2012)
  • Author(s): Ida T, Takahashi T, Tominaga H, Sato T, Sano H, Kume K, Ozaki M, Hiraguchi T, Shiotani H, Terajima S, Nakamura Y, Mori K, Yoshida M, Kato J, Murakami N, Miyazato M, Kangawa K, Kojima M
  • Journal Title: Front Endocrinol (Lausanne) Volume: 3 Pages: 177-177
  • DOI: 10.3389/fendo.2012.00177
  • Peer Reviewed
• [Journal Article] Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila. (2011)
  • Author(s): Ida, T., Takahashi, T., Tominaga, H., Sato, T., Kume, K., Ozaki, M., Hiraguchi, T., Maeda, T., Shiotani, H., Terajima, S., Sano, H., Mori, K., Yoshida, M., Miyazato, M., Kato, J., Murakami, N., Kangawa, K., Kojima, M.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 410(4) Pages: 872-7
  • Peer Reviewed
• [Journal Article] Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila. (2011)
  • Author(s): Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Ozaki M, Hiraguchi T, Maeda T, Shiotani H, Terajima S, Sano H, Mori K, Yoshida M, Miyazato M, Kato J, Murakami N, Kangawa K, Kojima M.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 410 (4) Pages: 872-877
  • Peer Reviewed
• [Book] Orphan GPCRs and methods for identifying their ligands. Methods Enzymol. (2012)
  • Author(s): oshida, M., Miyazato, M., Kangawa, K.
• [Book] Methods in Enzymology (2012)
  • Author(s): Yoshida, M., et al.
  • Total Pages: 452
  • Publisher: Elsevier
• [Remarks] (招聘講義) 抗体の精製・抗体アフィニティの基礎から応用まで,宮崎大学IR推進機構テクニカルセミナー(2012.8.28～30宮崎)