Demonstration of Hes-1oscillation in progenitors and analysis of its effect on differentiation in hematopoiesis

Research Project

Project/Area Number	23791070
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Hematology
Research Institution	University of Tsukuba
Principal Investigator	YOKOYAMA Yasuhisa 筑波大学, 医学医療系, 講師 (70512820)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	血液腫瘍学 / Hes-1 / 白血病 / 発現振動 / Hes1 / oscillation / leukemia / hematopoiesis
Research Abstract	We demonstrated ultradian oscillation of Hes-1 expression in single hematopoietic progenitor cells separated from murine fetal liver. Hematopoietic progenitors introduced with MLL-AF9 fusion gene cause leukemia in in vivo model. When progenitors derived from Hes-1 knockout mice were used, the Hes-1-knockout cells with MLL-AF9 caused earlier death than wild-type cells, which indicated that Hes-1 works as tumor suppressor gene in MLL-AF9 leukemia.

Report

(3 results)