Role of histone acetyltransferase, Hbo1, in hematopoiesis

• Project/Area Number: 23791071
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Chiba University
• Principal Investigator: MIYAGI Satoru 千葉大学, 大学院・医学研究院, 助教 (20400997)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: T細胞分化 / 造血幹細胞 / クロマチン / 転写制御 / 遺伝子発現制御 / 胸腺細胞分化

Research Abstract

Recent progress has clearly demonstrated the significance of transcriptional regulation by histone acetyltransferase (HAT)s in the hematopoietic system. Our previous study showed that Hbol-Brdl HAT complex is the major H3K14 HAT required for transcriptional activation of erythroid developmental regulator genes and is dispensable for erythroid differentiation. In this study, we show that Hbol-Brdl HAT complex also has an important role in the induction of CD8 expression and thereby the T cell differentiation.

Research Products

• [Journal Article] Role of SOX17 in hematopoietic development from human embryonic stem cells (2013)
  • Author(s): Nakajima-Takagi, Y., Osawa, M., Oshima, M., Takagi, H., Miyagi, S., Endoh, M., Endo, T.A., Takayama, N., Eto, K., Toyoda, T., Koseki, H., Nakauchi, H. and Iwama, A
  • Journal Title: Blood Volume: 121 Pages: 447-458
  • Peer Reviewed
• [Journal Article] Role of SOX17 in hematopoietic development from human embryonic stem cells. (2013)
  • Author(s): Nakajima-Takagi Y, Osawa M, Oshima M, Takagi H, Miyagi S, Endoh M, Endo TA, Takayama N, Eto K, Toyoda T, Koseki H, Nakauchi H, Iwama A.
  • Journal Title: Blood. Volume: 121 Issue: 3 Pages: 447-58
  • DOI: 10.1182/blood-2012-05-431403
  • Peer Reviewed
• [Journal Article] Bmil confers resistance to oxidative stress on hematopoietic stem cells. (2012)
  • Author(s): Nakamura S, Oshima M, Yuan J, Saray a A, Miyagi S, Konuma T, Yamazaki S, Osawa M, Nakauchi H, Koseki H, Iwama A.
  • Journal Title: PLoS One Volume: 7(5) Issue: 5 Pages: e36209-e36209
  • DOI: 10.1371/journal.pone.0036209
  • Peer Reviewed
• [Journal Article] 3-Deazaneplanocin A is a promising therapeutic agent for the eradication of tumor-initiating hepatocellular carcinoma cells (2012)
  • Author(s): Chiba T, Suzuki E, Negishi M, Saraya A, Miyagi S, Konuma T, Tanaka S, Tada M, Kanai F, Imazeki F, Iwama A, Yokosuka O
  • Journal Title: International Journal of Cancer Volume: 130(11) Issue: 11 Pages: 2557-2567
  • DOI: 10.1002/ijc.26264
  • Peer Reviewed
• [Journal Article] Aldehyde dehydrogenase 1 is associated with recurrence-free survival but not stem cell-like properties in hepatocellular carcinoma (2012)
  • Author(s): Suzuki, E., Chiba, T., Zen, Y., Miyagi, S., Tada, M., Kanai, F., Imazeki, F., Miyazaki, M., Iwama, A. and Yokosuka, O
  • Journal Title: Hepatol Res Volume: 42 Pages: 1100-1111
  • Peer Reviewed
• [Journal Article] Ezh2 augments leukemogenicity by reinforcing differentiation blockage in acute myeloid leukemia (2012)
  • Author(s): Tanaka, S., Miyagi, S., Sashida, G., Chiba, T., Yuan, J., Mochizuki-Kashio, M., Suzuki, Y., Sugano, S., Nakaseko, C., Yoko te, K., Koseki, H. and Iwama, A
  • Journal Title: Blood Volume: 120 Pages: 1107-1117
  • Peer Reviewed
• [Journal Article] Emx2 is a dose-dependent negative regulator of Sox2 telencephalic enhancers. (2012)
  • Author(s): Mariani J, Favaro R, Lancini C, Vaccari G, Ferri ALM, Bertolini J, Tonoli D, Latorre E, Caccia R, Ronchi A, Ottolenghi S, Miyagi S, Okuda A, Zappavigna V, Nicolis SK.
  • Journal Title: Nucleic Acids Research Volume: 40 Issue: 14 Pages: 6461-6476
  • DOI: 10.1093/nar/gks295
  • Peer Reviewed
• [Journal Article] Lethal myelofibrosis induced by Bmil-deficient hematopoietic cells unveils a tumor suppressor function of the polycomb group genes (2012)
  • Author(s): Oguro H
  • Journal Title: J Exp Med Volume: 209 Issue: 3 Pages: 445-454
  • DOI: 10.1084/jem.20111709
  • Peer Reviewed
• [Journal Article] Aldehyde dehydrogenase 1 is associated with recurrence-free survival but not stem cell-like properties in hepatocellular carcinoma. (2012)
  • Author(s): Suzuki E, et al.
  • Journal Title: Hepatol Res Volume: 42 Pages: 1100-1111
  • Peer Reviewed
• [Journal Article] Ezh2 augments leukemogenecity by reinforcing differentiation blockage in acute myeloid leukemia (2012)
  • Author(s): Tanaka S
  • Journal Title: Blood Volume: 120 Issue: 5 Pages: 1107-17
  • DOI: 10.1182/blood-2011-11-394932
  • Peer Reviewed
• [Journal Article] Direct activation of Stat5 by ETV6-Lyn fusion protein promotes induction of myeloproliferative neoplasm with myelofibrosis (2011)
  • Author(s): Takeda, et al.
  • Journal Title: British Journal of Haematology Volume: 153 Issue: 5 Pages: 2874-2903
  • DOI: 10.1111/j.1365-2141.2011.08663.x
  • Peer Reviewed
• [Journal Article] Forced expression of the histone demethylase Fbxl10 maintains self-renewing hematopoietic stem cells (2011)
  • Author(s): Konuma T, et al
  • Journal Title: Experimental Hematology Volume: 39(6) Issue: 6 Pages: 697-709
  • DOI: 10.1016/j.exphem.2011.03.008
  • Peer Reviewed
• [Journal Article] The Hbo1-Brd1/Brpf2 complex is responsible for global acetylation of H3K14 and required for fetal liver erythropoiesis (2011)
  • Author(s): Mishima Y
  • Journal Title: Blood Volume: 118 Issue: 9 Pages: 2443-2453
  • DOI: 10.1182/blood-2011-01-331892
  • Peer Reviewed
• [Journal Article] Dependency on the polycomb gene Ezh2 distinguishes fetal from adult hematopoietic stem cells (2011)
  • Author(s): Mochizuki-Kashio M, Mishima Y, Miyagi S, Negishi M, Saraya A, Konuma T, Shinga J, Koseki H, Iwama A
  • Journal Title: Blood Volume: 118(25) Issue: 25 Pages: 6553-6561
  • DOI: 10.1182/blood-2011-03-340554
  • Peer Reviewed
• [Journal Article] The Hbo1-Brd1/Brpf2 complex is responsible (2011)
  • Author(s): Mishima Y
  • Journal Title: Blood Volume: 118 Pages: 2443-2453
  • Peer Reviewed