| Project/Area Number |
23791075
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
YAMAMOTO Ryo 東京大学, 医科学研究所, 特任研究員 (00581191)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 造血幹細胞 / 自己複製能 / 多分化能 / microRNA / 血液前駆細胞 / 細胞系譜決定 / 自己複製 |
|---|
| Research Abstract |
Hematopoietic stem cells (HSCs, CD34-KSL) have self-renewal activity and multipotency. CD150+ cells in CD34-KSL fraction have long-term and myeloid-biased repopulating activity. We have identified microRNAs specific to CD150+CD34-KSL fraction and transplanted microRNA-transduced CD34-KSLs into lethally-irradiated mice. However, we could not detect microRNAs that expanded self-renewal activity. This is, in part, due to HSC heterogeneity because candidate microRNAs have been reported to be expressed in megakaryocytes. To assess this, we performed single-cell transplantation of bone marrows from mice in which all mature blood cells express fluorescent protein. We detected myeloid-restricted repopulating cells in addition to full-lineage HSC in HSC fraction, suggesting that some of candidate microRNAs have a role in lineage commitment rather than self-renewal.
|
