Elucidation of the localization of hematopoietic stem cells and their niche using a novel cell surface marker, Plxdc2
Project/Area Number |
23791083
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | Saga University |
Principal Investigator |
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 移植・再生医療 / 発生・分化 / 造血幹細胞 / ニッチ / Plxdc2 |
Research Abstract |
We performed a stringent comparative gene expression profiling analysis to find genes preferentially expressed in the HSC population, and identified a total of 63 genes that are highly expressed in HSC among various hematopoietic cell population.In order to find HSC-specific marker we focused on genes encoding cell surface protein, and found that plexin domain containing 2 (Plxdc2) is highly expressed in CD34-c-Kit+Sca-1+Lineage-(CD34-KSL) HSC population using Plxdc2::GFP knock-in mice. Only 0.2% of whole bone marrow cells were Plxdc2+. Furthermore, Plxdc2+CD150+fraction represented only 0.01%±0.002% in whole bone marrow, suggesting that the simple combination of Plxdc2 and CD150 significantly increased HSC purity.
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Report
(3 results)
Research Products
(53 results)
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[Journal Article] Development of lymphoproliferative diseases by hypoxia inducible factor-1alpha is associated with prolonged lymphocyte survival.2013
Author(s)
Sueoka E, Sueoka-Aragane N, Sato A, Ide M, Nakamura H, Sotomaru Y, Taya C, Yonekawa H, Kitagawa T, Kubota Y, Kimura S, Nakachi K, Tanimoto K
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Journal Title
PLoS One
Volume: 8
Issue: 4
Pages: e57833-e57833
DOI
Related Report
Peer Reviewed
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[Journal Article] Successful reduced-intensity umbilical cord blood transplant for fulminant hemophagocytic syndrome in an adult with pre-existing rheumatoid arthritis and autoimmune hemolytic anemia.2012
Author(s)
Itamura H, Fukushima N, Kondo S, Urata C, Tanaka-Yoshimura M, Yokoo M, Ide M, Hisatomi T, Kubota Y, Sueoka E, Ichinohe T, Kimura S
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Journal Title
Leuk Lymphoma
Volume: 53
Issue: 11
Pages: 2307-2309
DOI
Related Report
Peer Reviewed
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[Presentation] The role of hypoxia inducible factors on maintenance of hematopoietic stem cells and leukemogenesis2012
Author(s)
Sueoka E, Ide M, Sueoka-Aragane N, Sato A, Nakamura H, Sotomaru Y, Taya C, Yonekawa H, Nakachi K, Kubota Y, Kimura S, Tanimoto K
Organizer
2012 Tumor Microenvironment GCRC International Symposium.
Place of Presentation
Seoul
Related Report
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[Presentation] Antiproliferative Effect of 2-Hydroxypropyl-ss-Cyclodextrin (HP-ss-CyD) Against Chronic Myeloid Leukemia In Vitro and In Vivo2012
Author(s)
Yokoo M, Kubota Y, Mochinaga S, Maeda A, Ichinohe T, Fujito H, Higashi T, Motoyama K, Arima H, Irie T, Kimura S
Organizer
The 54th Annual Meeting of the American Society of Hematology
Place of Presentation
Atlanta, USA
Related Report
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[Presentation] Concurrent development of invasive cryptococcosis and mature B-cell neoplasm in an HIV-negative young adult2012
Author(s)
Itamura H, Hisatomi T, Yoshimura M, Yokoo M, Ide M, Kubota Y, Fukushima N, Yoshihara M, Nagata M, Ichinohe T, Ohshima K, Aoki Y, Sueoka E, Kimura S
Organizer
第74回日本血液学会学術集会
Place of Presentation
京都
Related Report
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[Presentation] Successful reduced-intensity umbilical cord blood trans plantation for adult refractory hemophagocytic syndrome withanti-HLA antigen.2012
Author(s)
Itamura H,Fukushima N,Kondo S,Urata C,Yoshimura M,Yokoo M,Ide M,Hisatomi T, Kubota Y, Sueoka E, Ichinohe T, Kimura S.
Organizer
第34回日本造血細胞移植学会学術集会
Place of Presentation
大阪
Related Report
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[Presentation] Antiproliferative Effect of 2-Hydroxypropyl-β-Cyclodextrin (HP-β-CyD) Against Chronic Myeloid Leukemia In Vitro and In Vivo.2012
Author(s)
Yokoo M, Kubota Y, Mochinaga S, Maeda A, Ichinohe T, Fujito H, Higashi T, Motoyama K, Arima H, Irie T, Kimura S.
Organizer
The 54th Annual Meeting of the American Society of Hematology
Place of Presentation
Atlanta, USA
Related Report
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[Presentation] 赤芽球癆を合併したGood症候群2011
Author(s)
木村亜由美, 吉村麻里子, 板村英和, 久富崇, 久保田寧, 福島伯泰, 一戸辰夫, 末岡榮三朗, 木村晋也
Organizer
第295回日本内科学会九州地方会
Place of Presentation
佐賀
Year and Date
2011-11-20
Related Report
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[Presentation] 当院における濾胞型リンパ腫82例の臨床成績の検討2011
Author(s)
北村浩晃, 吉村麻里子, 福島伯泰, 板村英和, 浦田千紗子, 横尾眞子, 出勝, 久富崇, 久保田寧, 一戸辰夫, 末岡榮三朗, 木村晋也
Organizer
第51回日本リンパ網内系学会総会
Place of Presentation
福岡
Year and Date
2011-07-01
Related Report
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[Presentation] Relation between CNS involvement and involved extranodal site at presentation in DLBL2011
Author(s)
Itamura H, Fukushima N, Urata C, Yoshimura M, Yokoo M, Ide M, Hisatomi T, Kubota Y, Ichinohe T, Sueoka E, Kimura S.
Organizer
第73回日本血液学会学術集会
Place of Presentation
名古屋
Related Report
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[Presentation] Hemostatic abnormality in patients with adult T-cell leukemia-lymphoma2011
Author(s)
Kubota Y, Sano M, Kubota M, Itamura H, Urata C, Yoshimura M, Hisatomi T, Fukushima N, Sueoka E, Ichinohe T, Kimura S
Organizer
第73回日本血液学会学術集会
Place of Presentation
名古屋
Related Report
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[Presentation] Relation between CNS involvement and involved extranodal site at presentation in DLBL2011
Author(s)
Itamura H, Fukushima N, Urata C, Yoshimura M, Yokoo M, Ide M, Hisatomi T, Kubota Y, Ichinohe T, Sueoka E, Kimura S.
Organizer
第73回日本血液学会学術集会
Place of Presentation
名古屋市
Related Report
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[Presentation] Successful treatment with dasatinib for CML-BC patient having extramedullary tumor2011
Author(s)
Itoh Y, Itamura H, Urata C, Yoshimura M, Yokoo M, Ide M, Hisatomi T, Kubota Y, Fukushima N, Ichinohe T, Sueoka E, Kimura S.
Organizer
第73回日本血液学会学術集会
Place of Presentation
名古屋市
Related Report
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[Presentation] Hemostatic abnormality in patients with adult T-cell leukemia-lymphoma2011
Author(s)
Kubota Y, Sano M, Kubota M, Itamura H, Urata C, Yoshimura M, Hisatomi T, Fukushima N, Sueoka E, Ichinohe T, Kimura S.
Organizer
第73回日本血液学会学術集会
Place of Presentation
名古屋市
Related Report
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