Improvement of serological diagnosis of leptospirosis and its epidemiological dynamics

• Project/Area Number: 23791130
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Infectious disease medicine
• Research Institution: Osaka City University (2012-2013); Hokkaido University (2011)
• Principal Investigator: RIE ISOZUMI 大阪市立大学, 医学(系)研究科(研究院), 講師 (30550355)
• Research Collaborator: ARIKAWA Jiro 北海道大学, 大学院医学研究科, 教授 (10142704) ; YOSHIMATSU Kumiko 北海道大学, 大学院医学研究科, 准教授 (90220722) ; SHIMIZU Kenta 北海道大学, 大学院医学研究科, 助教 (20466840)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: レプトスピラ症 / 血清学的診断 / ELISA / 疫学動態 / 熱帯地域 / ELISA法 / 早期診断

Research Abstract

Leptospirosis is wide spread disease especially in tropical developing countries, which cause symptom in human often severe to lethal. Early diagnosis and early treatment is quite important to avoid bad outcome, but it is difficult to diagnose at early stage of disease by current diagnose methods whose sensitivity and specificity are also insufficient to do complete diagnosis. With the diagnostic problem, we do not understand accurate epidemic status of leptospirosis in tropical developing countries. In this study, we developed a new diagnostic method based on ELISA with recombinant antigens (LipL32 and LigA). We tested samples from epidemic area (Thailand, Sri Lanka and Vietnam) with this method. We also investigated wild rats in Hanoi which are important vector of leptospirosis.

Research Products

• [Journal Article] Characteristic age distribution of Plasmodium vivax infections after malaria elimination on Aneityum Island (2014)
  • Author(s): Kaneko, A.; Chaves, L. F.; Taleo, G.; Kalkoa, M.; Isozumi, R.; Wickremasinghe, R.; Perlmann, H.; Takeo, S.; Tsuboi, T.; Tachibana, S.; Kimura, M.; Bjorkman, A.; Troye-Blomberg, M.; Tanabe, K.; Drakeley, C.
  • Journal Title: Vanuatu. Infect Immun Volume: 82(1) Pages: 243-52
  • Peer Reviewed
• [Journal Article] Characteristic Age Distribution of Plasmodium vivax Infections after Malaria Elimination on Aneityum Island, Vanuatu. (2014)
  • Author(s): Kaneko A, Chaves LF, Taleo G, Kalkoa M, Isozumi R, Wickremasinghe R, et al.
  • Journal Title: Infect Immun Volume: 82 Pages: 243-252
  • Peer Reviewed
• [Journal Article] A survey of rodent-borne pathogens carried by wild Rattus spp. in Northern Vietnam (2013)
  • Author(s): Koma, T.; Yoshimatsu, K.; Yasuda, S. P.; Li, T.; Amada, T.; Shimizu, K.; Isozumi, R.; Mai, L. T.; Hoa, N. T.; Nguyen, V.; Yamashiro, T.; Hasebe, F.; Arikawa, J.
  • Journal Title: Epidemiol Infect Volume: 141(9) Pages: 1876-84
  • Peer Reviewed
• [Journal Article] Cross-reactivity of secondary antibodies against African rodents and application for sero-surveillance (2013)
  • Author(s): Nakamura, I.; Hang'ombe, B. M.; Sawa, H.; Kobayashi, S.; Orba, Y.; Ishii, A.; Thomas, Y.; Isozumi, R.; Yoshimatsu, K.; Mweene, A. S.; Takada, A.; Sugimoto, C.; Arikawa, J.
  • Journal Title: J Vet Med Sci Volume: 75(6) Pages: 819-25
  • NAID: 130003362128
  • Peer Reviewed
• [Journal Article] A survey of rodent-borne pathogens carried by wild Rattus spp. in Northern Vietnam. (2013)
  • Author(s): Koma T, Yoshimatsu K, Yasuda SP, Li T, Amada T, Shimizu K, Isozumi R et al.
  • Journal Title: Epidemiol Infect. Volume: 141 Pages: 1876-1884
  • Peer Reviewed
• [Journal Article] A survey of rodent-borne pathogens carried by wild Rattus spp. in Northern Vietnam (2013)
  • Author(s): Koma, T., K. Yoshimatsu, S. P. Yasuda, T. Li, T. Amada, K. Shimizu, R. Isozumi, L. T. Mai, N. T. Hoa, V. Nguyen, T. Yamashiro, F. Hasebe, and J. Arikawa.
  • Journal Title: Epidemiol Infect Volume: Epub ahead of pri
  • Peer Reviewed
• [Journal Article] Cross-Reactivity of Secondary Antibodies against African Rodents and Application for Sero-Surveillance (2013)
  • Author(s): Nakamura, I., B. M. Hang'ombe, H. Sawa, S. Kobayashi, Y. Orba, A. Ishii, Y. Thomas, R. Isozumi, K. Yoshimatsu, A. S. Mweene, A. Takada, C. Sugimoto, and J. Arikawa
  • Journal Title: J Vet Med Sci Volume: Epub ahead of pri
  • NAID: 130003362128
  • Peer Reviewed
• [Journal Article] from environment, Vietnam (2012)
  • Author(s): Isozumi, R.; Yoshimatsu, K.; Yamashiro, T.; Hasebe, F.; Nguyen, B. M.; Ngo, T. C.; Yasuda, S. P.; Koma, T.; Shimizu, K.; Arikawa, J.
  • Journal Title: Emerg Infect Dis Volume: 18(8) Pages: 1383-5
  • Peer Reviewed
• [Journal Article] Application of truncated nucleocapsid protein (N) for serotyping ELISA of murinae-associated hantavirus infection in rats (2012)
  • Author(s): Yasuda, S. P.; Yoshimatsu, K.; Koma, T.; Shimizu, K.; Endo, R.; Isozumi, R.; Arikawa, J.
  • Journal Title: J Vet Med Sci Volume: 74(2) Pages: 215-9
  • NAID: 130001033057
  • Peer Reviewed
• [Journal Article] bla(NDM-1)-positive Klebsiella pneumoniae from Environment, Vietnam. (2012)
  • Author(s): Isozumi, R., K. Yoshimatsu, T. Yamashiro, F. Hasebe, B. M. Nguyen, T. C. Ngo, S. P. Yasuda, T. Koma, K. Shimizu, and J. Arikawa.
  • Journal Title: Emerg Infect Dis Volume: 18 Issue: 8 Pages: 1383-1385
  • DOI: 10.3201/eid1808.111816
  • NAID: 120006985570
  • Peer Reviewed
• [Journal Article] Application of truncated nucleocapsid protein (N) for serotyping ELISA of murinae-associated hantavirus infection in rats. (2012)
  • Author(s): Yasuda, S. P., K. Yoshimatsu, T. Koma, K. Shimizu, R. Endo, R. Isozumi, and J. Arikawa.
  • Journal Title: J Vet Med Sci Volume: 74 Pages: 215-219
  • NAID: 130001033057
  • Peer Reviewed
• [Journal Article] Plasmodium falciparum : differential selection of drug resistance alleles in contiguous urban and per -urban areas of Brazzaville, Republic of Congo (2011)
  • Author(s): Tsumori, Y.; Ndounga, M.; Sunahara, T.; Hayashida, N.; Inoue, M.; Nakazawa, S.; Casimiro, P.; Isozumi, R.; Uemura, H.; Tanabe, K.; Kaneko, O.; Culleton, R.
  • Journal Title: PLoS One Volume: 6(8)
  • Peer Reviewed
• [Presentation] スリランカ産Rattus rattusおよびBandicota bengalensisの系統学的位置 (2012)
  • Author(s): 安田俊平、Chandika D. Gamage、小泉信夫、西尾佐奈恵、五十棲理恵、清水健太、駒貴明、天田貴子、鈴木仁、吉松組子、有川二郎
  • Organizer: 日本哺乳類学会2012年度大会
  • Place of Presentation: 麻布大学(神奈川県)
• [Presentation] Seroprevalence of anti-leptospira antibodies among patients with acute febrile illness with renal dysfunction in spite of negative result with several leptospira tests in Thailand (2011)
  • Author(s): Rie Isozumi, Kumiko Yoshimatsu, Sirima Pattamadilok, Sanit Kumperasart, Jiro Arikawa
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-09
• [Presentation] Seroprevalence of anti-leptospira antibodies among patients with acute febrile illness with renal dysfunction in spite of negative result with several leptospira tests in Thailand (2011)
  • Author(s): Rie Isozumi, Kumiko Yoshimatsu, Sirima Pattamadilok, Sanit Kumperasart, Jiro Arikawa
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター（北海道）