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A study of Reye-like syndrome associated with emetic toxin of bacillus cereus

Research Project

Project/Area Number 23791176
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pediatrics
Research InstitutionShimane University

Principal Investigator

KOBAYASHI Hironori  島根大学, 医学部, 助教 (70397868)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords脂肪酸代謝障害 / ミトコンドリア / セレウス菌 / セレウリド / タンデムマス / アシルカルニチン / ライ様症候群
Research Abstract

This study was aimed to investigate the effect on the fatty acid metabolism of the patients with Bacillus cereus infection by an acylcarnitine analysis using tandem mass spectrometry, and to investigate effect of cereulide, emetic toxin from B. cereus, on fatty acid metabolism using in vitro acylcarnitine probe (IVP) assay of cultured fibroblasts. The acylcarnitie-profile of two patients with B. cereus infection revealed to be non-specific elevation of long-chain acylcarnitines. The result of IVP assay revealed that cereulide could inhibit fatty acid metabolism profiles fatty acid metabolism, resulting the similar acylcarnitine-profile to glutaric aciduria type 2. In addition, cereulide impaired fatty acid oxidation in concentration -dependent, the profile of acylcarnitines of IVP assay suggested toxic effect of cereulide on fatty acid oxidation was actualized from concentration of 50-100 ng/ml.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (14 results)

All 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (9 results)

  • [Journal Article] Intracellular in vitro probe acylcarnitine assay for identifying deficiencies ofcarnitine transporter and carnitine palmitoyltransferase-12013

    • Author(s)
      Purevsuren J
    • Journal Title

      Anal Bioanal Chem

      Volume: 405 Issue: 4 Pages: 1345-1351

    • DOI

      10.1007/s00216-012-6532-3

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bezafibrate can be a new treatment option for mitochondrial fatty acid oxidation disorders: Evaluation by in vitro probe acylcarnitine assay2012

    • Author(s)
      Yamaguchi S, Li H, Purevsuren J, Yamada K, Furui M, Takahashi T, Mushimoto Y, Kobayashi H, Hasegawa Y, Taketani T, Fukao T, Fukuda S
    • Journal Title

      Molecular Genetics and Metabolism

      Volume: 107 Pages: 87-91

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinical and molecular aspects of Japanese children with medium chain acyl-CoA dehydrogenase deficiency2012

    • Author(s)
      Purevsuren J, Hasegawa Y, Fukuda S, Kobayashi H, Mushimoto Y, Yamada K, Takahashi T, Fukao T, Yamaguchi S
    • Journal Title

      Molecular Genetics and Metabolism

      Volume: 107 Pages: 237-240

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Retrospective review of Japanese sudden unexpected death in infancy: The importance of metabolic autopsy and expanded newborn screening2011

    • Author(s)
      Yamamoto T, Tanaka H, Kobayashi H, Okamura K, Tanaka T, Emoto Y, Sugimoto K, Nakatome M, Sakai N, Kuroki H, Yamaguchi S, Matoba R
    • Journal Title

      Molecular Genetics and Metabolism

      Volume: 102(4) Pages: 399-406

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A patient with mitochondrial trifunctional protein deficiency due to the mutations in the HADHB gene showed recurrent myalgia since early childhood and was diagnosed in adolescence2011

    • Author(s)
      Yagi M, Lee T, Awano H, Tsuji M, Tajima G, Kobayashi H, Hasegawa Y, Yamaguchi S, Takeshima Y, Matsuo M
    • Journal Title

      Molecular Genetics and Metabolism

      Volume: 104(4) Pages: 556-559

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 新生児発症型グルタル酸尿症 2 型男児例に対する Bezafibrate 治療の経験2012

    • Author(s)
      小林弘典, 和田泰格, 鈴木絵理, 山田健治, 高橋知男, プレブスレン ジャミヤン, 長谷川有紀, 長谷川行洋, 山口清次
    • Organizer
      第54回日本先天代謝異常学会
    • Place of Presentation
      (岐阜市)じゅうろくプラザ
    • Related Report
      2012 Final Research Report
  • [Presentation] ピボキシル基を含む抗菌薬によりカルニチン欠乏症をきたした 24 例の臨床像2012

    • Author(s)
      小林弘典, 高橋知男, 山田健治, 長谷川有紀, 山口清次
    • Organizer
      第64回中国四国小児科学会
    • Place of Presentation
      (香川)香川大学
    • Related Report
      2012 Final Research Report
  • [Presentation] 新生児発症型グルタル酸尿症 2 型に対するベザフィブラートの有効性の検討: In vitro probe acylcarnitine assay の結果との関連性2012

    • Author(s)
      山 田 健 治 , 小林弘典 , Jamiyan Purevsuren, 高橋知男, 長谷川有紀, 山口清次
    • Organizer
      第37回日本医用マススペクトル学会年会
    • Place of Presentation
      (名古屋)愛知県産業労働センター
    • Related Report
      2012 Final Research Report
  • [Presentation] Acylcarnitine analysis by ESI-MS/MS with smaller amount of sample and rapid analytical time2012

    • Author(s)
      Kobayashi H, Mushimoto Y, Hasegawa Y, Yamada K, Purevsuren J, Takahashi T, Minohata T, Iida J, Yamaguchi S
    • Organizer
      19th International Mass Spectrometry Conference
    • Place of Presentation
      Kyoto International Conference Center, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Application of in-vitro probe acylcarnitine assay using tandem mass spectrometry for the evaluation of mitochondrial fatty acid oxidation2012

    • Author(s)
      Prevsuren J, Kobayashi H, Hasegawa Y, Yamada K, Takahashi T, Yamaguchi S
    • Organizer
      19th International Mass Spectrometry Conference
    • Place of Presentation
      Kyoto International Conference Center, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] セレウス菌食中毒による急性脳症はミトコンドリアβ酸化障害を介して起こる2012

    • Author(s)
      山口清次, Prevsuren J, 小林弘典, 長谷川有紀
    • Organizer
      第54回日本小児神経学会
    • Place of Presentation
      (札幌)ロイトン札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] Beneficial effect of bezafibrate on boy with the late-onset gluraric aciduria type 22012

    • Author(s)
      Kobayashi H, Yamada K, Prevsuren J, Mushimoto Y, Takahashi T, Hasegawa Y,Ito M, Yamaguchi S
    • Organizer
      The 2nd Asian Congress for Inherited Metabolic Diseases & The 12th Asian-European Workshop on Inborn Errors of Metabolism & The 12th Korean Congress of Inherited Metabolic Disease
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2012 Final Research Report
  • [Presentation] Emetic toxin of bacillus cereus that associates with reye-like syndrome severely inhibits mitochondrial fatty acid oxidation2011

    • Author(s)
      Purevsuren J, Hasegawa Y, Kobayashi H, Mushimoto Y, Yamada K, Okamoto A, Ohta M, Yamaguchi S
    • Organizer
      Annual Symposium of the Society for the Study of Inborn Errors of Matabolism
    • Place of Presentation
      Geneva, Switzerland
    • Related Report
      2012 Final Research Report
  • [Presentation] Emetic toxin of bacillus cereus that associates with reye-like syndrome severely inhibits mitochondrial fatty acid oxidation2011

    • Author(s)
      Purevsuren J, Hasegawa Y, Kobayashi H, Mushimoto Y, Yamada K, Okamoto A, Ohta M, Yamaguchi S
    • Organizer
      Annual Symposium of the Society for the Study of Inborn Errors of Matabolism
    • Place of Presentation
      International Conference Center Geneva, Switzerland
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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