| Project/Area Number |
23791176
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Shimane University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 脂肪酸代謝障害 / ミトコンドリア / セレウス菌 / セレウリド / タンデムマス / アシルカルニチン / ライ様症候群 |
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| Research Abstract |
This study was aimed to investigate the effect on the fatty acid metabolism of the patients with Bacillus cereus infection by an acylcarnitine analysis using tandem mass spectrometry, and to investigate effect of cereulide, emetic toxin from B. cereus, on fatty acid metabolism using in vitro acylcarnitine probe (IVP) assay of cultured fibroblasts. The acylcarnitie-profile of two patients with B. cereus infection revealed to be non-specific elevation of long-chain acylcarnitines. The result of IVP assay revealed that cereulide could inhibit fatty acid metabolism profiles fatty acid metabolism, resulting the similar acylcarnitine-profile to glutaric aciduria type 2. In addition, cereulide impaired fatty acid oxidation in concentration -dependent, the profile of acylcarnitines of IVP assay suggested toxic effect of cereulide on fatty acid oxidation was actualized from concentration of 50-100 ng/ml.
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