| Project/Area Number |
23791182
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Kochi University |
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Principal Investigator |
YAMAMOTO MASAKI 高知大学, 教育研究部医療学系, 助教 (20571037)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | GLP-1 / トロンボキサンA2 / α1アドレノセプター / Fos / 視床下部室傍核 / LC/MS/MS / α１アドレノセプター / 血中カテコラミン / αアドレノセプター / プロスタノイド / ノルアドレナリン |
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| Research Abstract |
In the present study, we used liquid chromatography ion trap tandem mass spectrometry (LC-ITMSn) to simultaneously quantify four prostanoids, thromboxane (Tx) B2, PGE2, PGD2 and 15-deoxy-delta12,14 (15d)-PGJ2 in PVN microdialysates from urethane-anesthetized rats. The quantification limits were estimated to be 0.05 ng/mL for TxB2, 0.025 ng/mL for PGE2, 0.1 ng/mL for PGD2, and 0.5 ng/mL for 15d-PGJ2. The RSD% obtained from all prostanoids was <15%, indicating an acceptable level of reproducibility. LC-ITMSn analysis of rat PVN microdialysates revealed that TxA2 may play an important role in adrenomedullary outflow evoked by centrally administered N-methyl-D-aspartate, corticotrophin-releasing factor and glucagon-like peptide-1. This is the first study to use LC-ITMSn to analyze prostanoid levels in rat PVN microdialysates. This LC-ITMSn method will be useful for investigating the potential involvement of prostanoids in brain function.
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