The internalization mechanism of Ureaplasma parvum in HeLa cells.
| Project/Area Number |
23791241
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Research Institute, Osaka Medical Center for Maternal and Child Health |
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Principal Investigator |
NISHIUMI Fumiko 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), その他部局等, 流動研究員 (60599596)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ウレアプラズマ / 絨毛膜羊膜炎 / エンドサイトーシス / エンドソーム |
|---|
| Research Abstract |
Ureaplasma parvum (U. parvum) is a causative of preterm delivery and colonizes human urogenital tract. In order to clarify the intracellular viability of U. paravum in the host cells, U. parvum was challenged to HeLa cells. U. parvum accumulated in the perinuclear region of the HeLa cells within 48 h after infection. Internalization of U. parvum into the HeLa cells was suppressed both by the clathrin-dependent endocytosis inhibitor chlorpromazine hydrochloride (CPZ) and the siRNA. These experiments indicated that U. parvum was internalized into HeLa cells via clathrin-mediated endocytosis. U. parvum were colocalized with early to late endosome markers. The increased production of ROS was observed in the U. parvum infected cells, which lead to structural changes in the mitochondrial cristae and swelling of mitochondria. Our findings raise the possibility that CPZ may have a potential role in the phylaxis of U. parvum infections.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] S1-1/RBM10 : Multiplicity and cooperativity of nuclear localization domains2013
Author(s)
Xiao S. J., Wang L. Y., Kimura, M., Kojima H., Kunimoto H., Nishiumi F., Yamamoto N., Nishio K., Fujimoto S., Kato T., Kitagawa S., Yamane H., Nakajima K., Inoue A.
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Journal Title
Biol. Cell
Volume: 105(4)
Pages: 162-174
Related Report
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[Journal Article] S1-1/RBM10: Multiplicity and cooperativity of nuclear localisation domains.2013
Author(s)
Xiao SJ, Wang LY, Kimura M, Kojima H, Kunimoto H, Nishiumi F, Yamamoto N, Nishio K, Fujimoto S, Kato T, Kitagawa S, Yamane H, Nakajima K, Inoue A.
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Journal Title
Biol Cell.
Volume: 105(4)
Pages: 162-174
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[Presentation] 非晶質ナノシリカの細胞膜への結合とナノ生殖毒性との関連2012
Author(s)
小野寺 章, 西海 史子, 古田 拓也, 中平 久美子, 石井 幸奈, 本間 安季, 太田 舞子, 諸澤瑛, 福井 健太郎, 米村 重信, 柳原 格, 堤 康央, 河合 裕一
Organizer
第85回日本生化学会大会
Place of Presentation
福岡
Related Report
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[Presentation] Virulence factors of Ureaplasma parvum.2011
Author(s)
Itaru Yanagihara, Kaoru Uchida, Kumiko Nakahira and Fumiko Nishiumi
Organizer
Joint Congress of The 5th Meeting of Asian Organization for Mycoplasmology. The 38th Meeting of the Japanese Society of Mycoplasmology.
Place of Presentation
Nagasaki
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