Control of spindle orientation in skin basal cells by c-Abland possible role of calcium signaling in spindle orientation

• Project/Area Number: 23791265
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kyoto University
• Principal Investigator: MATSUMURA Shigeru 京都大学, ウイルス研究所, 助教 (60523511)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞分裂軸 / 皮膚基底細胞 / ABL1 / 分裂軸方向制御 / 皮膚

Research Abstract

We performed a genome-scale RNA-mediated interference screen of human kinases using the HeLa cell system and identified ABL1 tyrosine kinase as a novel regulator for spindle orientation in mammalian cells. We find that ABL1 regulates spindle orientation not only in HeLa cells but also in mouse epidermis. Furthermore, we found that ABL1 regulates spindle orientation through LGN and NuMA, which form an evolutionarily conserved regulatory complex for spindle orientation.

Research Products

• [Journal Article] "ABL1 regulates spindle orientation in adherent cells and mammalian skin." (2012)
  • Author(s): Matsumura, S., Hamasaki, M., Yamamoto, T., Ebisuya, M., Sato, M., Nishida, E., and *Toyoshima, F.
  • Journal Title: Nat. Commun. Volume: 3 Issue: 1 Pages: 626-626
  • DOI: 10.1038/ncomms1634
  • Peer Reviewed
• [Journal Article] "ABL1 joins the cadre of spindle orientation machinery." (2012)
  • Author(s): Matsumura, S., and Toyoshima, F.
  • Journal Title: Cell Struct. Funct. Volume: 37 Pages: 81-87
  • NAID: 130004137581
  • Peer Reviewed
• [Journal Article] ABL1 Joins the Cadre of Spindle Orientation Machinery (2012)
  • Author(s): Matsumura, S., and Toyoshima, F.
  • Journal Title: Cell Structure and Function Volume: 37 Issue: 2 Pages: 81-87
  • DOI: 10.1247/csf.12011
  • NAID: 130004137581
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Presentation] Mitotic Plasma Membrane Domain Components Regulate Spindle Orientation (2012)
  • Author(s): Shigeru Matsumura, Fumiko Toyoshima
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡
• [Presentation] Talk "Spindle orientation machinery in mammalian cells" Shigeru Matsumura (2012)
  • Author(s): Shigeru Matsumura
  • Organizer: 19th East Asia Joint Symposium on Biomedical Research
  • Place of Presentation: Mokam Hall, Seoul National University Seoul, Korea
• [Presentation] 細胞分裂期における紡錘体の位置決めを制御するメカニズムの解析松村繁、豊島文子 (2012)
  • Author(s): 松村繁、豊島文子
  • Organizer: 第四回年会
  • Place of Presentation: 名古屋
• [Presentation] Spindle orientation machinery in mammalian cells (2012)
  • Author(s): Shigeru Matsumura
  • Organizer: 19th East Asia Joint Symposium on Biomedical Research
  • Place of Presentation: Seoul, Korea
• [Presentation] 細胞分裂期における紡錘体の位置決めを制御するメカニ ズムの解析 (2012)
  • Author(s): 松村繁、豊島文子
  • Organizer: 定量生物学の会 第四回年会
  • Place of Presentation: 名古屋
• [Presentation] Exciting Biologies Cellular development (2011)
  • Author(s): Shigeru Matsumura, Mayumi Hamasaki, Takuya Yamamoto, Miki Ebisuya, Mizuho Sato, Eisuke Nishida, Fumiko Toyoshima
  • Organizer: "Biology at the interface"
  • Place of Presentation: Kobe, Japan (oral, poster)
• [Presentation] ABL1 regulates spindle orientaion in adherent cells and mammalian skin (2011)
  • Author(s): Shigeru Matsumura, Mayumi Hamasaki,Takuya Yamamoto, Miki Ebisuya, Mizuho Sato, Eisuke Nishida, Fumiko Toyoshima "Stem Cell Biology"
  • Organizer: Cold Spring Harbor Laboratory Meeting
  • Place of Presentation: New York, U.S.A. (poster)
• [Presentation] ABL1による細胞分裂軸方向の制御機構 (2011)
  • Author(s): 松村繁、濱崎眞弓、山本拓也、戎家美紀、佐藤みずほ、西田栄介、豊島文子
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 札幌
• [Presentation] ABL1 regulates spindle orientaion in adherent cells and mammalian skin. (2011)
  • Author(s): Shigeru Matsumura, Mayumi Hamasaki, Takuya Yamamoto, Miki Ebisuya, Mizuho Sato, Eisuke Nishida, Fumiko Toyoshima
  • Organizer: Cold Spring Harbor Laboratory Meeting, "Stem Cell Biology"
  • Place of Presentation: New York, U.S.A.
• [Presentation] ABL1 regulates spindle orientaion in adherent cells and mammalian skin. (2011)
  • Author(s): Shigeru Matsumura, Mayumi Hamasaki, Takuya Yamamoto, Miki Ebisuya, Mizuho Sato, Eisuke Nishida, Fumiko Toyoshima
  • Organizer: Exciting Biologies Cellular development, "Biology at the interface"
  • Place of Presentation: Kobe, Japan
• [Book] ヒトと医学のステージへ拡大する細胞周期、 (2013)
  • Author(s): 松村繁、豊島文子、
  • Total Pages: 8
  • Publisher: 羊土社、
• [Book] ヒトと医学のステージへ拡大する細胞周期 (2013)
  • Author(s): 松村繁、豊島文子
  • Total Pages: 8
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.lif.kyoto-u.ac.jp/j/modules/research/content0028.html
• [Remarks] 京都新聞(1月18日 25面)、日刊工業新聞(1月19日 25面)および毎日新聞(1月18日 4面)に掲載されました。