Analysis of the effect of LED to skin compornent cells

• Project/Area Number: 23791273
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Ehime University
• Principal Investigator: MIYAWAKI Saori 愛媛大学, 医学部附属病院, 助教 (90467853)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 発光ダイオード / 創傷治癒 / 皮膚構成細胞 / 細胞増殖抑制 / 細胞増殖能 / 角化細胞 / 線維芽細胞 / 血管内細胞 / リンパ管内皮細胞 / 血管内皮細胞

Research Abstract

We conducted this study to establish the phototherapy for wound healing using a light emitting diode, and to clarify the mechanism.We have created a light-emitting diode irradiation equipment of three wavelengths. After irradiated respective wavelengths for the skin component cells, we examined cell proliferation and cytotoxicity .As a hypothesis, it was predicted skin component cells proliferate by exposure to a light emitting diode, it would show efficacy in wound healing. Contrary to expectation, we could not observed cell proliferation after irradiation all wavelengths lights respectivety.However,this study found thatskin component cells induced apoptosis in dose-dependent after irradiation of 415nm light emitting diode.