The effects of skin tumor microenvironment on dendritic cellmediated immune response

• Project/Area Number: 23791276
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kyushu University
• Principal Investigator: NAKAHARA Takeshi 九州大学, 九州大学病院, 講師 (40529848)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 樹状細胞 / 悪性黒色腫 / 腫瘍免疫

Research Abstract

We examined phenotypic analysis of tumor-infiltrating dendritic cell (TIDC) at different time points after tumor injection. Murine melanoma were infiltrated by many conventional DC especially at early time point. Other dendritic cell subsets like plasmacytoid DC and CD11c+, B220+, Gr-1-, NK1.1+ DC were also infiltrating into the tumor although their proportion was very low. TIDC were a little more mature than lymphoid resident DCs in dLNs.From these results, we found that there were much more TIDC than expected at early stage of tumor growth, and detailed analysis of functions of these TIDC might be able to lead the new approach for enhancement of tumor immunity.

Research Products

• [Journal Article] The prognostic value of a reverse transcriptase-PCR assay of sentinel lymph node biopsy for patients with cutaneous melanoma: a single-center analysis in Japan. (2012)
  • Author(s): Ito T, Moroi Y, Oba J, Nakahara T, Takeuchi S, Uchi H, Takahara M, Masuda T, Furue M
  • Journal Title: Melanoma Res Volume: 22(1) Pages: 38-44
  • Peer Reviewed
• [Journal Article] The prognostic value of a reverse transcriptase-PCR assay of sentinel lymph node biopsy for patients with cutaneous melanoma: a single-center analysis in Japan. (2012)
  • Author(s): Ito T, Moroi Y, Oba J, Nakahara T, Takeuchi S, Uchi H, Takahara M, Masuda T, Furue M.
  • Journal Title: Melanoma Res. Volume: 22 Issue: 1 Pages: 38-44
  • DOI: 10.1097/cmr.0b013e32834dcfdf
  • Peer Reviewed
• [Journal Article] Expression of milk fat globule epidermal growth factor-VIII may be an indicator of poor prognosis in malignant melanoma (2011)
  • Author(s): Oba J, Moroi Y, Nakahara T, Abe T, Hagihara A, Furue M
  • Journal Title: Br J Dermatol Volume: 165(3) Pages: 506-12
  • Peer Reviewed
• [Journal Article] Expression of c-Kit, p-ERK and cyclin D1 in malignant melanoma: animmunohistochemical study and analysis of prognostic value. (2011)
  • Author(s): Oba J, Nakahara T, Abe T, Hagihara A, Moroi Y, Furue M
  • Journal Title: J Dermatol Sci Volume: 62(2) Pages: 116-23
  • Peer Reviewed
• [Journal Article] CD10-bearing fibroblasts may inhibit skin inflammation by down-modulating substance P. (2011)
  • Author(s): Xie L, Takahara M, Nakahara T, Oba J, Uchi H, Takeuchi S, Moroi Y, Furue M
  • Journal Title: Arch Dermatol Res Volume: 303(1) Pages: 49-55
  • Peer Reviewed
• [Journal Article] Expression of CD10 predicts tumor progression and unfavorable prognosis in malignant melanoma. (2011)
  • Author(s): Oba J, Nakahara T, Hayashida S, Kido M, Xie L,Takahara M, Uchi H, Miyazaki S, Abe T, Hagihara A, Moroi Y, Furue M
  • Journal Title: J Am Acad Dermatol. Volume: 65 Pages: 1152-1160
  • Peer Reviewed
• [Presentation] マウス悪性黒色腫浸潤樹状細胞の解析 (2012)
  • Author(s): 中原剛士 師井洋一 古江増隆
  • Organizer: 第22回樹状細胞研究会
  • Place of Presentation: 福島
  • Year and Date: 2012-06-15
• [Presentation] マウス悪性黒色腫浸潤樹状細胞の解析
  • Author(s): 中原剛士
  • Organizer: 第22回樹状細胞研究会
  • Place of Presentation: 福島
• [Presentation] マウス悪性黒色腫浸潤樹状細胞の経時的変化とその意義
  • Author(s): 中原剛士
  • Organizer: 第23回樹状細胞研究会
  • Place of Presentation: 京都
• [Remarks] 九州大学医学部皮膚科学教室
  • URL: http://www.kyudai-derm.org/
• [Remarks] 九州大学医学部皮膚科学教室
  • URL: http://www.kyudai-derm.org/