Research for anti-inflammatory and redox effects of antifungal drug in the skin

• Project/Area Number: 23791277
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kyushu University
• Principal Investigator: TAKAHARA Masakazu 九州大学, 大学病院, 講師 (20398093)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 皮膚炎症 / 再生学 / 活性酸素 / 抗真菌薬 / 芳香族炭化水素受容体 / 表皮細胞 / アゾール / AhR

Research Abstract

We demonstrated that ketoconazole exerted anti-inflammatory effects in addition to an anti-fungal effect in human skin keratinocytes. The effects are dependent on activation of aryl hydrocarbon receptor (AhR), which induced Nrf2 and NQO1 leading to inhibition of ROS, IL-8 production, and DNA damage

Research Products

• [Journal Article] ビデンス・ピローサエキスによるヒト表皮細胞のNrf-2およびNqo1産生亢進 (2013)
  • Author(s): 高原正和、北和代、永江航之介、八谷顕子、日高らん、磯谷智世、土井和子、竹井賢二郎、古江増隆
  • Journal Title: 西日本皮膚科 Volume: 75(3)
  • NAID: 40019744983
  • Peer Reviewed
• [Journal Article] Identification of ketoconazole as an AhR-Nrf2 activator in cultured human keratinocytes: the basis of its anti-inflammatory effect (2012)
  • Author(s): Gaku Tsuji, Masakazu Takahara, Hiroshi Uchi, Tetsuo Matsuda, Takahito Chiba, Satoshi Takeuchi, Fumiko Yasukawa, Yoichi Moroi, Masutaka Furue
  • Journal Title: J Invest Dermatol Volume: 132(1) Pages: 59-68
• [Journal Article] Identification of ketoconazole as an AhR-Nrf2 activator in cultured human keratinocytes: the basis of its anti-inflammatory effect. (2012)
  • Author(s): Gaku Tsuji, Masakazu Takahara, Hiroshi Uchi, Tetsuo Matsuda, Takahito Chiba, Satoshi Takeuchi, Fumiko Yasukawa, Yoichi Moroi, Masutaka Furue.
  • Journal Title: J Invest Dermatol Volume: 132 Pages: 59-68
  • Peer Reviewed
• [Journal Article] ビデンス・ピローサエキスによるヒト表皮細胞のNrf-2およびNqo1産生亢進 西日本皮膚科採用
  • Author(s): 高原正和、北和代、永江航之介、八谷顕子、日高らん、磯谷智世、土井和子、竹井賢二郎、古江増隆
• [Presentation] an extract from artichoke, inhibits UVB-induced oxidative stress in cultured human keratinocytes (2012)
  • Author(s): Tsuji G, Takahara M, Yan X, Hachiya A, Uchi H, Takeuchi S, Yasukawa F, K. Takei K, Moroi Y, Furue M Cynaropicrin
  • Organizer: Society of Investigative Dermatology 72nd Annual meeting
  • Place of Presentation: Raleigh, USA
  • Year and Date: 2012-05-10
• [Presentation] Immunomodulation by tobacco smoking: A possible mechanism exacerbating skin diseases (2012)
  • Author(s): Masakazu Takahara, Gaku Tsuji, Masutaka Furue
  • Organizer: 22nd World Congress of Dermatology
  • Place of Presentation: Seoul, Korea
• [Presentation] Cynaropicrin, an extract from artichoke, inhibits UVB-induced oxidative stress in cultured human keratinocytes. (2012)
  • Author(s): 辻学、高原正和ほか
  • Organizer: Society of Investigative Dermatology 72nd Annual meeting
  • Place of Presentation: Raleigh, USA
• [Presentation] Aryl hydrocarbon receptor modulates keratinocyte differentiation by SIRT1 expression (2011)
  • Author(s): Tsuji G, Takahara M, Uchi H, Takeuchi S, Yasukawa F, Moroi Y, Furue M
  • Organizer: SID 71st Annual Meeting
  • Place of Presentation: Phoenix, USA
• [Presentation] Ketoconazole activates nuclear factor-erythroid 2-related factor-2 (Nrf2) via aryl hydrocarbon receptor signaling pathway: A possible mechanism of anti-inflammatory effect (2011)
  • Author(s): Tsuji G, Takahara M, Matsuda T, Uchi H, Takeuchi S, Mitoma C, Moroi Y, Furue M.
  • Organizer: 22nd World Congress of Dermatology
  • Place of Presentation: Seoul, Korea
• [Presentation] Ketoconazole activates nuclear factor-erythroid 2-related factor-2 (Nrf2) via aryl hydrocarbon receptor signaling pathway: A possible mechanism of anti-inflammatory effect. (2011)
  • Author(s): Tsuji G, Takahara M, Matsuda T, Uchi H, Takeuchi S, Mitoma C, Moroi Y, Furue M.
  • Organizer: 22nd World Congress of Dermatology
  • Place of Presentation: Seoul, Korea
• [Presentation] Immunomodulation by tobacco smoking: A possible mechanism exacerbating skin diseases (2011)
  • Author(s): Takahara M, Tsuji G, Furue M.
  • Organizer: 22nd World Congress of Dermatology(招待講演)
  • Place of Presentation: Seoul, Korea