Does Epiplakin modulate the malignant feature of tumor?

• Project/Area Number: 23791281
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Oita University
• Principal Investigator: ISHIKAWA Kazushi 大分大学, 医学部皮膚科, 助教 (80600452)
• Research Collaborator: FUJIWARA Sakuhei 大分大学, 医学部皮膚科, 教授 (90181411)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: アクチン / エピプラキン / 細胞遊走 / 発現抑制 / HeLa 細胞 / 細胞内シグナル

Research Abstract

To study the function of EPPK, we developed EPPK knock-down (KD) and EPPK-overexpressing HeLa cells and analyzed cellular phenotypes and motility by fluorescence/ differential interference contrast time-lapse microscopy and immunolocalization of actin and vimentin. Cellular motility of EPPK-KD cells was significantly elevated, but that of EPPK-overexpressing cells was obviously depressed. Many spike-like projections were observed on EPPK-KD cells, with fewer such structures on overexpressing cells. By contrast, in EPPK-KD cells, expression of E-cadherin was unchanged but vimentin fibers were thinner and sparser than in controls, and they were more concentrated at the peri -nucleus, as observed in migrating keratinocytes at wound edges in EPPK-/-mice. Our results suggest that EPPK is associated with the machinery for cellular motility and contributes to tissue architecture via the rearrangement of intermediate filaments.

Research Products

• [Journal Article] Epiplakin modifies the motility of the HeLa cells and accumulates at the outer surfaces of 3-D cell clusters (2013)
  • Author(s): Shimada H, Nambu-Niibori A, Wilson-Morifuji M, Mizuguchi S, Araki N, Sumiyoshi H, Sato M, Mezaki Y, Senoo H, Ishikawa K, Hatano Y, Okamoto O, Fujiwara S
  • Journal Title: J Dermatol Volume: 40(4) Pages: 249-58
  • Peer Reviewed
• [Journal Article] Increased fragility, impaired differentiation, and acceleration of migration of corneal epithelium of epiplakin-null mice (2013)
  • Author(s): Kokado M, Okada Y, Goto M, Ishikawa K, Miyamoto T, Yamanaka O, Fujiwara S, Saika S
  • Journal Title: Invest Ophthalmol Vis Sci
  • Peer Reviewed
• [Journal Article] Epiplakin modifies the motility of the HeLa cells and accumulates at the outer surfaces of 3-D cell clusters. (2013)
  • Author(s): Shimada H, Nambu-Niibori A, Wilson-Morifuji M, Mizuguchi S, Araki N, Sumiyoshi H, Sato M, Mezaki Y, Senoo H, Ishikawa K, Hatano Y, Okamoto O, Fujiwara S
  • Journal Title: J Dermatol Volume: 40 Issue: 4 Pages: 249-58
  • DOI: 10.1111/1346-8138.12076
  • Peer Reviewed
• [Presentation] Epiplakin modifies the cellular motility and migration (2011)
  • Author(s): Shimada H, Nambu-Niibori A, Wilson-Morifuji M,Mizoguchi S, Araki N, Sumiyoshi H, Ishikawa K, Fujiwara S
  • Organizer: The 36th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Kyoto
• [Presentation] Epiplakin modifies the cellular motility and migration (2011)
  • Author(s): Hiromitsu Shimada, Akiko Nambu-Niibori, Masayo Wilson-Morifuji, Souhei Mizuguchi , Norie Araki, Hideaki Sumiyoshi, Kazushi Ishikawa, Osamu Okamoto, Sakuhei Fujiwara
  • Organizer: The 36th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 京都