Basic study of autologous transplantation of ex vivo expanding macrophages to secondary lymphedema

• Project/Area Number: 23791286
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: ASAI Jun 京都府立医科大学, 医学研究科, 助教 (50438222)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 皮膚病態学 / マクロファージ / リンパ管 / 血管 / 創傷治癒 / リンパ浮腫

Research Abstract

We tried to establish postoperative lymphedema mouse model but it was failed within the period. The possible reasons are (1)difficulty of visualizing lymphatics by blue dye. (2)edema was not significantly observeded because of quaruped walking. (3)difficulty of the detection of lymph nodes. We next used diabetic wound mo del which is one of the established model of impaired lymphatic function. We focused on statin which can be possible stimulator of macrophages. Statin application significantly improve lymphangiogenesis in diabetic wound model via promoting macrophage func tion.

Research Products

• [Journal Article] Topical simvastatin accelerates wound healing in diabetes by enhancing angiogenesis and lymphangiogenesis (2012)
  • Author(s): Asai J, Takenaka H, Hirakawa S, Sakabe J, Hagura A, Kishimoto S, Maruyama K, Kajiya K, Kinoshita S, Tokura Y , and Katoh N
  • Journal Title: Am J Pathol Volume: 181(6) Pages: 2217-24
  • Peer Reviewed
• [Journal Article] Topical application of ex vivo expanded endothelial progenitor cells promotes vascularisation and wound healing in diabetic mice. (2012)
  • Author(s): Asai J, Takenaka H, Ii M, Asahi M, Kishimoto S, Katoh N, Losordo DW
  • Journal Title: Int Wound J
  • Peer Reviewed
• [Journal Article] Increased plasma LIGHT levels in patients with atopic dermatitis (2012)
  • Author(s): Kotani H, Masuda K, Tamagawa-Mineoka R, Nomiyama T, Soga F, Nin M, Asai J, Kishimoto S, Katoh N
  • Journal Title: Clin Exp Immunol Volume: 168(3) Pages: 318-24
  • Peer Reviewed
• [Journal Article] CXCR4 Antagonist AMD3100 Accelerates Impaired Wound Healing in Diabetic Mice (2012)
  • Author(s): Nishimura Y, Ii M, Qin G, Hamada H, Asai J, Takenaka H, Sekiguchi H, Renault MA, Jujo K, Katoh N, Kishimoto S, Ito A, Kamide C, Kenny J, Millay M, Misener S, Thorne T, Losordo DW
  • Journal Title: J Invest Dermatol.
  • Peer Reviewed
• [Journal Article] Topical simvastatin accelerates wound healing in diabetes by enhancing angiogenesis and lymphangiogenesis (2012)
  • Author(s): Jun Asai
  • Journal Title: The American Journal of Pathology Volume: 181 Issue: 6 Pages: 2217-2224
  • DOI: 10.1016/j.ajpath.2012.08.023
  • Peer Reviewed
• [Journal Article] Topical application of ex vivo expanded endothelial progenitor cells promotes vascularisation and wound healing in diabetic mice. (2012)
  • Author(s): Asai J
  • Journal Title: Int Wound J. Volume: -- Issue: 5 Pages: 527-33
  • DOI: 10.1111/j.1742-481x.2012.01010.x
  • Peer Reviewed
• [Journal Article] CXCR4 antagonist AMD3100 accelerates impaired wound healing in diabetic mice. (2012)
  • Author(s): Nishimura Y, Ii M, Qin G, Hamada H, Asai J, Takenaka H, Sekiguchi H, Renault MA, Jujo K, Katoh N, Kishimoto S, Ito A, Kamide C, Kenny J, Millay M, Misener S, Thorne T, Losordo DW.
  • Journal Title: J Invest Dermatol Volume: 132(3 Pt 1) Pages: 711-720
  • Peer Reviewed
• [Journal Article] Giant cell arteritis with polymyalgia rheumatica associated with influenza vaccination. (2011)
  • Author(s): Wada M, Asai J, Asai A, Nakai D, Kishimoto S, Katoh N
  • Journal Title: Giant cell arteritis with polymyalgia rheumatica associated with influenza vaccination. Volume: Nov;38(11) Pages: 1099-101
  • NAID: 10031147146
  • Peer Reviewed
• [Journal Article] Interstitial granulomatous dermatitis? An unusual presentation in the mucosa and periungual skin (2011)
  • Author(s): Nakamura N, Asai J, Daito J, Takenaka H, Katoh N
  • Journal Title: J Dermatol. Volume: 38(4) Pages: 382-5
  • Peer Reviewed
• [Journal Article] Giant cell arteritis with polymyalgia rheumatica associated with influenza vaccination. (2011)
  • Author(s): Wada M, Asai J, Asai A, Nakai D, Kishimoto S, Katoh N.
  • Journal Title: J Dermatol Volume: 38(11) Pages: 1099-1101
  • NAID: 10031147146
  • Peer Reviewed
• [Journal Article] Interstitial granulomatous dermatitis? An unusual presentation in the mucosa and periungual skin. (2011)
  • Author(s): Nakamura N, Asai J, Daito J, Takenaka H, Katoh N.
  • Journal Title: J Dermatol Volume: 38(4) Pages: 382-385
  • Peer Reviewed
• [Presentation] Regulation of Keratinocyte proliferation by podoplanin during cutaneous wound healing in mice. (2012)
  • Author(s): Asai J, Hirakawa S, Sakabe J, Kishida T,Mazda O, Urano T, Suzuki-Inoue K, Tokura Y, Kotoh N.
  • Organizer: The 37th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Naha, Okinawa, Japan
• [Presentation] Regulation of Keratinocyte migration by podoplanin during cutaneous wound healing in mice (2012)
  • Author(s): Asai J, Hirakawa S, Sakabe J, Kishida T,Mazda O, Urano T, Suzuki-Inoue K, Tokura Y, Kotoh N.
  • Organizer: (ESDR 2012, Venice, Italy) J Invest Dermatol
• [Presentation] Regulation of Keratinocyte proliferation by podoplanin during cutaneous wound healing in mice. (2012)
  • Author(s): Asai J
  • Organizer: The 37th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Okinawa Japan
• [Presentation] Regulation of Keratinocyte migration by podoplanin during cutaneous wound healing in mice. (2012)
  • Author(s): Asai J
  • Organizer: 42nd Annual ESDR meeting
  • Place of Presentation: Venice Italy
• [Remarks] 京都府立医科大学皮膚科学教室ホームページ
  • URL: http://kpum-dermatology.jp/
• [Remarks]
  • URL: http://kpum-dermatology.jp/research/