A study of anti-Dsc autoantibodies for pemphigus herpetiformis and pemphigus vegetans

• Project/Area Number: 23791301
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kurume University
• Principal Investigator: ISHII Norito 久留米大学, 医学部, 講師 (80330827)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 皮膚診断学 / 疱疹状天疱瘡 / 増殖性天疱瘡 / 腫瘍随伴性天疱瘡 / デスモコリン / ELISA法 / 皮膚免疫学 / 自己免疫性水疱症

Research Abstract

No consistent immunoassay for IgG autoantibodies to Dscs has been developed. The aim of this study was to develop reliable assays to detect anti-Dsc autoantibodies. We expressed soluble recombinant proteins of human Dsc1-Dsc3 in mammalian cells and examined sera of various types of pemphigus by novel enzyme-linked immunosorbent assays (ELISAs) using the recombinant proteins. We also performed ELISAs of Dsc baculoproteins, cDNA transfection method, and compared the results with those of mammalian ELISAs.
We have successfully established new sensitive and specific mammalian Dsc IgG enzyme-linked immunosorbent assays (ELISAs). Our data show that IgG anti-Dsc autoantibodies are frequently detected and potentially pathogenic in particular types of non-classical pemphigus.

Research Products

• [Journal Article] IgA pemphigus with paraneoplastic pemphigus-like clinical features showing IgA antibodies to desmoglein 1/3 and desmocollin 3, and IgG and IgA antibodies to the basement membrane zone. (2013)
  • Author(s): Ueda A, Ishii N, Temporin K, Yamazaki R, Murakami F, Fukuda S, Hamada T, Dainichi T, Kyoya M, Saito C, Matsunaga R, Kimura S, Kawakami T, Soma Y, Hashimoto T.
  • Journal Title: Clin Exp Dermatol. Volume: 38(4) Issue: 4 Pages: 370-3
  • DOI: 10.1111/ced.12050
  • Peer Reviewed
• [Journal Article] Unique herpetiform bullous dermatosis with IgG antibodies to desmocollins 1/3 and LAD-1. (2013)
  • Author(s): Ueda A, Ishii N, Teye K, Dainichi T, Ohyama B, Hamada T, Tsuruta D, Kamioka N, Mitsui K, Hosaka H, Soh I, Nakada T, Hashimoto T.
  • Journal Title: Br J Dermatol. Volume: in print Issue: 3 Pages: 719-721
  • DOI: 10.1111/bjd.12398
  • Peer Reviewed
• [Journal Article] Two cases of pemphigus vegetans with IgG anti-desmocollin 3 antibodies. (2013)
  • Author(s): Saruta H, Ishii N, Teye K, Ono F, Ohyama B, Koga H, Ohata C, Furumura M, Tsuruta D, Hashimoto T
  • Journal Title: JAMA Dermatol Volume: 149 Issue: 10 Pages: 1209-1213
  • DOI: 10.1001/jamadermatol.2013.5244
  • Peer Reviewed
• [Journal Article] Coexistence of pemphigus herpetiformis with IgG antibodies to desmocollin 1 and pemphigoid with IgG antibodies to BP180 C-terminal domain and laminin γ2. (2013)
  • Author(s): Ohata C, Higashi Y, Yamagami J, Koga H, Ishii N, Kanekura T, Furumura M, Hashimoto T
  • Journal Title: JAMA Dermatol Volume: 149 Issue: 4 Pages: 502-504
  • DOI: 10.1001/jamadermatol.2013.1916
  • Peer Reviewed
• [Journal Article] IgA pemphigus with non-pustular erythematous lesions and IgA antibodies to desmocollins 1 and 2 (2013)
  • Author(s): Arai R, Okuda H, Tsuruta D, Koga H, Teye K, Ishii N, Ohata C, Hashimoto T
  • Journal Title: Eur J Dermatol Volume: 23 Issue: 3 Pages: 362-365
  • DOI: 10.1684/ejd.2013.2046
  • Peer Reviewed
• [Journal Article] Concurrence of bullous pemphigoid and herpetiform pemphigus with igg antibodies to desmogleins 1/3 and desmocollins 1-3. (2012)
  • Author(s): Ohata C, Koga H, Teye K, Ishii N, Hamada T, Dainichi T, Furumura M, Sato M, Sueki H, Hashimoto T.
  • Journal Title: Br J Dermatol Volume: 168(4) Issue: 4 Pages: 879-81
  • DOI: 10.1111/bjd.12019
  • Peer Reviewed