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Effects of ionizing radiation on the pattern recognition receptors.

Research Project

Project/Area Number 23791383
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Radiation science
Research InstitutionHirosaki University

Principal Investigator

YOSHINO Hironori  弘前大学, 大学院・保健学研究科, 助教 (10583734)

Research Collaborator KASHIWAKURA Ikuo  弘前大学, 大学院・保健学研究科, 教授 (00177370)
MONZEN Satoru  弘前大学, 大学院・保健学研究科, 助教 (20514136)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords放射線 / 自然免疫 / 樹状細胞 / パターン認識受容体 / レドックス制御
Research Abstract

Dendritic cells (DCs) play an essential role in the immune system. This study investigated whether DCs from monocytes exposed to ionizing radiation can adequately respond to pathogen-derived components and proinflammatory cytokines. Human monocytes separated from buffy coats were exposed to x-rays and were then differentiated into DCs. DCs were stimulated by lipopolysaccharide (LPS) or proinflammatory cytokine mixture (MIX). The DCs from nonirradiated and X-irradiated monocytes showed maturation after LPS and MIX stimulation as confirmed by findings of surface antigen expression. Upon LPS stimulation, however, the expression levels of CD80 and CD83 on the DCs from the X-irradiated monocytes were lower than those of the DCs from the nonirradiated monocytes. Such reductions were not observed upon MIX stimulation. Similarly, an impairment of matrix metalloproteinase-9 and cytokine production was observed in the LPS-stimulated DCs from the X-irradiated monocytes, whereas these impairments were not observed upon MIX stimulation. The ability of DCs to stimulate T cells was lower in the irradiated group compared with the nonirradiated group despite the type of maturation stimulus. Thus, the present results suggest that the influence of X-irradiation on the maturation of DCs depends on the type of maturation stimulus used and that X-irradiation especially impairs the response of DCs to LPS.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (20 results)

All 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (11 results) Remarks (2 results)

  • [Journal Article] A liquid crystal-related compound induces cell cycle arrest at the G2/M phase and appotosis in the A549 human non-small cell lung cancer cell line2013

    • Author(s)
      Wakasaya T, Yoshino H, Fukushi Y,Yoshizawa A, Kashiwakura I
    • Journal Title

      International Journal of Oncology

      Volume: 42 Pages: 1205-1211

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Liquid crystal-related compound induces cell cycle arrest at G2/M phase and apoptosis in human non-small cancer cell line A5492013

    • Author(s)
      Takuya Wakasaya
    • Journal Title

      International Journal of Oncology

      Volume: 42 Issue: 4 Pages: 1205-1211

    • DOI

      10.3892/ijo.2013.1804

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Response of the Nrf2 protection system in human monocytic cells after ionising irradiation2012

    • Author(s)
      Yoshino H, Kiminarita T, Matsushita Y,Kashiwakura I
    • Journal Title

      Radiation Protection Dosimetry

      Volume: 152 Pages: 104-108

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impairmentof mature dendritic cells derived from X-irradiated human monocytes depends on the type of maturation stimulus used2012

    • Author(s)
      Yoshino H, Kashiwakura I
    • Journal Title

      Radiation Research

      Volume: 178 Pages: 280-288

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impairment of mature dendritic clls derived from X-irradiated human monocytes depends on the type of maturation stimulus used.2012

    • Author(s)
      Hironori Yoshino, et al.
    • Journal Title

      Radiation Research

      Volume: 178 Issue: 4 Pages: 280-288

    • DOI

      10.1667/rr2997.1

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Response of the Nrf2 protection system in human monocytic cells after ionising iIrradiation.2012

    • Author(s)
      Hironori Yoshino, et al.
    • Journal Title

      Radiation Protection Dosimetry

      Volume: 152 Issue: 1-3 Pages: 104-108

    • DOI

      10.1093/rpd/ncs201

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial superoxide production and redox status in human monocytic cells after ionizing irradiation

    • Author(s)
      Yoshino H, Kiminarita T, Matsushita Y, Kashiwakura I
    • Journal Title

      Radiation Emergency Medicine

      Volume: 印刷中

    • NAID

      40019745067

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 液晶関連化合物の抗腫瘍作用及び放射線増感作用の可能性2012

    • Author(s)
      若佐谷拓也,吉野浩教,吉澤篤,柏倉幾郎
    • Organizer
      第50回日本放射線腫瘍学会生物部会学術大会
    • Place of Presentation
      沖縄県宜野湾市
    • Year and Date
      2012-06-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 病原体認識受容体に及ぼす放射線の影響2012

    • Author(s)
      吉野浩教,柏倉幾郎
    • Organizer
      第50回日本放射線腫瘍学会生物部会学術大会
    • Place of Presentation
      沖縄県宜野湾市
    • Year and Date
      2012-06-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Toll様受容体に及ぼす放射線の影響2012

    • Author(s)
      吉野浩教,柏倉幾郎
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] Response of the Nrf2 protection system in human monocytic cells after ionizing irradiation2012

    • Author(s)
      Yoshino H, Kiminarita T, Matsushita Y,Kashiwakura I
    • Organizer
      International Symposium on the Natural Radiation Exposures and Low Dose Radiation Epidemiological Studies
    • Place of Presentation
      HirosakiJapan.
    • Related Report
      2012 Final Research Report
  • [Presentation] 病原体認識受容体に及ぼす放射線の影響2012

    • Author(s)
      吉野 浩教,他
    • Organizer
      第50回日本放射線腫瘍学会生物部会学術大会
    • Place of Presentation
      沖縄
    • Related Report
      2012 Annual Research Report
  • [Presentation] 液晶関連化合物の抗腫瘍作用及び放射線増感作用の可能性2012

    • Author(s)
      若佐谷 拓也,他
    • Organizer
      第50回日本放射線腫瘍学会生物部会学術大会
    • Place of Presentation
      沖縄
    • Related Report
      2012 Annual Research Report
  • [Presentation] Toll様受容体に及ぼす放射線の影響2012

    • Author(s)
      吉野 浩教,他
    • Organizer
      日本放射線影響学会 第55回大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] 放射線照射細胞におけるNrf2生体防御システムの関与2011

    • Author(s)
      吉野浩教,柏倉幾郎
    • Organizer
      日本放射線影響学会第54回大会
    • Place of Presentation
      神戸市
    • Related Report
      2012 Final Research Report
  • [Presentation] Theeffects of ionizing radiation on pattern recognition receptors2011

    • Author(s)
      Yoshino H, Kashiwakura I
    • Organizer
      14th International Congress of RadiationResearch
    • Place of Presentation
      Warsaw, Poland.
    • Related Report
      2012 Final Research Report
  • [Presentation] The effects of ionizing radiation on pattern recognition receptors2011

    • Author(s)
      吉野浩教、柏倉幾郎
    • Organizer
      14th International Congress of Radiation Research
    • Place of Presentation
      ワルシャワ、ポーランド
    • Related Report
      2011 Research-status Report
  • [Presentation] 放射線照射細胞におけるNrf2生体防御システムの関与2011

    • Author(s)
      吉野浩教、柏倉幾郎
    • Organizer
      日本放射線影響学会第54回大会
    • Place of Presentation
      神戸市商工会議所会館
    • Related Report
      2011 Research-status Report
  • [Remarks] 国立大学法人弘前大学大学院保健学研究科保健学専攻医療生命科学領域放射線生命科学分野 柏倉研究室

    • URL

      http://www.hs.hirosaki-u.ac.jp/~kashiwakura/home.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 弘前大学 研究者詳細 吉野浩教

    • URL

      http://hue2.jm.hirosaki-u.ac.jp/view?l=ja&u=100000291

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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