| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
We prepared NODAGA-EGF as a molecular probe and set up the molecular imaging device in Kumamoto University. EGF, which consists of 53 amino acid residues, was synthesized by Fmoc solid-phase method. After elongation of peptide chain, NODAGA(tBu)3 was introduced at N-terminus. Fully protected peptide resin was treated in TFA solution to cleave from resin and remove protection groups. After HPLC purification, disulfide bonds were formed to give the labeling precursor. Next, to perform molecular imaging study, SPECT/CT was set up. We established a method to form stable complex with Ga-67 via DOTA chelator. SPECT device was checked the accuracy of quantification and image resolution. SPECT imaging using 67Ga-DOTA-octreotate was carried out on the mouse implanted with small cell lung tumor cell (H69). As the result, we could observe the accumulation of the Ga-67 in H69 tumor.
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