| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
In this study, we used 30 tumor cell lines and 1 normal embryonic lung fibroblast, and found that carbon-ion (C-ion) irradiation is effective in suppressing invasiveness of many cell lines. However, C-ion irradiation also enhanced the invasiveness of 2 cell lines, PANC-1 and SF126. Among 285 Anticancer Drugs , we found that nitric oxide synthase (NOS) inhibitors and phosphatidylinositol 3-kinases (PI3K) inhibitors were both effective in suppressing irradiated-PANC-1 invasion. Interestingly, most invading PANC-1 cells were nitric oxide (NO)-producing cells, and C-ion irradiation increased the NO-producing cell population, thereby enhancing invasion. Furthermore, NO2- content was increased in irradiated PANC-1 or SF126, whereas those were decreased in irradiated MIAPaCa-2, whose invasiveness was suppressed by C-ion irradiation, suggested the roles of NO in the regulation of irradiation-altered invasiveness.
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