Approach of radiochemistry about cellular lethality in DNA repair-deficient cell lines after heavy-ion irradiation
| Project/Area Number |
23791472
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
HIRAYAMA Ryoichi 独立行政法人放射線医学総合研究所, 重粒子医科学センター, 研究員 (90435701)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 放射線治療生物学 / 重粒子線 / 生物学的効果比 / 酸素増感比 / 低酸素 / OHラジカル / 陽子線 / DNA二本鎖切断修復 / 間接作用 / RBE / OER / DNA損傷修復欠損細胞 / 重粒子 / LET / DNA損傷修復 / 酸素効果 |
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| Research Abstract |
We estimated the effects of indirect and direct actions on cell killing using dimethylsulfoxide (DMSO). CHO, xrs6 and 51D1 cell lines under oxic and hypoxic conditions were exposed to several intensities of high-LET radiation (13 to 200 keV/micrometer) in the presence or absence of DMSO and cell survival was determined using a colony formation assay. Under both oxic and hypoxic conditions, the contribution of direct action to cell killing increased with increasing LET. In all cell lines, the RBEs determined at a survival level of 10% increased with LET, and the OERs decreased with LET. When the RBE and OER were estimated separately for direct action (RBE-D and OER-D) and indirect action (RBE-I and OER-I); the RBE-D was larger than RBE-I and the OER-D was smaller than OER-I. Thus the direct action of heavy-ions yielded a remarkably larger RBE and smaller OER for cell killing than the indirect action.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Indirect action to cell killing by SOBP carbon-ion beams2014
Author(s)
Ryoichi Hirayama, Yoshitaka Matsumoto, Akiko Uzawa, Yumiko Kaneko, Kana Koda, Masakuni Ozaki, Kei Yamashita, Huizi Li, Miho Noguchi, Toshiyuki Shirai, Yoshiya Furusawa
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Journal Title
Journal of Radiation Research
Volume: 55
Issue: suppl 1
Pages: i133-i134
DOI
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[Presentation] High biological effectiveness depends on the direct action by heavy ions2012
Author(s)
Ryoichi Hirayama, Yoshitaka Matsumoto, Kana Koda, Akiko Uzawa, Miho Noguchi, Yuki Kase, Atsushi Ito, Naruhiro Matsufuji, Ryuichi Okayasu, Yoshiya Furusawa
Organizer
39th Annual Meeting of the European Radiation Research Society
Place of Presentation
Vietri sul Mare, Italy
Year and Date
2012-10-18
Related Report
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[Presentation] Evaluation of cell survival and micronucleus formation in clamped and non-clamped solid tumors exposed to carbon-ion beams in comparison to X-rays2012
Author(s)
Ryoichi Hirayama, Akiko Uzawa, Nobuhiro Takase, Kana Koda, Yoshitaka Matsumoto, Masakuni Ozaki, Miho Noguchi, Yuki Kase, Naruhiro Matsufuji, Sachiko Koike, Shin-ichiro Masunaga, et al.
Organizer
10th International Symposium on Chromosomal Aberrations
Place of Presentation
Amalfi, Italy
Related Report
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[Presentation] Direct action is the key to understand the high biological effects caused by particle beams2011
Author(s)
Ryoichi Hirayama, Yoshitaka Matsumoto, Akiko Uzawa, Nobuhiro Takase, Chizuru Tsuruoka, Mami Wada, Miho Noguchi, Yuki Kase, Naruhiro Matsufuji, Atsushi Ito, Ryuichi Okayasu, Yoshiya Furusawa
Organizer
14th International Congress of Radiation Research
Place of Presentation
Warsaw, Poland
Year and Date
2011-08-30
Related Report
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