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Approach of radiochemistry about cellular lethality in DNA repair-deficient cell lines after heavy-ion irradiation

Research Project

Project/Area Number 23791472
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Radiation science
Research InstitutionNational Institute of Radiological Sciences

Principal Investigator

HIRAYAMA Ryoichi  独立行政法人放射線医学総合研究所, 重粒子医科学センター, 研究員 (90435701)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords放射線治療生物学 / 重粒子線 / 生物学的効果比 / 酸素増感比 / 低酸素 / OHラジカル / 陽子線 / DNA二本鎖切断修復 / 間接作用 / RBE / OER / DNA損傷修復欠損細胞 / 重粒子 / LET / DNA損傷修復 / 酸素効果
Research Abstract

We estimated the effects of indirect and direct actions on cell killing using dimethylsulfoxide (DMSO). CHO, xrs6 and 51D1 cell lines under oxic and hypoxic conditions were exposed to several intensities of high-LET radiation (13 to 200 keV/micrometer) in the presence or absence of DMSO and cell survival was determined using a colony formation assay. Under both oxic and hypoxic conditions, the contribution of direct action to cell killing increased with increasing LET. In all cell lines, the RBEs determined at a survival level of 10% increased with LET, and the OERs decreased with LET. When the RBE and OER were estimated separately for direct action (RBE-D and OER-D) and indirect action (RBE-I and OER-I); the RBE-D was larger than RBE-I and the OER-D was smaller than OER-I. Thus the direct action of heavy-ions yielded a remarkably larger RBE and smaller OER for cell killing than the indirect action.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (29 results)

All 2014 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (26 results) (of which Invited: 3 results)

  • [Journal Article] Indirect action to cell killing by SOBP carbon-ion beams2014

    • Author(s)
      Ryoichi Hirayama, Yoshitaka Matsumoto, Akiko Uzawa, Yumiko Kaneko, Kana Koda, Masakuni Ozaki, Kei Yamashita, Huizi Li, Miho Noguchi, Toshiyuki Shirai, Yoshiya Furusawa
    • Journal Title

      Journal of Radiation Research

      Volume: 55 Issue: suppl 1 Pages: i133-i134

    • DOI

      10.1093/jrr/rrt192

    • Related Report
      2013 Annual Research Report
  • [Journal Article] OH radicals from the indirect actions of X-rays induce cell lethality and mediate the majority of the oxygen enhancement effect2013

    • Author(s)
      Ryoichi Hirayama, Atsushi Ito, Miho Noguchi, Yoshitaka Matsumoto, Akiko Uzawa, Gen Kobashi, Ryuichi Okayasu, Yoshiya Furusawa
    • Journal Title

      Radiation Research

      Volume: 180 Issue: 5 Pages: 514-523

    • DOI

      10.1667/rr13368.1

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of DNA-protein crosslinks (DPCs) by novel direct fluorescence labeling methods: distinct stabilities of aldehyde and radiation-induced DPCs2012

    • Author(s)
      Mahmoud I. Shoulkamy, Toshiaki Nakano, Makiko Ohshima, Ryoichi Hirayama, Akiko Uzawa, Yoshiya Furusawa, Hiroshi Ide
    • Journal Title

      Nucleic Acids Research

      Volume: 40 Issue: 18 Pages: e143-e143

    • DOI

      10.1093/nar/gks601

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 高LET放射線とフリーラジカルの生物影響2013

    • Author(s)
      平山亮一、松本孔貴、鵜澤玲子、金子由美子、平野祥之、尾崎匡邦、山下慶、李惠子、苅谷文子、野口実穂、白井敏之、古澤佳也
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      青森
    • Year and Date
      2013-10-19
    • Related Report
      2013 Final Research Report
  • [Presentation] X線ならびに重粒子線の酸素効果2013

    • Author(s)
      平山亮一、松本孔貴、鵜澤玲子、金子由美子、尾崎匡邦、山下慶、李惠子、苅谷文子、野口実穂、白井敏之、古澤佳也
    • Organizer
      第42回放射線治療による制癌シンポジウム-基礎と臨床の対話-
    • Place of Presentation
      仙台
    • Year and Date
      2013-07-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 重粒子線誘発フリーラジカルの生物影響2013

    • Author(s)
      平山亮一、古澤佳也
    • Organizer
      第6回光・プラズマプロセスのバイオ応用ワークショップ
    • Place of Presentation
      大阪
    • Year and Date
      2013-03-08
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 重粒子線の放射線生物学-3次元スキャニング照射法による生物学的特徴-2013

    • Author(s)
      平山亮一、古澤佳也
    • Organizer
      第11回九州放射線治療システム研究会
    • Place of Presentation
      福岡
    • Year and Date
      2013-01-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 高LET放射線とフリーラジカルの生物影響2013

    • Author(s)
      平山亮一、松本孔貴、鵜澤玲子、金子由美子、平野祥之、尾崎匡邦、山下慶、李惠子、苅谷文子、野口実穂、白井敏之、古澤佳也
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      弘前市、青森
    • Related Report
      2013 Annual Research Report
  • [Presentation] Contribution of indirect action in HSG cells by SOBP carbon-ion beams2013

    • Author(s)
      Ryoichi Hirayama, Yoshitaka Matsumoto, Akiko Uzawa, Yumiko Kaneko, Ozaki Masakuni, Kei Yamashita, Huizi Li, Toshiyuki Shirai, Yoshiya Furusawa
    • Organizer
      40th Annual Meeting of the European Radiation Research Society
    • Place of Presentation
      Dublin, Ireland
    • Related Report
      2013 Annual Research Report
  • [Presentation] X線ならびに重粒子線の酸素効果2013

    • Author(s)
      平山亮一、松本孔貴、鵜澤玲子、金子由美子、尾崎匡邦、山下慶、李惠子、苅谷文子、野口実穂、白井敏之、古澤佳也
    • Organizer
      第42回放射線治療による制癌シンポジウム-基礎と臨床の対話
    • Place of Presentation
      仙台市、宮城
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 炭素線SOBPビームにおける細胞致死と間接作用の寄与2013

    • Author(s)
      平山亮一、松本孔貴、鵜澤玲子、金子由美子、尾崎匡邦、山下慶 、李惠子、白井敏之 、古澤佳也
    • Organizer
      第19回国際癌治療増感研究会
    • Place of Presentation
      文京区、東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Indirect action to cell killing by SOBP carbon-ion beams2013

    • Author(s)
      Ryoichi Hirayama, Yoshitaka Matsumoto, Akiko Uzawa, Yumiko Kaneko, Kana Koda, Ozaki Masakuni, Kei Yamashita, Huizi Li, Toshiyuki Shirai, Yoshiya Furusawa
    • Organizer
      Heavy Ion Therapy and Space Radiation Symposium 2013
    • Place of Presentation
      千葉市、千葉
    • Related Report
      2013 Annual Research Report
  • [Presentation] 重粒子線の放射線生物学-3次元スキャニング照射法による生物学的特徴-2013

    • Author(s)
      平山亮一、古澤佳也
    • Organizer
      第11回九州放射線治療システム研究会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] High biological effectiveness depends on the direct action by heavy ions2012

    • Author(s)
      Ryoichi Hirayama, Yoshitaka Matsumoto, Kana Koda, Akiko Uzawa, Miho Noguchi, Yuki Kase, Atsushi Ito, Naruhiro Matsufuji, Ryuichi Okayasu, Yoshiya Furusawa
    • Organizer
      39th Annual Meeting of the European Radiation Research Society
    • Place of Presentation
      Vietri sul Mare, Italy
    • Year and Date
      2012-10-18
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 酸素効果のメカニズムにおける化学的因子と生物学的因子2012

    • Author(s)
      平山亮一、伊藤敦、安藤興一、古澤佳也
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      仙台
    • Year and Date
      2012-09-06
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 水の放射線分解によって生じたフリーラジカルの生物影響2012

    • Author(s)
      平山亮一
    • Organizer
      平成24年度第1回量子ビーム科学研究施設研究会
    • Place of Presentation
      茨木市
    • Year and Date
      2012-07-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 3次元スキャニング照射法による生物学的利点2012

    • Author(s)
      平山亮一、松本孔貴、鵜澤玲子、幸田華奈、野口実穂、加瀬優紀、松藤成弘、伊藤敦、古澤佳也
    • Organizer
      第50回日本放射線腫瘍学会生物部会学術大会
    • Place of Presentation
      宜野湾市
    • Year and Date
      2012-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Direct action of heavy-ion plays a major role in the high biological effects2012

    • Author(s)
      Ryoichi Hirayama, Yoshitaka Matsumoto, Kana Koda, Akiko Uzawa, Miho Noguchi, Yuki Kase, Naruhiro Matsufuji, Atsushi Ito, Yoshiya Furusawa
    • Organizer
      12th International Workshop on Radiation Damage to DNA
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2012-06-06
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 重粒子線の直接作用がもたらす生物効果2012

    • Author(s)
      平山亮一、松本孔貴、鵜澤玲子、幸田華奈、野口実穂、加瀬優紀、松藤成弘、伊藤敦、古澤佳也
    • Organizer
      第14回癌治療増感研究シンポジウム
    • Place of Presentation
      奈良
    • Year and Date
      2012-02-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Evaluation of cell survival and micronucleus formation in clamped and non-clamped solid tumors exposed to carbon-ion beams in comparison to X-rays2012

    • Author(s)
      Ryoichi Hirayama, Akiko Uzawa, Nobuhiro Takase, Kana Koda, Yoshitaka Matsumoto, Masakuni Ozaki, Miho Noguchi, Yuki Kase, Naruhiro Matsufuji, Sachiko Koike, Shin-ichiro Masunaga, et al.
    • Organizer
      10th International Symposium on Chromosomal Aberrations
    • Place of Presentation
      Amalfi, Italy
    • Related Report
      2012 Research-status Report
  • [Presentation] 最近の低酸素バイオロジー研究について2012

    • Author(s)
      平山亮一
    • Organizer
      平成24年度京都大学原子炉実験所専門研究会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] 水の放射線分解によって生じたフリーラジカルの生物影響2012

    • Author(s)
      平山亮一
    • Organizer
      平成24年度第1回量子ビーム科学研究施設研究会
    • Place of Presentation
      茨木
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 3次元スキャニング照射法による生物学的利点、第50回日本放射線腫瘍学会生物部会学術大会2012

    • Author(s)
      平山亮一、松本孔貴、鵜澤玲子、幸田華奈、野口実穂、加瀬優紀、松藤成弘、伊藤敦、古澤佳也
    • Organizer
      第50回日本放射線腫瘍学会生物部会学術大会
    • Place of Presentation
      宜野湾
    • Related Report
      2012 Research-status Report
  • [Presentation] 重粒子線の直接作用がもたらす生物効果2012

    • Author(s)
      平山亮一
    • Organizer
      第14回癌治療増感研究シンポジウム
    • Place of Presentation
      猿沢荘(奈良県)
    • Related Report
      2011 Research-status Report
  • [Presentation] X線ならびに高LET放射線によって生成されたDNA損傷の低酸素環境下での修復動態2011

    • Author(s)
      平山亮一、松本孔貴、野口実穂、鵜澤玲子、幸田華奈、古澤佳也
    • Organizer
      日本放射線影響学会第54回大会
    • Place of Presentation
      神戸
    • Year and Date
      2011-11-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Direct action is the key to understand the high biological effects caused by particle beams2011

    • Author(s)
      Ryoichi Hirayama, Yoshitaka Matsumoto, Akiko Uzawa, Nobuhiro Takase, Chizuru Tsuruoka, Mami Wada, Miho Noguchi, Yuki Kase, Naruhiro Matsufuji, Atsushi Ito, Ryuichi Okayasu, Yoshiya Furusawa
    • Organizer
      14th International Congress of Radiation Research
    • Place of Presentation
      Warsaw, Poland
    • Year and Date
      2011-08-30
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] X線ならびに高LET放射線によって生成されたDNA損傷の低酸素環境下での修復動態2011

    • Author(s)
      平山亮一
    • Organizer
      日本放射線影響学会第54回大会
    • Place of Presentation
      神戸商工会議所会館(兵庫県)
    • Related Report
      2011 Research-status Report
  • [Presentation] トラック構造と生物効果2011

    • Author(s)
      平山亮一
    • Organizer
      平成23年度京都大学原子炉実験所専門研究会
    • Place of Presentation
      京都大学原子炉実験所(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Oxygen enhancement ratio for different particles2011

    • Author(s)
      Ryoichi Hirayama
    • Organizer
      International Open Laboratory Radiation Quality Research Unit Kick-Off Meeting(招待講演)
    • Place of Presentation
      Darmstadt, Germany
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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