The conquest of anti NeuGc antibody mediated xenogeneic islet rejection through CD47-SIRPα signaling.

• Project/Area Number: 23791484
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Hiroshima University
• Principal Investigator: IDE Kentaro 広島大学, 病院, 助教 (50511565)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 異種移植 / B 細胞 / CD47 / B細胞 / SIRPα / ＳＩＲＰα

Research Abstract

We evaluated the expression of SIRPα on B cell in cytidine monophospho-N-acetylneuraminic acid hydroxylase-deficient mice. There are no SIRPα expressed B cells in the spleen, bone marrow and peripheral blood. However, B cells in the peritoneal cavity only expressed high levels of SIRPα. About 80% of B cells in the peritoneal cavity were B-1 cells. Furthermore, B-1a and B-1b cells expressed high levels of SIRPα. In contrast, B-2 cells did not express the SIRPα.

Research Products

• [Journal Article] Expression of recipient CD47 on rat insulinoma cell xenografts prevents macrophage-mediated rejection through SIRPα inhibitory signaling in mice (2013)
  • Author(s): Yoshifumi Teraoka, Kentaro IdeHiroshi Morimoto , Hiroyuki Tahara , Hideki Ohdan
  • Journal Title: PLoS One Volume: 8(3)
  • Peer Reviewed
• [Journal Article] Expression of recipient CD47 on rat insulinoma cell xenografts prevents macrophage-mediated rejection through SIRPα inhibitory signaling in mice (2013)
  • Author(s): Teraoka Y
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Human CD47 expression permits survival of porcine cells in immunodeficient mice that express SIRPα capable of binding to human CD47. (2012)
  • Author(s): Chunfeng Wang, Hui Wang, Kentaro Ide, Yuantao Wang, Nico Van Rooijen, Hideki Ohdan, Yong-Guang Yang
  • Journal Title: Cell Transplantation Volume: April
  • Peer Reviewed
• [Journal Article] Human CD47 expression permits survival of porcine cells in immunodeficient mice that express SIRPα capable of binding to human CD47 (2011)
  • Author(s): Chunfeng Wang, Hui Wang, Kentaro Ide, Yuantao Wan, Nico Van Rooijen, Hideki Ohdan, Yong-Guang Yang
  • Journal Title: Cell Transplant Volume: 20 Pages: 1915-1920
  • Peer Reviewed
• [Presentation] マクロファージ性拒絶抑制効果に対するレシピエント種 CD47 遺伝子導入の有用性 (2012)
  • Author(s): 寺岡義布史、井手健太郎、森本博司、大段秀樹
  • Organizer: 第15回日本異種移植研究会
  • Place of Presentation: 京都
  • Year and Date: 2012-12-08
• [Presentation] 異種移植におけるマクロファージ性拒絶反応抑制効果に対するレシピエント種 CD47 遺伝子導入の有用性 (2012)
  • Author(s): 寺岡義布史、井手健太郎、森本博司、田中友加、尾上隆司、石山宏平、五十嵐友香、大段秀樹
  • Organizer: 第48回日本移植学会総会
  • Place of Presentation: 名古屋
• [Presentation] Hideki OhdanNecessity for genetic induction of recipient CD47 to prevent macrophage-mediated xenograft rejection through CD47-SIRPα inhibitory signaling (2012)
  • Author(s): Yoshifumi Teraoka, Kentaro Ide, Hiroshi Morimoto
  • Organizer: 2012- XXIV TTS Congress
  • Place of Presentation: Berlin, Germany
• [Presentation] Hideki OhdanGenetic induction of recipient CD47 onxenografts prevents macrophagemediated rejection through CD47-SIRPα inhibitory signaling (2012)
  • Author(s): Yoshifumi Teraoka, Kentaro Ide, Hiroshi Morimoto
  • Organizer: Evidence from an in vivo xenograft mode American Transplant Congress
  • Place of Presentation: Boston, USA
• [Presentation] 異種移植におけるレシピエント種 CD47遺伝子導入によるマクロファージ性拒絶反応抑制効果の in vivo 検証 (2011)
  • Author(s): 寺岡義布史、井手健太郎、森本博司、田中友加、五十嵐友香、安部智之、橋本慎二、平田文宏、堀田龍一、山下正博、田澤宏文、石山宏平、尾上隆司、大段秀樹
  • Organizer: 第14回日本異種移植研究会
  • Place of Presentation: 広島
  • Year and Date: 2011-12-10
• [Presentation] 広島大学における異種移植研究の軌跡と今後の展望 (2011)
  • Author(s): 井手健太郎
  • Organizer: 第14回日本異種移植研究会
  • Place of Presentation: 広島
  • Year and Date: 2011-12-10
• [Presentation] 異種移植におけるレシピエント種 CD47 遺伝子導入によるマクロファージ性拒絶反応抑制効果の in vivo検証 (2011)
  • Author(s): 寺岡義布史、 井手健太郎、 Nabin Basnet、森本博司、田中友加、石山宏平、尾上隆司、五十嵐友香、田澤宏文、堀田龍一、山下正博、橋本慎二、平田文宏、安部智之、大段秀樹
  • Organizer: 第47回日本移植学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-04
• [Presentation] Genetic induction of mouse CD47 on rat insulinoma cells prevents macrophage-mediated xenograftrejection through CD47-SIRPα inhibitory signaling in mice (2011)
  • Author(s): Yoshifumi Teraoka, Kentaro Ide, Hiroyuki Tahara,Nabin Basnet, Hiroshi Morimoto, Hideki Ohdan
  • Organizer: The 11th Joint Annual Congress of the American Society of Transplant Surgeons and The American Society of Transplantation
  • Place of Presentation: Philadelphia, USA
• [Presentation] Genetic induction of mouse CD47 on rat insulinoma cells prevents macrophage-mediated xenograft rejection through CD47-SIRPα inhibitory signaling in mice (2011)
  • Author(s): Yoshifumi Teraoka, Kentaro Ide, Hiroyuki Tahara, Nabin Basnet, Hiroshi Morimoto, Hideki Ohdan
  • Organizer: The 11th Joint Annual Congress of the American Society of Transplant Surgeons and The American Society of Transplantation
  • Place of Presentation: Philadelphia, USA
• [Presentation] 異種移植におけるレシピエント種CD47遺伝子導入によるマクロファージ性拒絶反応抑制効果のin vivo検証 (2011)
  • Author(s): 寺岡義布史、井手健太郎、 Nabin Basnet、森本博司、田中友加、石山宏平、尾上隆司、五十嵐友香、田澤宏文、堀田龍一、山下正博、橋本慎二、平田文宏、安部智之、大段秀樹
  • Organizer: 第47回日本移植学会総会
  • Place of Presentation: 仙台
• [Presentation] 異種移植におけるレシピエント種CD47遺伝子導入によるマクロファージ性拒絶反応抑制効果のin vivo検証 (2011)
  • Author(s): 寺岡義布史、井手健太郎、森本博司、田中友加、五十嵐友香、安部智之、橋本慎二、平田文宏、堀田龍一、山下正博、田澤宏文、石山宏平、尾上隆司、大段秀樹
  • Organizer: 第14回日本異種移植研究会
  • Place of Presentation: 広島