Cancer vaccine therapy using genetically modified induced pluripotent stem cell-derived dendritic cells expressing the TAA gene.
| Project/Area Number |
23791492
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 樹状細胞 / iPS細胞 / 癌ワクチン / アデノウイルスベクター / 腫瘍抗原 / 腫瘍抗原遺伝子 |
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| Research Abstract |
We developed the induced pluripotent stem cell-derived dendritic cells DCs (iPSDCs) from iPS cells and examined the capacity for maturation of iPSDCs compared to that of bone marrow-derived DCs (BMDCs) in addition to the capacity for migration of iPSDCs to lymph nodes. We adenovirally transduced the hgp100 gene, natural tumor antigens, into DCs and immunized mice once with the genetically modified DCs. Our results showed that iPSDCs have an equal capacity to BMDCs in terms of maturation and migration. Furthermore, hgp100-specific CTLs were generated in mice immunized with genetically modified iPSDCs. These CTLs exhibited as high a level of cytotoxicity against B16 cells as BMDCs. Moreover, vaccination with the genetically modified iPSDCs achieved as high a level of therapeutic efficacy as vaccination with BMDCs. Our study clarified that genetically modified iPSDCs have an equal capacity to BMDCs in terms of tumor-associated antigen specific therapeutic antitumor immunity.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Successful treatment of esophageal fistulas with endoscopic injection of alpha-cyanoacrylate monomer.2014
Author(s)
Ojima Toshiyasu, Nakamori Mikihito, Nakamura Masaki, Katsuda Masahiro, Iida Takeshi, Hayata Keiji, Takifuji Katsunari, Iwahashi Makoto, Matsumura Shuichi, Kato Tomoya, Kitadani Junya, Yamaue Hiroki
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Journal Title
Endoscopy
Volume: 46
Issue: S 01
Pages: E62-E63
DOI
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Peer Reviewed / Open Access
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[Journal Article] Inhibition of IL-17A in tumor microenvironment augments cytotoxicity of tumor-infiltrating lymphocytes in tumor-bearing mice2013
Author(s)
Hayata K, Iwahashi M, Ojima T, Katsuda M, Iida T, Nakamori M, Ueda K, Nakamura M, Miyazawa M, Tsuji T,Yamaue H
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Journal Title
PLoS One
Volume: 8(1)
Issue: 1
Pages: e53131-e53131
DOI
Related Report
Peer Reviewed
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[Journal Article] Dendritic cells adenovirally-transduced with full-length mesothelin cDNA elicit mesothelin-specific cytotoxicity against pancreatic cancer cell lines in vitro.2011
Author(s)
Miyazawa M, Iwahashi M, Ojima T, Katsuda M, Nakamura M, Nakamori M, Ueda K, Naka T, Hayata K, Iida T, Yamaue H.
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Journal Title
Cancer Lett
Volume: 305
Pages: 32-9
Related Report
Peer Reviewed
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[Presentation] Clinicopathological characteristics of remnant gastric cancer after a distal gastrectomy2011
Author(s)
Ojima T, Iwahashi M, Nakamori M, Nakamura M, Naka T, Katsuda M, Iida T, Tsuji T, Hayata K, Takifuji K, Yamaue H :
Organizer
The 21st World Congress of the International Association of Surgeons, Gastroenterologists and Oncologists (IASGO),
Place of Presentation
Tokyo
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