Functional analysis of BRCA1/BAP1 complex

• Project/Area Number: 23791504
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: NISHIKAWA Hiroyuki 聖マリアンナ医科大学, 医学(系)研究科(研究院), 研究技術員 (90387077)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 癌抑制遺伝子 / 癌 / 脱ユビキチン化 / ガン抑制遺伝子

Research Abstract

I went to the identification of proteins that bind to the BAP1 in the cell.
It was possible to identify the interaction factor of 51 types. Histone chaperone protein, the chromatin remodeling association was found as multiple interacting factors and BAP1 in this experiment.

Research Products

• [Journal Article] A DNA-Damage Selective Role for BRCA1 E3 Ligase in Claspin Ubiquitylation, CHK1 Activation, and DNA Repair (2012)
  • Author(s): Sato K, Sundaramoorthy E, Rajendra E, Hattori H, Jeyasekharan AD, Ayoub N, Schiess R, Aebersold R, Nishikawa H, Sedukhina AS, Wada H, Ohta T, Venkitaraman AR.
  • Journal Title: Curr Biol Volume: 22 Pages: 1659-66
  • Peer Reviewed
• [Journal Article] DNA相同組換え修復と乳癌 (2012)
  • Author(s): 太田智彦、西川裕之
  • Journal Title: メディカルサイエンスダイジェスト Volume: Vol38(1) Pages: 637-640
  • Peer Reviewed
• [Journal Article] HERC2 Interacts with Claspin and regulates DNA origin firing and replication fork progression (2011)
  • Author(s): Izawa N, Wu W, Sato K, Nishikawa H, Kato A, Boku N, Itoh F, Ohta T.
  • Journal Title: Cancer Res Volume: 71 Pages: 5621-5
  • Peer Reviewed
• [Presentation] Recruitment of phosphorylated NPM1 to sites of DNA damage through RNF8-dependent ubiquitin conjugates (2011)
  • Author(s): Ayaka Koike, Hiroyuki Nishikawa, Wenwen Wu, Yukinori Okada, Ashok R Venkitaraman, Tomohiko Ohta
  • Organizer: EMBO Workshop・The Interface between the Ubiquitin Family and the DNA Damage Response
  • Place of Presentation: Rovinj, Croatia