The elucidation of the novel microRNA-gene pathway regulating the peritoneal dissemination in gastric cancer

• Project/Area Number: 23791550
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Kumamoto University
• Principal Investigator: IWATSUKI Masaaki 熊本大学, 生命科学研究部, 助教 (50452777)
• Research Collaborator: BABA Hideo 熊本大学, 生命科学研究部, 教授 (20240905) ; WATANABE Masayuki 熊本大学, 生命科学研究部, 准教授 (80254639)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: microRNA / 胃癌 / 腹膜播種 / 転移 / 化学療法耐性 / exosome / 消化器癌 / miR-21

Research Abstract

The prognosis of the patients with peritoneal dissemination of gastric cancer still remains dismal. The molecular mechanism of peritoneal dissemination has been not elucidated. microRNA has been reported to be involved in carcinogenesis, progress, metastasis and chemo-resistance. In the current study, we aimed to reveal the novel microRNA- gene pathway in human serum samples regulating the malignant potential of gastrointestinal cancer.

Research Products

• [Journal Article] PIK3CA mutation is associated with a favorable prognosis among patients with curatively resected esophageal squamous cell carcinoma (2013)
  • Author(s): Shigaki H, Baba Y, Watanabe M, Murata A, Ishimoto T, Iwatsuki M, Iwagami S, Nosho K, Baba H.
  • Journal Title: Clin Cancer Res. Volume: 19(9) Issue: 9 Pages: 2451-9
  • DOI: 10.1158/1078-0432.ccr-12-3559
  • Peer Reviewed
• [Journal Article] Plastin3 is a novel marker for circulating tumor cells undergoing the epithelial-mesenchymal transition and is associated with colorectal cancer prognosis (2013)
  • Author(s): Yokobori T, Iinuma H, Shimamura T,Imoto S, Sugimachi K, Ishii H, Iwatsuki M, Ota D, Ohkuma M, Iwaya T,Nishida N, Kogo R, Sudo T, Tanaka F,Shibata K, Toh H, Sato T, Barnard GF,Fukagawa T, Yamamoto S, Nakanishi H, Sasaki S, Miyano S, Watanabe T, Kuwano H, Mimori K, Pantel K, Mori M
  • Journal Title: CancerRes Volume: 73 Pages: 2059-69
  • Peer Reviewed
• [Journal Article] Clinical impact of serum exosomalmicroRNA-21 in human esophagealsquamous cell carcinoma, as a clinicalbiomarker (2013)
  • Author(s): Tanaka Y,Kammohara H,KinoshitaK,Kurashige J,Ishimoto T, Iwatsuki M,Watanabe M, Baba H
  • Journal Title: Cancer Volume: 119 Issue: 6 Pages: 1159-1167
  • DOI: 10.1002/cncr.27895
  • Peer Reviewed
• [Journal Article] Upregulation of ERCC1 and DPD expressions after oxaliplatin-based first-line chemotherapy for metastatic colorectal cancer (2012)
  • Author(s): Baba H, Watanabe M, Okabe H, MiyamotoY, Sakamoto Y, Baba Y, Iwatsuki M,Chikamoto A, Beppu T
  • Journal Title: Br J Cancer Volume: 107 Pages: 1950-5
  • Peer Reviewed
• [Journal Article] PN2 expression predicts response todocetaxel in oesophageal squamouscell carcinoma. (2012)
  • Author(s): Kurashige J, Watanabe M, Iwatsuki M,Kinoshita K, Saito S, Nagai Y,Ishimoto T, Baba Y, Mimori K, Baba H
  • Journal Title: Br J Cancer Volume: 107 Issue: 8 Pages: 1233-8
  • DOI: 10.1038/bjc.2012.396
  • Peer Reviewed
• [Journal Article] Micro RNA- 200b regulates cellproliferation, invasion and migrationby directly targeting ZEB2 in gastriccarcinoma (2012)
  • Author(s): Kurashige J, Kamohara H, Watanabe M,Hiyoshi Y, Iwatsuki M, Tanaka Y,Kinoshita K, Saito S, Baba Y, Baba H
  • Journal Title: Ann Surg Oncol 19 Suppl Volume: 3 Issue: S3 Pages: 656-64
  • DOI: 10.1245/s10434-012-2217-6
  • Peer Reviewed
• [Journal Article] Serum microRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma (2012)
  • Author(s): Kurashige J
  • Journal Title: Journal of Surgical Oncology Volume: (印刷中) Issue: 2 Pages: 188-92
  • DOI: 10.1002/jso.23064
  • Peer Reviewed
• [Presentation] The Identificationof novel microRNAs and Genes in bone marrow regulating gastric cancer metastasis (2012)
  • Author(s): Iwatsuki M, Mimori K, Watanabe M,Fukagawa T, Mori M, Baba H
  • Organizer: 8th International Symposium on Minimal Residual Cancer
  • Place of Presentation: 大阪府 大阪国際会議場
  • Year and Date: 2012-09-23
• [Presentation] トラスツズマブ併用療法により完全寛解が得られた腹膜播種を伴うHER2陽性進行胃癌の 1 例 (2012)
  • Author(s): 糸山明莉、渡邊雅之、岩槻政晃、石本崇胤、岩上志朗、馬場祥史、宮本裕士、馬場秀夫
  • Organizer: 第99回日本消化器病学会九州支部例会
  • Place of Presentation: 佐賀県 ホテルグランデはがくれ
  • Year and Date: 2012-06-29
• [Presentation] Overexpression of microRNA-223 regulates the ubiquitin ligase FBXW7 in esophageal squamous cell carcinoma (2012)
  • Author(s): Kurashige J, Watanabe M, Iwatsuki M,Baba Y, Baba H
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: Chicago McCormick Place West
  • Year and Date: 2012-04-02
• [Presentation] 胃癌における microRNA-200family の発現と臨床病理学的検討 (2012)
  • Author(s): 蔵重淳二、蒲原英伸、渡邊雅之、田中洋平、木下浩一、齋藤誠哉、岩槻政晃、馬場祥史、馬場秀夫
  • Organizer: 第84回日本胃癌学会総会
  • Place of Presentation: 大阪府 大阪国際会議場
  • Year and Date: 2012-02-09
• [Presentation] The Identification of Novel MicroRNAs And Genes In Bone Marrow Regulating Gastric Cancer Metastasis (2012)
  • Author(s): Iwatsuki M, Mimori K, WatanabeM, MoriM, Baba H
  • Organizer: 6th Annual Academic Surgical Congress
  • Place of Presentation: California, USA
  • Year and Date: 2012-02-03
• [Presentation] 食道癌患者と非癌患者の血清中に存在するエキソソームのmicroRNA発現の比較検討 (2012)
  • Author(s): 田中洋平
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 幕張メッセ国際会議場
• [Presentation] The Identificationof novel microRNAs and Genes in bone marrow regulating gastric cancer metastasis (2011)
  • Author(s): Iwatsuki M, Mimori K, Watanabe M,Fukagawa T, Mori M, Baba H
  • Organizer: the 8th International Symposium onMinimal Residual Cancer
  • Place of Presentation: 大阪府 大阪国際会議場
  • Year and Date: 2011-09-22
• [Presentation] MicroRNA-200B regulates epithelial to mesenchymal transition in gastric carcinoma (2011)
  • Author(s): Kurashige J, Watanabe M, Iwatsuki M,Hiyoshi Y, Kinoshita K, Saito S,Kamohara H, Baba Y, Hayshi N, Baba H
  • Organizer: 9th International Gastric CancerCongress
  • Place of Presentation: COEXConvention Center Seoul Korea
  • Year and Date: 2011-04-22
• [Presentation] 胃癌転移における微量癌細胞と宿主側因子の共存の意義 (2011)
  • Author(s): 岩槻政晃、三森功士、渡邊雅之、深川剛生、森 正樹、馬場秀夫
  • Organizer: 第83回日本胃癌学会総会
  • Place of Presentation: 青森県 青森屋
  • Year and Date: 2011-03-05
• [Presentation] 胃癌における microRNA- 200b による癌浸潤転移の制御機構 (2011)
  • Author(s): 藏重淳二、渡邊雅之、木下浩一、齋藤誠哉、佐藤伸隆、辛島龍一、平島浩太郎、日吉幸晴、岩槻政晃、蒲原英伸、林 尚子、馬場秀夫
  • Organizer: 第111回日本外科学会定期学術集会
  • Place of Presentation: 震災の為紙上開催
• [Presentation] 食道癌患者血清から抽出したエキソソーム内の microRNA の発現解析 (2011)
  • Author(s): 田中洋平、渡邊雅之、藏重淳二、木下浩一、齋藤誠也、佐藤伸隆、平島浩太郎、辛島龍一、岡部弘尚、今村 裕、長井洋平、岩槻政晃、林 尚子、馬場秀夫
  • Organizer: 第111回日本外科学会定期学術集会
  • Place of Presentation: 震災のため紙上開催
• [Presentation] The clinical significance of isolated tumor cell and host side cell in bone marrow for gastric cancer metastasis (2011)
  • Author(s): Iwatsuki M
  • Organizer: 9th International Gastric Cancer Congress
  • Place of Presentation: Seoul Korea