The expression and role of Aquaporin in esophageal squamous cell carcinoma
| Project/Area Number |
23791557
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
ISHII Hiromichi 京都府立医科大学, 医学研究科, 助教 (90515206)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 食道癌 / アクアポリン |
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| Research Abstract |
Aquaporins (AQPs) are water channel proteins that facilitate transcellular water movements. Recent studies have shown that AQP5 is expressed in various cancers, and plays a role in tumor progression. However, its expression and role in esophageal squamous cell carcinoma (ESCC) have not been investigated. We examined the pathophysiologic role of AQP5 in cell proliferation and survival, and also investigated its expression and effects on the prognosis of ESCC patients. AQP5 expression was high in TE2 and TE5 cells, human ESCC cell lines. In these cells, the knockdown of AQP5 using siRNA inhibited cell proliferation and G1-S phase progression, and induced apoptosis. A microarray analysis identified 2,132 genes whose expression was altered by AQP5 knockdown in TE5 cells. Of these, 398 genes had cell proliferation-related functions. Pathway analysis showed that cellular growth and proliferation was the top-ranked signal network. Immunohistochemical staining of 68 ESCC patients showed the expression of AQP5 is associated with tumor size, histological type, and tumor recurrence. In conclusion, the expression of AQP5 in ESCC cells may affect cell proliferation and survival, and impact on the prognosis of ESCC patients.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Use of multidetector-row computed tomography to evaluate branch duct type intraductal papillary mucinous neoplasms of the pancreas: influence on surgical decision-making.2012
Author(s)
Ishii H, Taniguchi H, Shiozaki A, Kuriu Y, Ikoma H, Nakanishi M, Ichikawa D, Fujiwara H, Okamoto K, Ochiai T, Kokuba Y, Sakakura C, Sonoyama T, Otsuji E.
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Journal Title
Hepatogastroenterology
Volume: 59
Issue: 115-116
Pages: 884-888
DOI
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Peer Reviewed
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[Journal Article] Total pancreatectomy for pancreatic carcinoma: evaluation of safety and efficacy.2012
Author(s)
Kitagawa M, Ikoma H, Ochiai T, Ishii H, Shiozaki A, Kuriu Y, Nakanishi M, Ichikawa D, Okamoto K, Fujiwara H, Sakakura C, Kokuba Y, Sonoyama T, Otsuji E.
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Journal Title
Hepatogastroenterology
Volume: 59
Pages: 907-910
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Peer Reviewed
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[Journal Article] Blockade of Chloride Ion Transport Enhances the Cytocidal Effect of Hypotonic Solution in Gastric Cancer Cells2011
Author(s)
Iitaka D, Shiozaki A, Ichikawa D, Kosuga T, Komatsu S, Okamoto K, Fujiwara H, Ishii H, Nakahari T, Marunaka Y, Otsuji E
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Journal Title
J Surg Res
Volume: 176
Issue: 2
Pages: 524-34
DOI
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Peer Reviewed
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[Presentation] ESDによる十二指腸穿孔後、後腹膜膿瘍及び縦隔膿瘍を来し、治療に難渋した一例2011
Author(s)
平島相治, 生駒久視, 石井博道, 村山康利, 小松周平, 塩崎敦, 栗生宣明, 中西正芳, 市川大輔, 岡本和真, 藤原斉, 落合登志哉, 國場幸均, 園山輝久, 大辻英吾
Organizer
日本腹部救急医学会
Place of Presentation
福岡
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