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The expression and role of Aquaporin in esophageal squamous cell carcinoma

Research Project

Project/Area Number 23791557
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

ISHII Hiromichi  京都府立医科大学, 医学研究科, 助教 (90515206)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords食道癌 / アクアポリン
Research Abstract

Aquaporins (AQPs) are water channel proteins that facilitate transcellular water movements. Recent studies have shown that AQP5 is expressed in various cancers, and plays a role in tumor progression. However, its expression and role in esophageal squamous cell carcinoma (ESCC) have not been investigated. We examined the pathophysiologic role of AQP5 in cell proliferation and survival, and also investigated its expression and effects on the prognosis of ESCC patients. AQP5 expression was high in TE2 and TE5 cells, human ESCC cell lines. In these cells, the knockdown of AQP5 using siRNA inhibited cell proliferation and G1-S phase progression, and induced apoptosis. A microarray analysis identified 2,132 genes whose expression was altered by AQP5 knockdown in TE5 cells. Of these, 398 genes had cell proliferation-related functions. Pathway analysis showed that cellular growth and proliferation was the top-ranked signal network. Immunohistochemical staining of 68 ESCC patients showed the expression of AQP5 is associated with tumor size, histological type, and tumor recurrence. In conclusion, the expression of AQP5 in ESCC cells may affect cell proliferation and survival, and impact on the prognosis of ESCC patients.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (22 results)

All 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (14 results)

  • [Journal Article] The expression and role of Aquaporin 5 in esophageal squamous cell carcinoma2013

    • Author(s)
      H. Shimizu, A. Shiozaki, E. Otsuji, et al
    • Journal Title

      J Gastroenterol

      Volume: (印刷中) Issue: 4 Pages: 655-666

    • DOI

      10.1007/s00535-013-0827-9

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of the cytocidal effects of hypotonic solution using a chloride channel blocker in pancreatic cancer cells2012

    • Author(s)
      Y. Nako, A. Shiozaki, E. Otsuji, et al
    • Journal Title

      Pancreatology

      Volume: 12(5) Issue: 5 Pages: 440-8

    • DOI

      10.1016/j.pan.2012.08.003

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Use of multidetector-row computed tomography to evaluate branch duct type intraductal papillary mucinous neoplasms of the pancreas: influence on surgical decision-making.2012

    • Author(s)
      Ishii H, Taniguchi H, Shiozaki A, Kuriu Y, Ikoma H, Nakanishi M, Ichikawa D, Fujiwara H, Okamoto K, Ochiai T, Kokuba Y, Sakakura C, Sonoyama T, Otsuji E.
    • Journal Title

      Hepatogastroenterology

      Volume: 59 Issue: 115-116 Pages: 884-888

    • DOI

      10.5754/hge10468

    • Related Report
      2012 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Total pancreatectomy for pancreatic carcinoma: evaluation of safety and efficacy.2012

    • Author(s)
      Kitagawa M, Ikoma H, Ochiai T, Ishii H, Shiozaki A, Kuriu Y, Nakanishi M, Ichikawa D, Okamoto K, Fujiwara H, Sakakura C, Kokuba Y, Sonoyama T, Otsuji E.
    • Journal Title

      Hepatogastroenterology

      Volume: 59 Pages: 907-910

    • Related Report
      2012 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Blockade of Chloride Ion Transport Enhances the Cytocidal Effect of Hypotonic Solution in Gastric Cancer Cells2011

    • Author(s)
      Iitaka D, Shiozaki A, Ichikawa D, Kosuga T, Komatsu S, Okamoto K, Fujiwara H, Ishii H, Nakahari T, Marunaka Y, Otsuji E
    • Journal Title

      J Surg Res

      Volume: 176 Issue: 2 Pages: 524-34

    • DOI

      10.1016/j.jss.2011.10.039

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Risk factors and management of postoperative bile leakage after hepatectomy without bilioenteric anastomosis2011

    • Author(s)
      Ishii H, Sonoyama T, et al
    • Journal Title

      Dig Surg

      Volume: 28 Issue: 3 Pages: 198-204

    • DOI

      10.1159/000324042

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 一時ペーシング後の鼠径部圧迫により下肢急性動脈閉塞症を発症した1例2011

    • Author(s)
      嶋本貴之, 神崎智仁, 工藤道弘, 西村真澄, 石井博道, 當麻敦史, 中村憲司, 伊藤剛, 五十殿弘二, 木村晋三
    • Journal Title

      京都府立与謝の海病院誌

      Volume: 8 Pages: 67-71

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 転落により外傷性横隔膜ヘルニアをきたした1例2011

    • Author(s)
      神崎智仁, 伊藤剛, 中村憲司, 當麻敦史, 石井博道, 西村真澄, 工藤道弘
    • Journal Title

      京都府立与謝の海病院誌

      Volume: 8 Pages: 40-43

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 消化器癌細胞におけるクロライド輸送体制御を利用した低浸透圧細胞破壊効果の増強2013

    • Author(s)
      竹本健一、塩崎敦、石井博道ら
    • Organizer
      日本外科学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] 消化器癌細胞におけるクロライド輸送体制御を利用した低浸透圧細胞破壊効果の増強2013

    • Author(s)
      竹本健一 塩崎敦 市川大輔 小松周平 石井博道 小西博貴 村山康利 栗生宜明 生駒久視 窪田健 中西正芳 岡本和真 藤原斉 落合登志哉 丸中良典 大辻英吾
    • Organizer
      第113回 日本外科学会定期学術集会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Enhancement of cytocidal effect of hypotonic solution using chloride channel blocker in pancreatic cancer cells2012

    • Author(s)
      Nakou Y、Shiozaki A、Ishii H, et al
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      愛知
    • Related Report
      2012 Final Research Report
  • [Presentation] 消化器癌におけるクロライド輸送体制御を利用した蒸留水殺細胞効果の増強2012

    • Author(s)
      名幸義仁 塩崎敦 市川大輔 小松周平 小菅敏幸 飯高大介 石井博道 村山康利 栗生宜明 生駒久視 中西正芳 岡本和真 藤原斉 落合登志哉 國場幸均 丸中良典 大辻英吾
    • Organizer
      第112回 日本外科学会定期学術集会
    • Place of Presentation
      千葉
    • Related Report
      2012 Annual Research Report
  • [Presentation] Expression and prognostic association of XB130/p21 in esophageal squamous cell carcinoma patients2012

    • Author(s)
      Nakou Y、Shiozaki A、Ichikawa D、Komatsu S、Konishi H、Ishii H、Ikoma H、Kubota T、Fujiwara H、Okamoto K、Ochiai T、Marunaka Y、Otsuji E
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] 膵外神経叢郭清 総肝動脈・脾動脈・上腸間膜動脈神経叢郭清2011

    • Author(s)
      石井博道, 小林卓馬, 工藤道弘, 當麻敦史, 中村憲司, 伊藤剛
    • Organizer
      日本臨床外科学会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] 一般病院での腹腔鏡補助下肝切除術の導入 外側区域切除2011

    • Author(s)
      石井博道, 西村真澄, 伊藤剛
    • Organizer
      日本肝胆膵外科学会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] 上腸間膜静脈全周狭窄を伴う膵頭部癌に対する上腸間膜静脈合併膵頭十二指腸切除術2011

    • Author(s)
      石井博道, 工藤道弘, 西村真澄, 當麻敦史, 伊藤剛
    • Organizer
      日本消化器外科学会総会
    • Place of Presentation
      愛知
    • Related Report
      2011 Research-status Report
  • [Presentation] pStage I膵腺扁平上皮癌の1例2011

    • Author(s)
      石井博道, 工藤道弘, 神崎智仁, 西村真澄, 當麻敦史, 中村憲司, 真崎武, 伊藤剛
    • Organizer
      日本膵臓学会
    • Place of Presentation
      青森
    • Related Report
      2011 Research-status Report
  • [Presentation] 当院における大腸印環細胞癌3切除例2011

    • Author(s)
      工藤道弘, 伊藤剛, 中村憲司, 當麻敦史, 石井博道, 小林卓馬
    • Organizer
      日本臨床外科学会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] 術前下部消化管内視鏡検査が有用であった、門脈ガス血症を伴う腸管壊死の例2011

    • Author(s)
      小林卓馬, 伊藤剛, 中村憲司, 當麻敦史, 石井博道, 工藤道弘
    • Organizer
      日本臨床外科学会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] 手術・そこが知りたい!(膵周囲神経叢の郭清) 膵頭部癌に対する膵頭十二指腸切除における2011

    • Author(s)
      西村真澄, 伊藤剛, 石井博道
    • Organizer
      日本肝胆膵外科学会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] 若年者に発症した浸潤性膵管癌の一切除例2011

    • Author(s)
      工藤道弘, 伊藤剛, 中村憲司, 當麻敦史, 石井博道, 西村真澄, 神崎智仁, 真崎武
    • Organizer
      日本膵臓学会
    • Place of Presentation
      青森
    • Related Report
      2011 Research-status Report
  • [Presentation] ESDによる十二指腸穿孔後、後腹膜膿瘍及び縦隔膿瘍を来し、治療に難渋した一例2011

    • Author(s)
      平島相治, 生駒久視, 石井博道, 村山康利, 小松周平, 塩崎敦, 栗生宣明, 中西正芳, 市川大輔, 岡本和真, 藤原斉, 落合登志哉, 國場幸均, 園山輝久, 大辻英吾
    • Organizer
      日本腹部救急医学会
    • Place of Presentation
      福岡
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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