Production of anti-mutated IDH1/2 monoclonal antibodies and its clinical application

• Project/Area Number: 23791584
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: Yamagata University
• Principal Investigator: KATO Yukinari 山形大学, 医学部, 非常勤講師 (00571811)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: モノクローナル抗体 / グリオーマ / IDH1 / IDH2 / 変異型IDH1/2 / R132H / R132S / 免疫組織染色 / IDH1/2

Research Abstract

Isocitrate dehydrogenase IDHmutations occur in low-grade gliomas. However, specific mAbs against mutated IDH1 have not been reported. In this study, we established anti-mutated IDH1-specific mAbs HMab-1 and SMab-1, which are useful in immunohistochemistry of glioma.

Research Products

• [Journal Article] Progressive adult primary glioblastoma in the medulla oblongata with an unmethylated MGMT promoter and without an IDH mutation. (2013)
  • Author(s): Yoshikawa A, Nakada M, Watanabe T, Hayashi Y, Sabit H, Kato Y, Suzuki S, Ooi A, Sato H, Hamada JI.
  • Journal Title: Brain Tumor Pathol. Volume: in press
  • Peer Reviewed
• [Journal Article] imeric anti-podoplanin antibody suppresses tumor metastasis through neutralization and antibody-dependent cellular cytotoxicity (2013)
  • Author(s): Kaneko MK, Kunita A, Abe S, Tsujimoto Y, Fukayama M, Goto K, Sawa Y, Nishioka Y, Kato Y
  • Journal Title: Cancer Sci Volume: 103 Issue: 11 Pages: 1913-1919
  • DOI: 10.1111/j.1349-7006.2012.02385.x
  • Peer Reviewed
• [Journal Article] Progressive adult primary glioblastoma in the medulla oblongata with an unmethylated MGMT promoter and without an IDH mutation (2012)
  • Author(s): Yoshikawa A, Nakada M, Watanabe T, Hayashi Y, Sabit H, Kato Y, Suzuki S, Ooi A, Sato H, Hamada JI
  • Journal Title: Brain Tumor Pathol Volume: (in press) Issue: 3 Pages: 28-28
  • DOI: 10.1007/s10014-012-0118-9
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis-based proteomic subclassification of newly diagnosed glioblastomas with integrated genomic and epigenetic profiles (2012)
  • Author(s): Motomura K, Natsume A, Watanabe R, Ito I, Kato Y, Momota H, Nishikawa R, Mishima K, Nakasu Y, Abe T, Namba H, Nakazato Y, Tashiro H, Takeuchi I, Mori T, Wakabayash T
  • Journal Title: Cancer Sci Volume: 103(10) Pages: 1871-9
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of IDH1 mutation, p53 and internexin as a prognostic factor of glial tumors (2012)
  • Author(s): Takano S, Kato Y, Yamamoto T, Kaneko MK, Ishikawa E, Tsujimoto Y, Matsuda M, Nakai K, Yanagiya R, Morita S, Tsuboi K, Matsumura A
  • Journal Title: J Neurooncol. Volume: 108(3) Pages: 361-73
  • Peer Reviewed
• [Journal Article] IDH1 mutation is a favorable prognostic factor for type 2 gliomatosis cerebri (2012)
  • Author(s): Kwon MJ, Kim ST, Kwon MJ, Kong DS, Lee D, Park S, Kang SY, Song JY, Nam DH, Kato Y, Choi YL, Suh YL
  • Journal Title: Brain Pathol Volume: 22(3) Pages: 307-317
  • Peer Reviewed
• [Journal Article] Mutated IDH1 is a favorable prognostic factor for type 2 gliomatosis cerebri. (2012)
  • Author(s): Kwon MJ, Kim ST, Kwon MJ, Kong DS, Lee D, Park S, Kang SY, Song JY, Nam DH, Kato Y, Choi YL, Suh YL.
  • Journal Title: Brain Pathol. Volume: 22(3) Issue: 3 Pages: 307-317
  • DOI: 10.1111/j.1750-3639.2011.00532.x
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection ofIDH1 mutation, p53, and internexin asprognostic factors of glial tumors (2012)
  • Author(s): Takano S, Kato Y, Yamamoto T, KanekoMK, Ishikawa E, Tsujimoto Y, MatsudaM, Nakai K, Yanagiya R, Morita S,Tsuboi K, Matsumura A.
  • Journal Title: J Neurooncol Volume: 108(3) Issue: 3 Pages: 361-73
  • DOI: 10.1007/s11060-012-0837-0
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis-based proteomic subclassification of newly diagnosed glioblastomas with integrated genomic and epigenetic profiles (2012)
  • Author(s): Motomura K, Natsume A, Watanabe R, Ito I, Kato Y, Momota H, Nishikawa R, Mishima K, Nakasu Y, Abe T, Namba H, Nakazato Y, Tashiro H, Takeuchi I, Mori T, Wakabayash T.
  • Journal Title: Cancer Sci. Volume: 103(10) Issue: 10 Pages: 1871-1879
  • DOI: 10.1111/j.1349-7006.2012.02377.x
  • Peer Reviewed
• [Journal Article] Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin (2012)
  • Author(s): Chiaki Kaj, Yuta Tsujimoto, Mika Kato Kaneko, Yukinari Kato*, and Yoshihiko Sawa*.
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 45 Issue: 4 Pages: 227-237
  • DOI: 10.1267/ahc.12008
  • NAID: 130001854251
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed
• [Journal Article] The expression of podoplanin and classic cadherins in the mouse brain. (2012)
  • Author(s): Chiaki Kaji
  • Journal Title: Journal of Anatomy Volume: 220 Issue: 5 Pages: 435-446
  • DOI: 10.1111/j.1469-7580.2012.01484.x
  • Peer Reviewed
• [Journal Article] Podoplanin expression in advanced atherosclerotic lesions of human aortas (2012)
  • Author(s): Hatakeyama K
  • Journal Title: Thromb Res Volume: 129 Issue: 4 Pages: 70-76
  • DOI: 10.1016/j.thromres.2012.01.003
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of IDH1 mutation, p53 and internexin as a prognostic factor of glial tumors. (2012)
  • Author(s): Takano S, Kato Y, Yamamoto T, Kaneko MK, Ishikawa E, Tsujimoto Y, Matsuda M, Nakai K, Yanagiya R, Morita S, Tsuboi K, Matsumura A.
  • Journal Title: J Neurooncol. Volume: in press
  • Peer Reviewed
• [Journal Article] Podoplanin expression in advanced atherosclerotic lesions of human aortas. (2012)
  • Author(s): Hatakeyama K, Kaneko MK, Kato Y, Ishikawa T, Nishihira K, Tsujimoto Y, Shibata Y, Ozaki Y, Asada Y.
  • Journal Title: Thromb. Res. Volume: 129 Pages: 70-76
  • Peer Reviewed
• [Journal Article] The expression of podoplanin and classical cadherins in the brain. (2012)
  • Author(s): Kaji C, Tomooka M, Kato Y, Kojima H, Sawa Y.
  • Journal Title: J Anat Volume: in press
• [Journal Article] Mutated IDH1 Is a Favorable Prognostic Factor for Type 2 Gliomatosis Cerebri. (2012)
  • Author(s): Kwon MJ, Kim ST, Kwon MJ, Kong DS, Lee D, Park S, Kang SY, Song JY, Nam DH, Kato Y, Choi YL, Suh YL.
  • Journal Title: Brain Pathol. Volume: in press
  • Peer Reviewed
• [Journal Article] Establishment of a novel monoclonal antibody SMab-1 specific for IDH1 R132S mutation (2011)
  • Author(s): Kaneko MK, Tian W, Takano S, Suzuki H, Sawa Y, Hozumi Y, Goto K, Yamazaki K, Kato Y
  • Journal Title: Biochem Biophys Res Commun Volume: 406(4) Pages: 608-613
  • Peer Reviewed
• [Journal Article] Detection of IDH1 mutation in human gliomas: comparison of immunohistochemistry and sequencing (2011)
  • Author(s): Takano S, Tian W, Matsuda M, Yamamoto T, Ishikawa E, Kaneko MK, Yamazaki K, Kato Y, Matsumura A
  • Journal Title: Brain Tumor Pathology Volume: 28(2) Pages: 115-123
  • Peer Reviewed
• [Journal Article] Establishment of a novel monoclonal antibody SMab-1 specific for IDH1 R132S mutation (2011)
  • Author(s): Kaneko MK, Tian W, Takano S, Suzuki H, Sawa Y, Hozumi Y, Goto K, Yamazaki K,Kitanaka C, Kato Y
  • Journal Title: Biochem Biophys Res Commun. Volume: 406 Pages: 608-613
  • Peer Reviewed
• [Journal Article] Detection of IDH1 mutation in human gliomas: comparison of immunohistochemistry and sequencing (2011)
  • Author(s): Takano S, Tian W, Matsuda M, Yamamoto T, Ishikawa E, Kaneko MK, Yamazaki K, Kato Y, Matsumura A.
  • Journal Title: Brain Tumor Pathology Volume: 28 Pages: 115-123
  • Peer Reviewed
• [Presentation] グリオーマの予後診断マーカーとして有用な変異型IDH1/2 に対する特異的抗体の開発 (2012)
  • Author(s): 加藤幸成
  • Organizer: 第32回日本分子腫瘍マーカー研究会
  • Place of Presentation: 札幌、さっぽろ芸文館
  • Year and Date: 2012-09-18
• [Presentation] グリオーマの予後診断マーカーとして有用な変異型IDH1/2に対する特異的抗体の開発
  • Author(s): 加藤幸成
  • Organizer: 第32回日本分子腫瘍マーカー研究会
  • Place of Presentation: さっぽろ芸文館（札幌市）
• [Remarks] 東北大学大学院医学系研究科・加藤研究室
  • URL: http://www.med-tohoku-antibody.com/
• [Remarks] 東北大学大学院医学系研究科・加藤研究室
  • URL: http://www.med-tohoku-antibody.com/