Characterization of USP54 and its splicing variant in glioblastoma cells

• Project/Area Number: 23791591
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: Nagoya University
• Principal Investigator: HASEGAWA Hitoki 名古屋大学, 医学系研究科, 研究員 (10454381)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: グリオーマ / 細胞分裂 / 細胞周期 / 脱ユビキチン化酵素 / USP54 / 細胞質分裂

Research Abstract

We found that USP54 was phosphorylated during mitotic phase. USP54 is localized at cell-cell junction, which is dependent on an association with YWHAE protein. USP54 stabilize ZO-1 protein expression and has important role in cell-cell junction. We identified splicing variant of USP54, whose expression induced aberrant cell morphology and mitosis in glioblastoma cells.

Research Products

• [Journal Article] ALX1 induces snail expression to promote epithelial-to-mesenchymal transition and invasion of ovarian cancer cells. (2013)
  • Author(s): Yuan H, Kajiyama H, Ito S, Yoshikawa N, Hyodo T, Asano E, Hasegawa H, Maeda M, Shibata K, Hamaguchi M, Kikkawa F, Senga T.
  • Journal Title: Cancer Res Volume: 73(5) Pages: 1581-90
  • Peer Reviewed
• [Journal Article] ALX1 induces snail expression to promote epithelial-to-mesenchymal transition and invasion of ovarian cancer cells. (2013)
  • Author(s): Yuan H
  • Journal Title: Cancer Res Volume: 1;73(5) Pages: 1581-90
  • Peer Reviewed
• [Journal Article] Forced expression of miR-143 represses ERK5/c-Myc and p68/p72 signaling in concert with miR-145 in gut tumors of Apc(Min) mice (2012)
  • Author(s): Takaoka Y, Shimizu Y, Hasegawa H, Ouchi Y, Qiao S, Nagahara M, Ichihara M, Lee JD, Adachi K, Hamaguchi M, Iwamoto T
  • Journal Title: PLoS One Volume: 7(8)
  • Peer Reviewed
• [Journal Article] Misshapen-like kinase 1 (MINK1) is a novel component of striatin-interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis. (2012)
  • Author(s): Hyodo T, Ito S, Hasegawa H, Asano E, Maeda M, Urano T, Takahashi M, Hamaguchi M, Senga T.
  • Journal Title: J Biol Chem Volume: 287(30) Pages: 25019-29
  • Peer Reviewed
• [Journal Article] Forced expression of miR-143 represses ERK5/c-Myc and p68/p72 signaling in concert with miR-145 in gut tumors of Apc(Min) mice (2012)
  • Author(s): Takaoka Y
  • Journal Title: PLoS One Volume: 7(8)
  • Peer Reviewed
• [Journal Article] Misshapen-like kinase 1 (MINK1) is a novel component of striatin-interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis. (2012)
  • Author(s): Hyodo T
  • Journal Title: J Biol Chem Volume: 20;287(30) Pages: 25019-29
  • Peer Reviewed
• [Presentation] PLK1 controls cytokinetic furrowing through phosphorylation of an actin/myosin binding protein, supervillin (2012)
  • Author(s): 長谷川仁紀、千賀威
  • Organizer: 分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2012-12-11
• [Presentation] Misshapen-like kinase 1 (MINK1) is a novel component of striatin-interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis (2012)
  • Author(s): 兵頭寿典、長谷川仁紀、千賀威
  • Organizer: 分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2012-12-11
• [Presentation] Misshapen-like kinase 1 (MINK1) is a novel component of striatin-interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis. (2012)
  • Author(s): 兵頭寿典
  • Organizer: 分子生物学会
  • Place of Presentation: 神戸