Comprehensive analysis of glioma LOH regions using next-generation sequencing
| Project/Area Number |
23791608
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 脳腫瘍 / 神経膠腫 / next-generation sequencing / LOH / IDH1 / IDH2 / glioma / glioblastoma / cancer stem cell / micro RNA / genome sequencer / chromosome 10 / 1p/19q LOH |
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| Research Abstract |
Mutation analyses on patient-derived glioma tissues: IDH1/2 mutations and LOH on 1p, 19q, 10, and 17p were analyzed on 162 patient-derived glioma tissues using conventional capillary sequencer. IDH1 mutant primary GBMs showed distinct clinical specificities. By IDH mutation and 1p/19q LOH analyses, grade III oligodendroglial tumors were divided 3 subtypes showing different clinical outcomes. Comprehensive mutation analysis on glioma cancer stem cells using next-generation sequencer: Four patient-derived GBM sphere cultures were analyzed by amplicon PCR using a Truseq Amplicon Cancer Panel, followed by next-generation sequencing using a Miseq instrument (Illumina). Bioinformatics analysis was performed using 'CLC GenomicsWorkbench' software
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Report
(3 results)
Research Products
(16 results)
