Elucidation of the role of the ectopic aquaporin 1 in the onset of hydrocephalus of the kaolin-induced hydrocephalus model in rat

• Project/Area Number: 23791613
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: Nagoya City University
• Principal Investigator: FUJITA Masataka 名古屋市立大学, 大学院・医学研究科, 研究員 (10360637)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 実験脳外科学 / RNA干渉 / アクアポリン / 水頭症 / RNAi / 水チャンネル / 中枢神経 / ラット

Research Abstract

We examined how ectopic AQP1, which increased at the development of hydrocephalus, influenced the hydrocephalus formation using the kaolin-induced hydrocephalus model in rat by RNAi knockdown. As the preparation, production of rabbit anti-AQP1 serum suitable for a Western blot was successful and the arrangement of siRNA suitable for knockdown was found out. However, the suitable method of the injection for in vivo RNAi could not establish within this period, so it remains as a future subject.