Identification of synovial sarcoma initiating cells

• Project/Area Number: 23791621
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Hokkaido University
• Principal Investigator: KIMURA Taichi 北海道大学, 大学院・医学研究科, 助教 (90435959)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 滑膜肉腫 / 腫瘍幹細胞 / 新規治療標的分子

Research Abstract

We demonstrated that the cell surface antigen A is highly expressed in the sphere forming subpopulation of synovial sarcoma and correlate with the expression of stemness genes. Antigen A positive cells possessed high tumorigenic, self-renewing, and re-popularizing activity; therefore we concluded that the Antigen A can enrich synovial sarcoma initiating cells. By immunohistochemical analysis of 42 synovial sarcoma surgical specimens, Antigen A expression was well correlated with poor prognosis. Moreover, the specific inhibitor of antigen A could suppress the proliferation of synovial sarcoma cell-lines in vitro.
In this study, we first detect synovial sarcoma initiating cells and a specific marker, and these findings play a very important role about exploitation the new therapy against synovial sarcoma.

Research Products

• [Journal Article] Inhibition of GSH synthesis potentiates temozolomide-induced bystander effect in glioblastoma (2013)
  • Author(s): Kohsaka, S., Takahashi, K., Tanino, M., Kimura, T., Nishihara, H., Tanaka, S
  • Journal Title: Cancer Letter Volume: 331(1) Pages: 68-75
  • Peer Reviewed
• [Journal Article] Downregulation of miRNA-31 induces taxane resistance in ovarian cancer cells through increase of receptor tyrosinekinase MET (2013)
  • Author(s): Mitamura T., Watari H., Wang L., Kanno H., Hassan MK.,Miyazaki M.,Katoh Y., Kimura T., Tanino M., NishiharaH., Tanaka S., Sakuragi N
  • Journal Title: Oncogenesis Volume: 2
  • Peer Reviewed
• [Journal Article] Expression of CD163 prevents apoptosis through the production of granulocyte colony-stimulating factor in meningioma (2013)
  • Author(s): Kanno H, Nishihara H, Wang L, Yuzawa S, Kobayashi H, Tsuda M, Kimura T, Tanino M, Terasaka S, Tanaka S
  • Journal Title: Neuro Oncol
  • Peer Reviewed
• [Journal Article] Inhibition of GSH synthesis potentiates temozolomide-induced bystander effect in glioblastoma. (2013)
  • Author(s): Kohsaka, S.
  • Journal Title: Cancer Letter Volume: 33 Issue: 1 Pages: 68-75
  • DOI: 10.1016/j.canlet.2012.12.005
  • Peer Reviewed
• [Journal Article] Downregulation of miRNA-31 induces taxane resistance in ovarian cancer cells through increase of receptor tyrosine kinase MET (2013)
  • Author(s): Takashi Mitamura
  • Journal Title: Oncogenesis Volume: 2 Issue: 3 Pages: e40-e40
  • DOI: 10.1038/oncsis.2013.3
  • Peer Reviewed
• [Journal Article] Expression of CD163 prevents apoptosis through the production of granulocyte colony-stimulating factor in meningioma. (2013)
  • Author(s): Kanno H.
  • Journal Title: Neuro Oncol. Volume: Epub ahead of print
  • Peer Reviewed
• [Journal Article] STAT3 inhibition overcomes temozolomide resistance in glioblastoma by downregulating MGMT expression (2012)
  • Author(s): Kohsaka, S., Wang, L., Yachi, K., Mahabir, R., Narita, T., Itoh, T., Tanino, M., Kimura, T., Nishihara H., Tanaka, S
  • Journal Title: Mol Cancer Ther Volume: 11 Pages: 1289-1299
  • NAID: 120005228738
  • Peer Reviewed
• [Journal Article] CD133 negatively regulates tumorigenicity via AKT pathway in synovial sarcoma (2012)
  • Author(s): Kimura, T., Wang, L., Tabu, K., Nishihara, H., Mashita, Y., Kikuchi, N., Tanino, M., Hiraga, H., Tanaka, S
  • Journal Title: Cancerinvest Volume: 30 Pages: 390-397
  • NAID: 120005228733
  • Peer Reviewed
• [Journal Article] Prognostic implication of histological oligodendroglial tumor component: clinicopathological analysis of 111 cases of malignant gliomas (2012)
  • Author(s): Kanno, H., Nishihara, H., Narita, T., Yamaguchi, S., Kobayashi, H., Tanino, M., Kimura, T., Terasaka S., Tanaka, S
  • Journal Title: PLoS One Volume: 7
  • NAID: 120004650825
  • Peer Reviewed
• [Journal Article] CRKL plays a pivotal role intumorigenesis of head and neck squamous cell carcinoma through the regulation of cell adhesion (2012)
  • Author(s): Yanagi, H.,Wang, L.,Nishihara, H., Kimura, T., Tanino, M.,Yanagi, T.,Fukuda, S.,Tanaka, S
  • Journal Title: Biochem Biophys Res Commun Volume: 418 Pages: 104-109
  • Peer Reviewed
• [Journal Article] Successful transplantation of rat hearts subjected to extended cold preservation with a novel preservation solution (2012)
  • Author(s): Wakayama, K.,Fukai, M.,Yamashita, K.,Kimura, T.,Hirokata, G.,Shibasaki, S.,Fukumori, D.,Haga, S. Sugawara, M.,Suzuki, T.,Taniguchi, M.,Shimamura, T.,Furukawa, H.,Ozaki, M.,Kamiyama, T.,Todo, S
  • Journal Title: Transpl Int Volume: 25 Pages: 696-706
  • Peer Reviewed
• [Journal Article] CD133 negatively regulates tumorigenicity via AKT pathway in synovial sarcoma. (2012)
  • Author(s): Kimura, T.
  • Journal Title: Cancer invest Volume: 30 Issue: 5 Pages: 390-397
  • DOI: 10.3109/07357907.2012.672607
  • NAID: 120005228733
  • Peer Reviewed
• [Journal Article] STAT3 inhibition overcomes temozolomide resistance in glioblastoma by downregulating MGMT expression. (2012)
  • Author(s): Kohsaka, S.
  • Journal Title: Mol Cancer Ther Volume: 11 Issue: 6 Pages: 1289-1299
  • DOI: 10.1158/1535-7163.mct-11-0801
  • Peer Reviewed
• [Journal Article] Prognostic implication of histological oligodendroglial tumor component: clinicopathological analysis of 111 cases of malignant gliomas. (2012)
  • Author(s): Kanno, H.,Nishihara, H.,Narita, T.,Yamaguchi, S.,Kobayashi, H.,Tanino, M.,Kimura, T.,Terasaka S.,Tanaka, S.
  • Journal Title: PLoS One. Volume: 7 Issue: 7 Pages: e41669-e41669
  • DOI: 10.1371/journal.pone.0041669
  • NAID: 120004650825
  • Peer Reviewed
• [Journal Article] CRKL plays a pivotal role in tumorigenesis of head and neck squamous cell carcinoma through the regulation of cell adhesion. (2012)
  • Author(s): Yanagi H, Wang L, Nishihara H, Kimura T, Tanino M, Yanagi T, Fukuda S, Tanaka S.
  • Journal Title: Biochem Biophys Res Commun Volume: 418 Issue: 1 Pages: 104-109
  • DOI: 10.1016/j.bbrc.2011.12.142
  • Peer Reviewed
• [Journal Article] Successful transplantation of rat hearts subjected to extended cold preservation with a novel preservation solution (2012)
  • Author(s): Wakayama K
  • Journal Title: Transplant international Volume: 25 Issue: 6 Pages: 696-706
  • DOI: 10.1111/j.1432-2277.2012.01469.x
  • Peer Reviewed
• [Journal Article] A case of clear cell variant of solid-pseudopapillary tumor of the pancreas in an adult male patient. (2012)
  • Author(s): Tanino M, Kohsaka S, Kimura T, Tabu K, Nishihara H, Sawa H, Kawami H, Kamada H, Shimizu M, Tanaka S.
  • Journal Title: Ann Diagn Pathol. Volume: 16 Pages: 134-140
  • Peer Reviewed
• [Journal Article] Expression of O6-methylguanine DNA methyltransferase (MGMT) and immunohistochemical analysis of 12 pineal parenchymal tumors. (2012)
  • Author(s): Kanno, H.,Nishihara, H.,Oikawa, M.,Ozaki, Y.,Murata, J.,Sawamura, Y.,Kato, M.,Kubota, K.,Tanino, M. Kimura, T.,Nagashima, K.,Itoh, T.,Tanaka, S.
  • Journal Title: Neuropathology Volume: 32 Issue: 6 Pages: 647-653
  • DOI: 10.1111/j.1440-1789.2012.01315.x
  • NAID: 120005356854
  • Peer Reviewed
• [Journal Article] CD133 negatively regulates tumorigenicity via AKT pathway in synovial sarcoma (2012)
  • Author(s): Kimura T, Wang L, Tabu K, Nishihara H, Mashita Y, Kikuchi N, Tanino M, Hiraga H, Tanaka S
  • Journal Title: Cancer investigation Volume: 印刷中
  • NAID: 120005228733
  • Peer Reviewed
• [Journal Article] Pancreatic carcinoma with prominent mucin production: A clinicopathologic and immunohistochemical study of 9 cases (2012)
  • Author(s): Saito M, Nishihara H, Tanino M, Kimura T, MatsunoY, Nagashima K, Shimizu M, Tanaka S
  • Journal Title: Open Journal of Pathology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] A population of BJ fibroblasts escaped from Ras-induced senescence susceptible to transformation. (2011)
  • Author(s): Kohsaka S, Sasai K, Takahashi K, Akagi T, Tanino M, Kimura T, Nishihara H, Tanaka S.
  • Journal Title: Biochem Biophys Res Commun. Volume: 410 Issue: 4 Pages: 878-884
  • DOI: 10.1016/j.bbrc.2011.06.082
  • Peer Reviewed
• [Journal Article] A case of atypical teratoid/rhabdoid tumor in an adult, with long survival (2011)
  • Author(s): Takahashi, K., Nishihara, H., Katoh, M., Yoshinaga, T., Mahabir, R., Kanno, H., Kimura, T., Tanino, M., Ikeda, J., Sawamura, Y., Nagashima, K., Tanaka. S
  • Journal Title: Brain Tumor Pathol Volume: 28 Issue: 1 Pages: 71-6
  • DOI: 10.1007/s10014-010-0008-y
  • Peer Reviewed
• [Journal Article] Prenatal diagnosis of short-rib polydactyly syndrome type 3 (Verma-Naumoff type) by three-dimensional helical computed tomography. (2011)
  • Author(s): Yamada T, Nishimura G, Nishida K, Sawai H, Omatsu T, Kimura T, Nishihara H, Shono R, Shimada S, Morikawa M, Mizushima M, Yamada T, Cho K, Tanaka S, Shirato H, Minakami H.
  • Journal Title: J Obstet Gynaecol Res. Volume: 37 Issue: 2 Pages: 151-155
  • DOI: 10.1111/j.1447-0756.2010.01324.x
  • NAID: 120005311807
  • Peer Reviewed
• [Journal Article] A case of lymphoplasmacyte-rich meningioma of the jugular foramen. (2011)
  • Author(s): Kanno H, Nishihara H, Hara K, Ozaki Y, Itoh T, Kimura T, Tanino M, Tanaka S.
  • Journal Title: Brain Tumor Pathol. Volume: 28 Issue: 4 Pages: 341-345
  • DOI: 10.1007/s10014-011-0048-y
  • Peer Reviewed
• [Journal Article] 急激な経過で死亡した新型インフルエンザ肺炎症例の病理学的検討 (2011)
  • Author(s): 瀧山晃広、王磊、谷野美智枝、木村太一、西原広史、田中伸哉
  • Journal Title: 分子呼吸器病 Volume: 51 Pages: 197-202
  • Peer Reviewed
• [Presentation] CD133 は AKT 経路を介して滑膜肉腫の造腫瘍能を負に制御する (2012)
  • Author(s): 木村太一 , 王磊, 平賀博明, 谷野美智枝, 西原広史, 田中伸哉
  • Organizer: 第92回北海道医学大会腫瘍系分科会(第106回北海道癌談話会)
  • Place of Presentation: 北大(札幌)
  • Year and Date: 2012-10-06
• [Presentation] Thedistinct role of CRKI, CRKII and CRKL in tumorigenesis of human head and neck squamous cell carcinoma (2012)
  • Author(s): Hiroshi Nishihara, Hiroko Yanagi, Yusuke Fukushima, Teruki Yanagi, Lei Wang, Taichi Kimura, Mishie Tanino,Masumi Tsuda and Shinya Tanaka
  • Organizer: Mechanisms& Models of Cancer Meeting
  • Place of Presentation: Salk Institute(USA)
• [Presentation] STAT3 inhibition overcomes temozolomide resistance in glioblastoma by downregulation of MGMT expression (2012)
  • Author(s): hinji Kohsaka, Lei Wang, Kazuhiro Yachi, Roshan Mahabir, Mishie Tanino, Taichi Kimura, Hiroshi Nishihara, Shinya Tanaka
  • Organizer: Cold Spring Harbor Laboratory Meeting; Mechanisms and Models of Cancer
  • Place of Presentation: Cold Spring HarborLaboratory (USA)
• [Presentation] Identification and analysis of synovial sarcoma initiating cells. Cold SpringHarbor Laboratory Meeting (2012)
  • Author(s): Taichi Kimura, Lei Wang, Mishie Tanino, Hiroshi Nishihara, Shinya Tanaka
  • Organizer: Mechanisms and Models of Cancer
  • Place of Presentation: Cold Spring Harbor Laboratory (USA)
• [Presentation] from tissues to therapeutic reagents (2012)
  • Author(s): Shinya Tanaka, Shinji Kohsaka, Kouich Tabu, Lei Wang, Masumi Tsuda, Taichi Kimura, Mishie Tanino, Hiroshi Nishihara, Hiromichi Kimura: Molecular and pathological analysis of malignant glioma
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌ほか(札幌)
• [Presentation] The distinct role of CRKI, CRKII and CRKL in tumorigenesis of human head and neck squamous cell carcinoma. (2012)
  • Author(s): Hiroshi Nishihara
  • Organizer: Mechanisms & Models of Cancer Meeting
  • Place of Presentation: Salk Institute（USA）
• [Presentation] STAT3 inhibition overcomes temozolomide resistance in glioblastoma by downregulation of MGMT expression. (2012)
  • Author(s): Shinya Tanaka
  • Organizer: Cold Spring Harbor Laboratory Meeting; Mechanisms and Models of Cancer
  • Place of Presentation: Cold Spring Harbor Laboratory(USA)
• [Presentation] Identification and analysis of synovial sarcoma initiating cells. (2012)
  • Author(s): Taichi Kimura
  • Organizer: Cold Spring Harbor Laboratory Meeting; Mechanisms and Models of Cancer
  • Place of Presentation: Cold Spring Harbor Laboratory(USA)
• [Presentation] Molecular and pathological analysis of malignant glioma: from tissues to therapeutic reagents (2012)
  • Author(s): Shinya Tanaka
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ホテルロイトン札幌 (北海道)
• [Presentation] CD133はAKT経路を介して滑膜肉腫の造腫瘍能を負に制御する (2012)
  • Author(s): 木村太一
  • Organizer: 第92回北海道医学大会腫瘍系分科会
  • Place of Presentation: 北海道大学医学部学友会館フラテ (北海道)
• [Presentation] 癌テーラーメード治療のための統合的個別化病理診断の基盤作 (2011)
  • Author(s): 西原広史、菅野宏美、石川麻倫、湯澤彩夏、大場彩音、木村太一、谷野美智枝、田中伸哉
  • Organizer: 第100回日本病理学会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] IgG4-related sclerosing pachymeningitisの一例 (2011)
  • Author(s): 菅野宏美、谷野美智枝、柳田絵美衣、伊藤智雄、渡邉健太郎、尾崎義丸、伊東民雄、木村太一、西原広史、田中伸哉
  • Organizer: 第100回日本病理学会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] GliomaにおけるMGMT発現調節による抗癌剤耐性解除の可能性の検討 (2011)
  • Author(s): 高阪真路、王磊、谷地一博、成田拓人、木村太一、谷野美智枝、西原広史、田中伸哉
  • Organizer: 第100回日本病理学会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] 肺静脈閉塞症における増殖因子の関与 (2011)
  • Author(s): 谷野美智枝、中村紘子、木村太一、大塚紀幸、深澤雄一郎、西川祐司、池田健、西原広史、田中伸哉
  • Organizer: 第100回日本病理学会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] 急速な経過で死に至ったneuromyelitis opticaの一例 (2011)
  • Author(s): 長井梓、高瀬香奈、菅野宏美、木村太一、竹内朗子、安倍剛典、尾崎義丸、谷野美智枝、西原広史、田中伸哉
  • Organizer: 第100回日本病理学会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] 高齢男性に発生したsmall cell glioblastomaの一例 (2011)
  • Author(s): 高橋健太、菅野宏美、村田純一、木村太一、谷野美智枝、西原広史、長嶋和郎、田中伸哉
  • Organizer: 第100回日本病理学会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] 急激な経過で死に至ったneuromyelitis opticaの一剖検例 (2011)
  • Author(s): 菅野宏美、谷野美智枝、木村太一、西原広史、竹内朗子、阿部剛典、尾崎義丸、田中伸哉
  • Organizer: 第52回日本神経病理学会
  • Place of Presentation: 京都テルサ（京都）
• [Presentation] JCウイルスとメチル化遺伝子結合蛋白MeCP2との相関に関する検討 (2011)
  • Author(s): 高橋健太、王磊、高阪真路、木村太一、白井紗矢、工藤伸一、奴久妻聡一、谷野美智枝、西原広史、澤洋文、長嶋和郎、田中伸哉
  • Organizer: 第52回日本神経病理学会
  • Place of Presentation: 京都テルサ（京都）
• [Presentation] グリオーマにおけるMGMT発現調節による抗癌剤耐性解除の可能性の検討 (2011)
  • Author(s): 高阪真路、王磊、谷地一博、成田拓人、木村太一、谷野美智枝、西原広史、田中伸哉
  • Organizer: 第15回日本がん分子標的治療学会学術集会
  • Place of Presentation: ホテル日航東京（東京）
• [Presentation] 癌化に関与するシグナル伝達アダプター分子Crkの解析 (2011)
  • Author(s): 田中伸哉、王磊、津田真寿美、ロシャン・マハビール、アイマン・ジダン、柳紘子、牧野吉倫、谷野美智枝、木村太一、西原広史
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 名古屋国際会議場（名古屋）
• [Presentation] Involvement Of Adaptor Protein Crk In Malignant Features Of Human Mesothelioma (2011)
  • Author(s): M. Tanino, L. Wang, T. Kimura, H. Nishihara, M. Tsuda, Y. Matsuno, K. Okamoto, S. Yano, S. Tanaka
  • Organizer: ATS(American Thoracic Society)2011
  • Place of Presentation: Colorado convention center （USA）
• [Presentation] Analysis of expression mechanism of MGMT in glioblastoma (2011)
  • Author(s): S Tanaka, S Kohsaka, L Wang, H Kanno, K Takahashi, T Kimura, M Tanino, H Nishihara, K Nagashima
  • Organizer: The 7th Asia Pacific Congress(APIAP 2011)
  • Place of Presentation: Taipei Int'l convention center(Taiwan)
• [Presentation] Analysis of regulatory mechanism of MGMT expression in glioblastoma (2011)
  • Author(s): S Tanaka, SKohsaka, L Wang, H Kanno, K Takahashi, T Kimura, M Tanino, H Nishihara, K Nagashima
  • Organizer: The 2nd Congress of Asian Society of Neuropathology
  • Place of Presentation: 首都医科大学（China）