Functional analysis of Btbd7 in limb formation and chondrogenesis

• Project/Area Number: 23791622
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Hokkaido University
• Principal Investigator: ONODERA Tomohiro 北海道大学, 大学院・医学研究科, 助教 (70547174)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 軟骨再生 / 軟骨修復 / 再生医療 / 軟骨 / 組織修復 / 軟骨細胞

Research Abstract

We hypothesized that Gene expression of Btbd7 has an important role in limb formation and chondrogenesis. Chondrogenic differentiation model of mouse chondrocyte progenitor cell revealed that Btbd7, Snail2, and chemokine SDF-1 showed same expression pattern. These genes were transiently upregulated in chondrogenesis process. Therefore, we have driven forward our study focusing on SDF-1. We revealed that SDF-1 was transiently expressed in healing process of cartilage in vivo. In addition, we clarified that chondrogenesis was promoted by using alginate gel with SDF-1.

Research Products

• [Journal Article] 関節疾患に対する新規注射用治療製剤 (2013)
  • Author(s): 小野寺智洋、岩崎倫政、五十嵐達弥、佃幸憲、祐川敦
  • Journal Title: 化学工業 Volume: 第64巻1月号 Pages: 46-50
• [Journal Article] 関節疾患に対する新規注射用治療製剤 (2013)
  • Author(s): 小野寺 智洋
  • Journal Title: 化学工業 Volume: 64 Pages: 46-50
• [Journal Article] Repair of rabbit osteochondral defects by an acellular technique with an ultrapurified alginate gel containing stromal cell-derived factor-1 (2012)
  • Author(s): Sukegawa A, Iwasaki N, Kasahara Y, Onodera T, Igarashi T, Minami A
  • Journal Title: Tissue Eng Part A Volume: 18(9-10) Pages: 934-45
  • Peer Reviewed
• [Journal Article] Repair of rabbit osteochondral defects by an acellular technique with an ultrapurified alginate gel containing stromal cell-derived factor-1. (2012)
  • Author(s): Sukegawa A, Iwasaki N , Kasahara Y, Onodera T, Igarashi T, Minami A:
  • Journal Title: Tissue Eng Part A. Volume: 18 Issue: 9-10 Pages: 934-945
  • DOI: 10.1089/ten.tea.2011.0380
  • Peer Reviewed
• [Presentation] Repair of Rabbit Osteochondral Defects by an Acellular Technique with an Ultrapurified Alginate Gel Containing Stromal Cell-Derived Factor-1 (2012)
  • Author(s): Sukegawa, N. Iwasaki, Y. Kasahara, T. Onodera, T. Igarashi, A. Minami
  • Organizer: 10th World Congress of the International Cartilage Repair Society
  • Place of Presentation: Fairmont the Queen Elizabeth(Canada)
  • Year and Date: 2012-05-12
• [Presentation] ケモカインSDF-1を用いた無細胞骨軟骨再生医療の可能性 (2012)
  • Author(s): 佑川 敦、岩崎倫政、笠原靖彦、小野寺智洋、五十嵐達弥、三浪明男
  • Organizer: 日本軟骨代謝学会
  • Place of Presentation: 愛知県女性総合センター (愛知県）