• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Tissue regeneration and fluorescent imaging evaluation of adipose-derived stem cells for bone and soft tissue defect after tumor excision

Research Project

Project/Area Number 23791630
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionNagoya City University (2012-2013)
Kanazawa University (2011)

Principal Investigator

HAYASHI Katsuhiro  名古屋市立大学, 医学(系)研究科(研究院), 講師 (80507054)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords骨・軟部腫瘍学 / 脂肪由来幹細胞 / 幹細胞 / 骨再生
Research Abstract

Adipose-derived stem cells (ADSCs) were investigated for regeneration of bone and soft tissue defect after tumor excision. Bone defect model in rat histologically proved better osteogenesis in ADSCs grafted model than in control model. MDM2 was focused for the differences of stem cell differentiation potential. Liposarcoma which has higher amplification of MDM2 showed higher differentiation potential to mesenchymal cells than regular adipose cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report

Research Products

(41 results)

All 2014 2013 2012 2011

All Journal Article (18 results) (of which Peer Reviewed: 15 results,  Open Access: 1 results) Presentation (23 results)

  • [Journal Article] A novel method to apply osteogenic potential of adipose derived stem cells in orthopaedic surgery2014

    • Author(s)
      Fang X, Murakami H, Demura S, Hayashi K, Matsubara H, Kato S, Yoshioka K, Inoue K, Ota T, Shinmura K, Tsuchiya H
    • Journal Title

      PLoS One

      Volume: 9(2)

    • DOI

      10.1371/journal.pone.0088874

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Diagnosis and treatment of low-grade osteosarcoma : experience with nine cases2014

    • Author(s)
      Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Inatani H, Okamoto H, Yamada S, Ikeda H, Sawada-Kitamura S, Nojima T, Ooi A, Otsuka T
    • Journal Title

      Int J Clin Oncol

      Volume: Jul 24 Pages: 731-8

    • DOI

      10.1007/s10147-013-0592-z

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic color-coded fluorescence imaging of the cell-cycle phase, mitosis, and apoptosis demonstrates how caffeine modulates cisplatinum efficacy2013

    • Author(s)
      Miwa S, Yano S, Tome Y, Sugimoto N, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Efimova EV, Fujiwara T, Tsuchiya H, Hoffman RM
    • Journal Title

      J Cell Biochem.

      Volume: 114(11) Pages: 2454-2460

    • DOI

      10.1002/jcb.24593

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] TNF-α and tumor lysate promote the maturation of dendritic cells for immunotherapy for advanced malignant bone and soft tissue tumors2013

    • Author(s)
      Miwa S, Nishida H, Tanzawa Y, Takeuchi A, Yamamoto N, Hayashi K, Kimura H, Tsuchiya H
    • Journal Title

      PLoS One

      Volume: 926

    • DOI

      10.1371/annotation/7c0f14e6-cfba-4f5f-b93d-1a149eaeec96

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Uncultured adipose-derived regenerative cells promote peripheral nerve regeneration2013

    • Author(s)
      Suganuma S, Tada K, Hayashi K, Takeuchi A, Sugimoto N, Ikeda K, Tsuchiya H
    • Journal Title

      J Orthop Sci.

      Volume: 18 Pages: 145-151

    • DOI

      10.1007/s00776-012-0306-9

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Imaging UVC-induced DNA damage response in models of minimal cancer2013

    • Author(s)
      Miwa S, Yano S, Hiroshima Y, Tome Y, Uehara F, Mii S, Efimova EV, Kimura H, Hayashi K, Tsuchiya H, Hoffman RM
    • Journal Title

      J Cell Biochem

      Volume: 114(11) Pages: 2493-9

    • DOI

      10.1002/jcb.24599

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single Cell Time-lapse Imaging of Focus Formation by the DNA Damage-Response Protein 53BP1 after UVC Irradiation of Human Pancreatic Cancer Cells.2013

    • Author(s)
      Miwa S, Tome Y, Yano S, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Tsuchiya H, Bouvet M, Efimova EV, Hoffman RM
    • Journal Title

      Anticancer Res.

      Volume: 33 Pages: 1373-7

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TNF-α and tumor lysate promote the maturation of dendritic cells for immunotherapy for advanced malignant bone and soft tissue tumors2013

    • Author(s)
      Miwa S, Tsuchiya H, et al
    • Journal Title

      PloS One

      Volume: 7

    • DOI

      10.1371/journal.pone.0052926

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 明細胞肉腫の治療成績2012

    • Author(s)
      林克洋,土屋弘行,山本憲男,白井寿治,西田英司,丹澤義一,武内章彦,木村浩明,三輪真嗣,五十嵐健太郎
    • Journal Title

      日整会誌

      Volume: 86 Pages: 675-678

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice2012

    • Author(s)
      Kimura H, Tome Y, Momiyama M, Hayashi K, Tsuchiya H, Bouvet M, Hoffman RM
    • Journal Title

      Int J Cancer

      Volume: 131 Pages: 2027-33

    • DOI

      10.1002/ijc.27475

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Treatment strategies for well-differentiated liposarcomas and therapeutic outcomes2012

    • Author(s)
      Yamamoto N, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Shimozaki S, Kitamura S, Tsuchiya H
    • Journal Title

      Anticancer Res

      Volume: 32 Pages: 1821-5

    • NAID

      120004442385

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Treatment strategies for well-differentiated liposarcomas and therapeutic outcomes.2012

    • Author(s)
      Yamamoto N, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Shimozaki S, Kitamura S, Tsuchiya H.
    • Journal Title

      Anticancer Res.

      Volume: 32 Pages: 1821-1825

    • NAID

      120004442385

    • Related Report
      2012 Research-status Report
  • [Journal Article] Comparison of Cancer-Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-time Imaging in the Live Mouse2011

    • Author(s)
      Hayashi K, Kimura H, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Hasegawa A, Bouvet M, Hoffman RM
    • Journal Title

      Anticancer Res

      Volume: 31 Pages: 3665-72

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Activity of bone morphogenetic protein-7 after treatment at various temperatures : Freezing vs. pasteurization vs. allograft2011

    • Author(s)
      Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y, Kimura H, Miwa S, Takeuchi A, Tsuchiya H
    • Journal Title

      Cryobiology.

      Volume: 63 Pages: 235-239

    • DOI

      10.1016/j.cryobiol.2011.09.001

    • NAID

      120003497601

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional outcomes after total scapulectomy for malignant bone or soft tissue tumors in the shoulder girdle2011

    • Author(s)
      Hayashi K, Karita M, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Tsuchiya H
    • Journal Title

      Int J Clin Oncol

      Volume: 16 Pages: 568-73

    • DOI

      10.1007/s10147-011-0229-z

    • NAID

      10030246962

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 知っておきたい脂肪由来幹細胞移植2011

    • Author(s)
      林克洋,土屋弘行
    • Journal Title

      臨床整形

      Volume: 46 Pages: 864-866

    • Related Report
      2013 Final Research Report
  • [Journal Article] 知っておきたい 脂肪由来幹細胞移植2011

    • Author(s)
      林克洋,土屋弘行
    • Journal Title

      臨床整形

      Volume: 46 Pages: 864-866

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of Cancer-Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-time Imaging in the Live Mouse.2011

    • Author(s)
      Hayashi K
    • Journal Title

      Anticancer Res

      Volume: 31 Pages: 3665-3672

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Long-term histological findings of oncologically sterilized autoclaved bone, irradiated bone and frozen bone2013

    • Author(s)
      Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Tanazawa Y, Inatani H, Okamoto H, Yamada S, Otsuka T
    • Organizer
      低温医学学会総会
    • Place of Presentation
      今池ガスビル(名古屋)
    • Related Report
      2013 Final Research Report
  • [Presentation] Biological reconstruction after excision of bone metastasis using tumor-bearing autografts treated with liquid nitrogen2013

    • Author(s)
      Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Tanazawa Y, Takeuchi A, Inatani H, Otsuka T
    • Organizer
      33^<rd> Hong Kong Orthpedic Association Annual Congress
    • Place of Presentation
      China
    • Related Report
      2013 Final Research Report
  • [Presentation] Long term result of"second-look operation"following unplanned excision of soft tissue malignancy2013

    • Author(s)
      Hayashi K, Yamada S, Inatani H, Okamoto H, Tsuchiya H, Otsuka T
    • Organizer
      International Society of Limb Salvage 17^<th> General Meeting
    • Place of Presentation
      Plazzo Re Enzo (Italy)
    • Related Report
      2013 Final Research Report
  • [Presentation] Leading Technology for in vivo Fluorescent Sarcoma Imaging2013

    • Author(s)
      Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Hoffman RM, Otsuka T
    • Organizer
      The 26th European Musculo Skeletal Oncology Society
    • Place of Presentation
      Swedish Exibition and Congress Center (Sweden)
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨軟部悪性腫瘍に対する直流通電療法の抗腫瘍効果2013

    • Author(s)
      林克洋, 松下廉, 山田聡, 稲谷弘幸, 立松尚衞, 岡本秀貴, 土屋弘行, 大塚隆信
    • Organizer
      第40回日本生体電気・物理刺激研究会
    • Place of Presentation
      ウエスティン都ホテル(京都府)
    • Related Report
      2013 Final Research Report
  • [Presentation] 15年以上経過し筋肉内転移が診断された腎細胞癌の2例2013

    • Author(s)
      林克洋, 村瀬熱紀, 山田聡, 稲谷弘幸, 岡本秀貴, 大塚隆信, 土屋弘行
    • Organizer
      第32回日本画像医学会
    • Place of Presentation
      東京ステーションコンファレンス(東京都)
    • Related Report
      2013 Final Research Report
  • [Presentation] Leading Technology for in vivo Fluorescent Sarcoma Imaging2013

    • Author(s)
      Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Hoffman RM, Otsuka T.
    • Organizer
      European Musculo Skeletal Oncology Society
    • Place of Presentation
      Swedish Exhibition and Congress Center (Gothenburg,Sweden)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Long term result of “second-look operation” following unplanned excision of soft tissue malignancy2013

    • Author(s)
      Hayashi K, Yamada S, Inatani H, Okamoto H, Tsuchiya H, Otsuka T.
    • Organizer
      International Society of Limb Salvage
    • Place of Presentation
      Palazzo Re Enzo (Bologna,Italy)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Biological reconstruction after excision of bone metastasis using tumor-bearing autografts treated with liquid nitrogen2013

    • Author(s)
      Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Tanazawa Y, Takeuchi A, Inatani H, Otsuka T.
    • Organizer
      Hong Kong Orthpedic Association Annual Congress
    • Place of Presentation
      香港会議展覧中心(Hong Kong,中国)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Long-term histological findings of oncologically sterilized autoclaved bone, irradiated bone and frozen bone2013

    • Author(s)
      Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Tanazawa Y,
    • Organizer
      低温医学学会総会
    • Place of Presentation
      今池ガスビル(名古屋)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 骨軟部悪性腫瘍に対する直流通電療法の抗腫瘍効果2013

    • Author(s)
      林克洋 松下廉 山田聡 稲谷弘幸 立松尚衞 岡本秀貴 土屋弘行 大塚隆信
    • Organizer
      第40回日本生体電気・物理刺激研究会
    • Place of Presentation
      ウエスティン都ホテル (京都府)
    • Related Report
      2012 Research-status Report
  • [Presentation] 15年以上経過し筋肉内転移が診断された腎細胞癌の2例2013

    • Author(s)
      林克洋 村瀬熱紀 山田聡 稲谷弘幸 岡本秀貴 大塚隆信 土屋弘行
    • Organizer
      第32回日本画像医学会
    • Place of Presentation
      東京ステーションコンファレンス (東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] Comparison of Sarcoma Cell Seeding in the Lung and Muscle, Visualized by Real-Time Imaging in the Live Mouse2012

    • Author(s)
      Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Hoffman RM
    • Organizer
      AAOS American Academy of Orthopaedic Surgeons
    • Place of Presentation
      Moscone center (USA)
    • Year and Date
      2012-02-09
    • Related Report
      2013 Final Research Report
  • [Presentation] 転移性骨腫瘍の切除後に液体窒素処理した腫瘍骨を用いた生物学的再建2012

    • Author(s)
      林克洋、土屋弘行、山本憲男、白井寿治、西田英司、丹沢義一、武内章彦、木村浩明、三輪真嗣、五十嵐健太郎、稲谷弘幸、下崎真吾、大塚隆信
    • Organizer
      日本低温医学会総会
    • Place of Presentation
      イイノコンファレンスセンター(東京都)
    • Related Report
      2013 Final Research Report
  • [Presentation] 低悪性度の骨肉腫の診断と治療について2012

    • Author(s)
      林克洋、大塚隆信、土屋弘行
    • Organizer
      中部整形外科災害外科学会
    • Place of Presentation
      フェニックスプラザ(福井県)
    • Related Report
      2013 Final Research Report
  • [Presentation] Multicenter Study of Caffeine-potentiated Chemotherapy for Bone and Soft Tissue Sarcoma2012

    • Author(s)
      Hayashi K, Tsuchiya H, Takeuchi A, Otsuka T, Hoshi M, Ieguchi M, Chosa E, Sakamoto T, Tajino T, Yamada H, Yokouchi M, Sakayama K, Kidani T, Isobe K, Maehara H, Tome Y
    • Organizer
      9th Asia Pacific Musculoskeletal Tumor Society Meeting (APMSTS)
    • Place of Presentation
      Hilton Kuala Lumpur (Malaysia)
    • Related Report
      2013 Final Research Report
  • [Presentation] Multicenter Study of Caffeine-potentiated Chemotherapy for Bone and Soft Tissue Sarcoma2012

    • Author(s)
      Hayashi K, Tsuchiya H, Takeuchi A, Otsuka T, Hoshi M, Ieguchi M, Chosa E, Sakamoto T, Tajino T, Yamada H, Yokouchi M, Sakayama K, Kidani T, Isobe K, Maehara H, Tome Y
    • Organizer
      9th Asia Pacific Musculoskeletal Tumor Society Meeting
    • Place of Presentation
      Hilton Kuala Lumpur (Malaysia)
    • Related Report
      2012 Research-status Report
  • [Presentation] 転移性骨腫瘍の切除後に液体窒素処理した腫瘍骨を用いた生物学的再建2012

    • Author(s)
      林克洋、土屋弘行、山本憲男、白井寿治、西田英司、丹沢義一、武内章彦、木村浩明、三輪真嗣、五十嵐健太郎、稲谷弘幸、下崎真吾、大塚隆信
    • Organizer
      日本低温医学会総会
    • Place of Presentation
      イイノコンファレンスセンター(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] 低悪性度の骨肉腫の診断と治療について2012

    • Author(s)
      林克洋、大塚隆信、土屋弘行
    • Organizer
      中部整形外科災害外科学会
    • Place of Presentation
      フェニックスプラザ(福井県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Comparison of Sarcoma Cell Seeding in the Lung and Muscle, Visualized by Real-Time Imaging in the Live Mouse2012

    • Author(s)
      Hayashi K
    • Organizer
      2012 Anual Meeting of The American Academy of Orthopaedic Surgeons
    • Place of Presentation
      Moscone center(USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] シンポジウム「腫瘍の転移」肉腫細胞の可視化モデルを用いた肉腫転移機構の解明2011

    • Author(s)
      林克洋,土屋弘行,山本憲男,白井寿治,西田英司,丹沢義一,武内章彦,木村浩明三輪真嗣,五十嵐健太郎,ロバートMホフマン
    • Organizer
      日本整形外科学会基礎学術集会
    • Place of Presentation
      ベイシア文化ホール(群馬県)
    • Year and Date
      2011-10-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Comparison of Sarcoma Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-Time Imaging in the Live Mouse2011

    • Author(s)
      Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Tanzawa Y, Takeuchi A, Kimura H, Miwa S, Igarashi K, Hoffman RM
    • Organizer
      International Society of Limb Salvage/Musculoskeletal Tumor Society Combined Meeting
    • Place of Presentation
      China National Convention Center (China)
    • Year and Date
      2011-09-17
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 肉腫細胞の可視化モデルを用いた肉腫転移機構の解明2011

    • Author(s)
      林克洋
    • Organizer
      第26回日本整形外科学会基礎学術集会
    • Place of Presentation
      ベイシア文化ホール(群馬県)
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-04   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi