Tissue regeneration and fluorescent imaging evaluation of adipose-derived stem cells for bone and soft tissue defect after tumor excision
Project/Area Number |
23791630
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Orthopaedic surgery
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Research Institution | Nagoya City University (2012-2013) Kanazawa University (2011) |
Principal Investigator |
HAYASHI Katsuhiro 名古屋市立大学, 医学(系)研究科(研究院), 講師 (80507054)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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Keywords | 骨・軟部腫瘍学 / 脂肪由来幹細胞 / 幹細胞 / 骨再生 |
Research Abstract |
Adipose-derived stem cells (ADSCs) were investigated for regeneration of bone and soft tissue defect after tumor excision. Bone defect model in rat histologically proved better osteogenesis in ADSCs grafted model than in control model. MDM2 was focused for the differences of stem cell differentiation potential. Liposarcoma which has higher amplification of MDM2 showed higher differentiation potential to mesenchymal cells than regular adipose cells.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] A novel method to apply osteogenic potential of adipose derived stem cells in orthopaedic surgery2014
Author(s)
Fang X, Murakami H, Demura S, Hayashi K, Matsubara H, Kato S, Yoshioka K, Inoue K, Ota T, Shinmura K, Tsuchiya H
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Diagnosis and treatment of low-grade osteosarcoma : experience with nine cases2014
Author(s)
Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Inatani H, Okamoto H, Yamada S, Ikeda H, Sawada-Kitamura S, Nojima T, Ooi A, Otsuka T
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Journal Title
Int J Clin Oncol
Volume: Jul 24
Pages: 731-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Dynamic color-coded fluorescence imaging of the cell-cycle phase, mitosis, and apoptosis demonstrates how caffeine modulates cisplatinum efficacy2013
Author(s)
Miwa S, Yano S, Tome Y, Sugimoto N, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Efimova EV, Fujiwara T, Tsuchiya H, Hoffman RM
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Journal Title
J Cell Biochem.
Volume: 114(11)
Pages: 2454-2460
DOI
Related Report
Peer Reviewed
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[Journal Article] Imaging UVC-induced DNA damage response in models of minimal cancer2013
Author(s)
Miwa S, Yano S, Hiroshima Y, Tome Y, Uehara F, Mii S, Efimova EV, Kimura H, Hayashi K, Tsuchiya H, Hoffman RM
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Journal Title
J Cell Biochem
Volume: 114(11)
Pages: 2493-9
DOI
Related Report
Peer Reviewed
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[Journal Article] Single Cell Time-lapse Imaging of Focus Formation by the DNA Damage-Response Protein 53BP1 after UVC Irradiation of Human Pancreatic Cancer Cells.2013
Author(s)
Miwa S, Tome Y, Yano S, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Tsuchiya H, Bouvet M, Efimova EV, Hoffman RM
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Journal Title
Anticancer Res.
Volume: 33
Pages: 1373-7
Related Report
Peer Reviewed
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[Journal Article] Comparison of Cancer-Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-time Imaging in the Live Mouse2011
Author(s)
Hayashi K, Kimura H, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Hasegawa A, Bouvet M, Hoffman RM
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Journal Title
Anticancer Res
Volume: 31
Pages: 3665-72
Related Report
Peer Reviewed
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[Journal Article] Activity of bone morphogenetic protein-7 after treatment at various temperatures : Freezing vs. pasteurization vs. allograft2011
Author(s)
Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y, Kimura H, Miwa S, Takeuchi A, Tsuchiya H
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Journal Title
Cryobiology.
Volume: 63
Pages: 235-239
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] Long-term histological findings of oncologically sterilized autoclaved bone, irradiated bone and frozen bone2013
Author(s)
Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Tanazawa Y, Inatani H, Okamoto H, Yamada S, Otsuka T
Organizer
低温医学学会総会
Place of Presentation
今池ガスビル(名古屋)
Related Report
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[Presentation] Leading Technology for in vivo Fluorescent Sarcoma Imaging2013
Author(s)
Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Hoffman RM, Otsuka T.
Organizer
European Musculo Skeletal Oncology Society
Place of Presentation
Swedish Exhibition and Congress Center (Gothenburg,Sweden)
Related Report
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[Presentation] Multicenter Study of Caffeine-potentiated Chemotherapy for Bone and Soft Tissue Sarcoma2012
Author(s)
Hayashi K, Tsuchiya H, Takeuchi A, Otsuka T, Hoshi M, Ieguchi M, Chosa E, Sakamoto T, Tajino T, Yamada H, Yokouchi M, Sakayama K, Kidani T, Isobe K, Maehara H, Tome Y
Organizer
9th Asia Pacific Musculoskeletal Tumor Society Meeting (APMSTS)
Place of Presentation
Hilton Kuala Lumpur (Malaysia)
Related Report
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[Presentation] Multicenter Study of Caffeine-potentiated Chemotherapy for Bone and Soft Tissue Sarcoma2012
Author(s)
Hayashi K, Tsuchiya H, Takeuchi A, Otsuka T, Hoshi M, Ieguchi M, Chosa E, Sakamoto T, Tajino T, Yamada H, Yokouchi M, Sakayama K, Kidani T, Isobe K, Maehara H, Tome Y
Organizer
9th Asia Pacific Musculoskeletal Tumor Society Meeting
Place of Presentation
Hilton Kuala Lumpur (Malaysia)
Related Report
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[Presentation] Comparison of Sarcoma Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-Time Imaging in the Live Mouse2011
Author(s)
Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Tanzawa Y, Takeuchi A, Kimura H, Miwa S, Igarashi K, Hoffman RM
Organizer
International Society of Limb Salvage/Musculoskeletal Tumor Society Combined Meeting
Place of Presentation
China National Convention Center (China)
Year and Date
2011-09-17
Related Report
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