Tissue regeneration and fluorescent imaging evaluation of adipose-derived stem cells for bone and soft tissue defect after tumor excision

• Project/Area Number: 23791630
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Nagoya City University (2012-2013); Kanazawa University (2011)
• Principal Investigator: HAYASHI Katsuhiro 名古屋市立大学, 医学(系)研究科(研究院), 講師 (80507054)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 骨・軟部腫瘍学 / 脂肪由来幹細胞 / 幹細胞 / 骨再生

Research Abstract

Adipose-derived stem cells (ADSCs) were investigated for regeneration of bone and soft tissue defect after tumor excision. Bone defect model in rat histologically proved better osteogenesis in ADSCs grafted model than in control model. MDM2 was focused for the differences of stem cell differentiation potential. Liposarcoma which has higher amplification of MDM2 showed higher differentiation potential to mesenchymal cells than regular adipose cells.

Research Products

• [Journal Article] A novel method to apply osteogenic potential of adipose derived stem cells in orthopaedic surgery (2014)
  • Author(s): Fang X, Murakami H, Demura S, Hayashi K, Matsubara H, Kato S, Yoshioka K, Inoue K, Ota T, Shinmura K, Tsuchiya H
  • Journal Title: PLoS One Volume: 9(2)
  • DOI: 10.1371/journal.pone.0088874
  • Peer Reviewed / Open Access
• [Journal Article] Diagnosis and treatment of low-grade osteosarcoma : experience with nine cases (2014)
  • Author(s): Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Inatani H, Okamoto H, Yamada S, Ikeda H, Sawada-Kitamura S, Nojima T, Ooi A, Otsuka T
  • Journal Title: Int J Clin Oncol Volume: Jul 24 Pages: 731-8
  • DOI: 10.1007/s10147-013-0592-z
  • Peer Reviewed
• [Journal Article] Dynamic color-coded fluorescence imaging of the cell-cycle phase, mitosis, and apoptosis demonstrates how caffeine modulates cisplatinum efficacy (2013)
  • Author(s): Miwa S, Yano S, Tome Y, Sugimoto N, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Efimova EV, Fujiwara T, Tsuchiya H, Hoffman RM
  • Journal Title: J Cell Biochem. Volume: 114(11) Pages: 2454-2460
  • DOI: 10.1002/jcb.24593
  • Peer Reviewed
• [Journal Article] TNF-α and tumor lysate promote the maturation of dendritic cells for immunotherapy for advanced malignant bone and soft tissue tumors (2013)
  • Author(s): Miwa S, Nishida H, Tanzawa Y, Takeuchi A, Yamamoto N, Hayashi K, Kimura H, Tsuchiya H
  • Journal Title: PLoS One Volume: 926
  • DOI: 10.1371/annotation/7c0f14e6-cfba-4f5f-b93d-1a149eaeec96
  • Peer Reviewed
• [Journal Article] Uncultured adipose-derived regenerative cells promote peripheral nerve regeneration (2013)
  • Author(s): Suganuma S, Tada K, Hayashi K, Takeuchi A, Sugimoto N, Ikeda K, Tsuchiya H
  • Journal Title: J Orthop Sci. Volume: 18 Pages: 145-151
  • DOI: 10.1007/s00776-012-0306-9
  • Peer Reviewed
• [Journal Article] Imaging UVC-induced DNA damage response in models of minimal cancer (2013)
  • Author(s): Miwa S, Yano S, Hiroshima Y, Tome Y, Uehara F, Mii S, Efimova EV, Kimura H, Hayashi K, Tsuchiya H, Hoffman RM
  • Journal Title: J Cell Biochem Volume: 114(11) Pages: 2493-9
  • DOI: 10.1002/jcb.24599
  • Peer Reviewed
• [Journal Article] Single Cell Time-lapse Imaging of Focus Formation by the DNA Damage-Response Protein 53BP1 after UVC Irradiation of Human Pancreatic Cancer Cells. (2013)
  • Author(s): Miwa S, Tome Y, Yano S, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Tsuchiya H, Bouvet M, Efimova EV, Hoffman RM
  • Journal Title: Anticancer Res. Volume: 33 Pages: 1373-7
  • Peer Reviewed
• [Journal Article] TNF-α and tumor lysate promote the maturation of dendritic cells for immunotherapy for advanced malignant bone and soft tissue tumors (2013)
  • Author(s): Miwa S, Tsuchiya H, et al
  • Journal Title: PloS One Volume: 7
  • DOI: 10.1371/journal.pone.0052926
  • Peer Reviewed
• [Journal Article] 明細胞肉腫の治療成績 (2012)
  • Author(s): 林克洋,土屋弘行,山本憲男,白井寿治,西田英司,丹澤義一,武内章彦,木村浩明,三輪真嗣,五十嵐健太郎
  • Journal Title: 日整会誌 Volume: 86 Pages: 675-678
• [Journal Article] Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice (2012)
  • Author(s): Kimura H, Tome Y, Momiyama M, Hayashi K, Tsuchiya H, Bouvet M, Hoffman RM
  • Journal Title: Int J Cancer Volume: 131 Pages: 2027-33
  • DOI: 10.1002/ijc.27475
  • Peer Reviewed
• [Journal Article] Treatment strategies for well-differentiated liposarcomas and therapeutic outcomes (2012)
  • Author(s): Yamamoto N, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Shimozaki S, Kitamura S, Tsuchiya H
  • Journal Title: Anticancer Res Volume: 32 Pages: 1821-5
  • NAID: 120004442385
  • Peer Reviewed
• [Journal Article] Treatment strategies for well-differentiated liposarcomas and therapeutic outcomes. (2012)
  • Author(s): Yamamoto N, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Shimozaki S, Kitamura S, Tsuchiya H.
  • Journal Title: Anticancer Res. Volume: 32 Pages: 1821-1825
  • NAID: 120004442385
• [Journal Article] Comparison of Cancer-Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-time Imaging in the Live Mouse (2011)
  • Author(s): Hayashi K, Kimura H, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Hasegawa A, Bouvet M, Hoffman RM
  • Journal Title: Anticancer Res Volume: 31 Pages: 3665-72
  • Peer Reviewed
• [Journal Article] Activity of bone morphogenetic protein-7 after treatment at various temperatures : Freezing vs. pasteurization vs. allograft (2011)
  • Author(s): Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y, Kimura H, Miwa S, Takeuchi A, Tsuchiya H
  • Journal Title: Cryobiology. Volume: 63 Pages: 235-239
  • DOI: 10.1016/j.cryobiol.2011.09.001
  • NAID: 120003497601
  • Peer Reviewed
• [Journal Article] Functional outcomes after total scapulectomy for malignant bone or soft tissue tumors in the shoulder girdle (2011)
  • Author(s): Hayashi K, Karita M, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Tsuchiya H
  • Journal Title: Int J Clin Oncol Volume: 16 Pages: 568-73
  • DOI: 10.1007/s10147-011-0229-z
  • NAID: 10030246962
  • Peer Reviewed
• [Journal Article] 知っておきたい脂肪由来幹細胞移植 (2011)
  • Author(s): 林克洋,土屋弘行
  • Journal Title: 臨床整形 Volume: 46 Pages: 864-866
• [Journal Article] 知っておきたい 脂肪由来幹細胞移植 (2011)
  • Author(s): 林克洋，土屋弘行
  • Journal Title: 臨床整形 Volume: 46 Pages: 864-866
  • Peer Reviewed
• [Journal Article] Comparison of Cancer-Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-time Imaging in the Live Mouse. (2011)
  • Author(s): Hayashi K
  • Journal Title: Anticancer Res Volume: 31 Pages: 3665-3672
  • Peer Reviewed
• [Presentation] Long-term histological findings of oncologically sterilized autoclaved bone, irradiated bone and frozen bone (2013)
  • Author(s): Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Tanazawa Y, Inatani H, Okamoto H, Yamada S, Otsuka T
  • Organizer: 低温医学学会総会
  • Place of Presentation: 今池ガスビル(名古屋)
• [Presentation] Biological reconstruction after excision of bone metastasis using tumor-bearing autografts treated with liquid nitrogen (2013)
  • Author(s): Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Tanazawa Y, Takeuchi A, Inatani H, Otsuka T
  • Organizer: 33^<rd> Hong Kong Orthpedic Association Annual Congress
  • Place of Presentation: China
• [Presentation] Long term result of"second-look operation"following unplanned excision of soft tissue malignancy (2013)
  • Author(s): Hayashi K, Yamada S, Inatani H, Okamoto H, Tsuchiya H, Otsuka T
  • Organizer: International Society of Limb Salvage 17^<th> General Meeting
  • Place of Presentation: Plazzo Re Enzo (Italy)
• [Presentation] Leading Technology for in vivo Fluorescent Sarcoma Imaging (2013)
  • Author(s): Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Hoffman RM, Otsuka T
  • Organizer: The 26th European Musculo Skeletal Oncology Society
  • Place of Presentation: Swedish Exibition and Congress Center (Sweden)
• [Presentation] 骨軟部悪性腫瘍に対する直流通電療法の抗腫瘍効果 (2013)
  • Author(s): 林克洋, 松下廉, 山田聡, 稲谷弘幸, 立松尚衞, 岡本秀貴, 土屋弘行, 大塚隆信
  • Organizer: 第40回日本生体電気・物理刺激研究会
  • Place of Presentation: ウエスティン都ホテル(京都府)
• [Presentation] 15年以上経過し筋肉内転移が診断された腎細胞癌の2例 (2013)
  • Author(s): 林克洋, 村瀬熱紀, 山田聡, 稲谷弘幸, 岡本秀貴, 大塚隆信, 土屋弘行
  • Organizer: 第32回日本画像医学会
  • Place of Presentation: 東京ステーションコンファレンス(東京都)
• [Presentation] Leading Technology for in vivo Fluorescent Sarcoma Imaging (2013)
  • Author(s): Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Hoffman RM, Otsuka T.
  • Organizer: European Musculo Skeletal Oncology Society
  • Place of Presentation: Swedish Exhibition and Congress Center (Gothenburg,Sweden)
• [Presentation] Long term result of “second-look operation” following unplanned excision of soft tissue malignancy (2013)
  • Author(s): Hayashi K, Yamada S, Inatani H, Okamoto H, Tsuchiya H, Otsuka T.
  • Organizer: International Society of Limb Salvage
  • Place of Presentation: Palazzo Re Enzo (Bologna,Italy)
• [Presentation] Biological reconstruction after excision of bone metastasis using tumor-bearing autografts treated with liquid nitrogen (2013)
  • Author(s): Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Tanazawa Y, Takeuchi A, Inatani H, Otsuka T.
  • Organizer: Hong Kong Orthpedic Association Annual Congress
  • Place of Presentation: 香港会議展覧中心(Hong Kong,中国)
• [Presentation] Long-term histological findings of oncologically sterilized autoclaved bone, irradiated bone and frozen bone (2013)
  • Author(s): Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Tanazawa Y,
  • Organizer: 低温医学学会総会
  • Place of Presentation: 今池ガスビル（名古屋）
• [Presentation] 骨軟部悪性腫瘍に対する直流通電療法の抗腫瘍効果 (2013)
  • Author(s): 林克洋 松下廉 山田聡 稲谷弘幸 立松尚衞 岡本秀貴 土屋弘行 大塚隆信
  • Organizer: 第40回日本生体電気・物理刺激研究会
  • Place of Presentation: ウエスティン都ホテル (京都府）
• [Presentation] 15年以上経過し筋肉内転移が診断された腎細胞癌の2例 (2013)
  • Author(s): 林克洋 村瀬熱紀 山田聡 稲谷弘幸 岡本秀貴 大塚隆信 土屋弘行
  • Organizer: 第32回日本画像医学会
  • Place of Presentation: 東京ステーションコンファレンス (東京都）
• [Presentation] Comparison of Sarcoma Cell Seeding in the Lung and Muscle, Visualized by Real-Time Imaging in the Live Mouse (2012)
  • Author(s): Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Hoffman RM
  • Organizer: AAOS American Academy of Orthopaedic Surgeons
  • Place of Presentation: Moscone center (USA)
  • Year and Date: 2012-02-09
• [Presentation] 転移性骨腫瘍の切除後に液体窒素処理した腫瘍骨を用いた生物学的再建 (2012)
  • Author(s): 林克洋、土屋弘行、山本憲男、白井寿治、西田英司、丹沢義一、武内章彦、木村浩明、三輪真嗣、五十嵐健太郎、稲谷弘幸、下崎真吾、大塚隆信
  • Organizer: 日本低温医学会総会
  • Place of Presentation: イイノコンファレンスセンター(東京都)
• [Presentation] 低悪性度の骨肉腫の診断と治療について (2012)
  • Author(s): 林克洋、大塚隆信、土屋弘行
  • Organizer: 中部整形外科災害外科学会
  • Place of Presentation: フェニックスプラザ(福井県)
• [Presentation] Multicenter Study of Caffeine-potentiated Chemotherapy for Bone and Soft Tissue Sarcoma (2012)
  • Author(s): Hayashi K, Tsuchiya H, Takeuchi A, Otsuka T, Hoshi M, Ieguchi M, Chosa E, Sakamoto T, Tajino T, Yamada H, Yokouchi M, Sakayama K, Kidani T, Isobe K, Maehara H, Tome Y
  • Organizer: 9th Asia Pacific Musculoskeletal Tumor Society Meeting (APMSTS)
  • Place of Presentation: Hilton Kuala Lumpur (Malaysia)
• [Presentation] Multicenter Study of Caffeine-potentiated Chemotherapy for Bone and Soft Tissue Sarcoma (2012)
  • Author(s): Hayashi K, Tsuchiya H, Takeuchi A, Otsuka T, Hoshi M, Ieguchi M, Chosa E, Sakamoto T, Tajino T, Yamada H, Yokouchi M, Sakayama K, Kidani T, Isobe K, Maehara H, Tome Y
  • Organizer: 9th Asia Pacific Musculoskeletal Tumor Society Meeting
  • Place of Presentation: Hilton Kuala Lumpur (Malaysia)
• [Presentation] 転移性骨腫瘍の切除後に液体窒素処理した腫瘍骨を用いた生物学的再建 (2012)
  • Author(s): 林克洋、土屋弘行、山本憲男、白井寿治、西田英司、丹沢義一、武内章彦、木村浩明、三輪真嗣、五十嵐健太郎、稲谷弘幸、下崎真吾、大塚隆信
  • Organizer: 日本低温医学会総会
  • Place of Presentation: イイノコンファレンスセンター（東京都）
• [Presentation] 低悪性度の骨肉腫の診断と治療について (2012)
  • Author(s): 林克洋、大塚隆信、土屋弘行
  • Organizer: 中部整形外科災害外科学会
  • Place of Presentation: フェニックスプラザ（福井県）
• [Presentation] Comparison of Sarcoma Cell Seeding in the Lung and Muscle, Visualized by Real-Time Imaging in the Live Mouse (2012)
  • Author(s): Hayashi K
  • Organizer: 2012 Anual Meeting of The American Academy of Orthopaedic Surgeons
  • Place of Presentation: Moscone center(USA)
• [Presentation] シンポジウム「腫瘍の転移」肉腫細胞の可視化モデルを用いた肉腫転移機構の解明 (2011)
  • Author(s): 林克洋,土屋弘行,山本憲男,白井寿治,西田英司,丹沢義一,武内章彦,木村浩明三輪真嗣,五十嵐健太郎,ロバートMホフマン
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: ベイシア文化ホール(群馬県)
  • Year and Date: 2011-10-20
• [Presentation] Comparison of Sarcoma Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-Time Imaging in the Live Mouse (2011)
  • Author(s): Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Tanzawa Y, Takeuchi A, Kimura H, Miwa S, Igarashi K, Hoffman RM
  • Organizer: International Society of Limb Salvage/Musculoskeletal Tumor Society Combined Meeting
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2011-09-17
• [Presentation] 肉腫細胞の可視化モデルを用いた肉腫転移機構の解明 (2011)
  • Author(s): 林克洋
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: ベイシア文化ホール(群馬県)