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Direct transplantation of mesenchymal stem cells into the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis研究代表者

Research Project

Project/Area Number 23791632
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionUniversity of Fukui

Principal Investigator

MIYAZAKI Tsuyoshi  福井大学, 医学部附属病院, 助教 (80324169)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords骨 / 軟骨代謝学 / 幹細胞 / 軟骨再生 / 変形性関節症
Research Abstract

Mesenchymal stem cells (MSCs) can differentiate into various connective tissue cells. Several techniques have been used for the clinical application of MSCs in articular cartilage repair; however, there are many issues associated with the selection of the scaffold material, including its ability to support cell viability and differentiation and its retention and degradation in situ. The application of MSCs via a scaffold also requires a technically demanding surgical procedure. The aim of this study was to test the outcome of intra-articular transplantation of mesenchymal stem cells suspended in hyaluronic acid (HA) in the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis (OA). At 5 weeks post transplantation, partial cartilage repair was noted in the HA-MSC group but not in the other groups. Examination of CFDA-SE-labeled cells demonstrated migration, differentiation, and proliferation of MSC in the HA-MSC group. There was strong immunostaining for type II collagen around both residual chondrocytes and transplanted MSCs in the OA cartilage. Conclusion: This scaffold-free and technically undemanding technique appears to result in the regeneration of articular cartilage in the spontaneous OA animal model. Although further examination of the long-term effects of transplantation is necessary, the findings suggest that intra-articular injection of HA-MSC mixture is potentially beneficial for OA.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (2 results)

All 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results)

  • [Journal Article] Direct transplantation of mesenchymal stem cells into the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis2013

    • Author(s)
      Sato Mitsuhiko
    • Journal Title

      Arthritis Research & Therapy

      Volume: 7;14(1) Issue: 1

    • DOI

      10.1186/ar3735

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alexander Rodriguez Guerrero, Shuji Watanabe, Sally Roberts, Hisatoshi Baba. Direct transplantation of mesenchymal stem cells into the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis2012

    • Author(s)
      Mitsuhiko Sato, Kenzo Uchida, Hideaki Nakajima, Tsuyoshi Miyazaki
    • Journal Title

      Arthritis Research & Therapy

      Volume: 14(1) Pages: 7-7

    • Related Report
      2012 Final Research Report
    • Peer Reviewed

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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