The mechanisms of induction of autophagic cell death by histone deacetylase inhibitor SAHA

• Project/Area Number: 23791660
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kyushu University
• Principal Investigator: OKADA Takamitsu 九州大学, 大学病院, 助教 (70525550)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: autophagic cell death

Research Abstract

In electron microscope SAHA induced autophagosome in multidrug-resistant osteosarcoma cell. SAHA also induced autophagy related protein Beclin1 and LC3-I 、II in dose dependent manner. These facts demonstrated that SAHA induce autophagic cell death in multidrug-resistant osteosarcoma cell. Next, we investigated cell cycle population in osteosarcoma parental cell and multidrug-resistant cell after SAHA treatment. SAHA increased Sub-G1 fraction in parental cell, but G2/M fraction in multidrug-resistant cell. Therefore, we investigated the relationship between autopphagy and G2/M phase. As a result, we got new fact that SAHA induce autophagy and G2/M phase at the same time