Monitoring of neuroinflammation and creation of new strategy after spinal cord injury.

• Project/Area Number: 23791665
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Showa University
• Principal Investigator: SATO Atsushi 昭和大学, 医学部, 助教 (10445596)
• Research Collaborator: OHTAKI Hirokazu 昭和大学, 助教 (20349062) ; SHIODA Seiji 昭和大学, 教授 (80102375)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 脊髄損傷 / 神経炎症 / マイクログリア / マクロファージ / インターロイキン１ / 骨髄間葉系幹細胞 / 代替経路型活性化型 / 古典的活性化型 / ヒト骨髄間葉系幹細胞 / PACAP / IL-1 / 炎症応答

Research Abstract

Microglia and macrophages (MG/MF) have a diverse range of functions depending on unique cytokine stimuli, and contribute to neural cell death, repair, and remodeling during central nervous system diseases. While IL-1 has been shown to exacerbate inflammation, it has also been recognized to enhance neurodegeneration. We determined the activating phenotype of MG/MF and the impact of IL-1 in an in vivo spinal cord injury (SCI) model of IL-1 knock-out (KO) mice. Moreover, we demonstrated the contribution of IL-1 to both the classical and alternative activation of MG in vitro using an adult MG primary culture.
We demonstrate here in in vivo experiments that IL-1 suppressed SCI in a process mediated by the reduction of inflammatory responses. Moreover, we suggest that IL-1 participates in both the classical and alternative activation of MG in in vivo and in vitro systems. hMSCs Implanted to injured spinal cord have been recognized to improve motor function, neuroinflammation and regeneration.

Research Products

• [Journal Article] Establishment and Characterization of Primary Adult Microglial Culture in Mice (2013)
  • Author(s): Ohtaki H, Tsumuraya T, Song D, Sato A, Ohara K, Miyamoto K, Nakano H, Kiriyama K, Dohi K, Hiraizumi H, Matsunaga M, Shioda S
  • Journal Title: Acta Neurochir Suppl Volume: 118 Pages: 49-54
  • DOI: 10.1007/978-3-7091-1434-6_8
  • ISBN: 9783709114339, 9783709114346
  • Peer Reviewed
• [Journal Article] Establishment and characterization of primary adult microglial culture in mice (2013)
  • Author(s): Ohtaki H, Tsumuraya T, Song D, Sato A, Ohara K, Miyamoto K, Nakano H, Kiriyama K, Dohi K, Hiraizumi Y, Matsunaga M, Shioda S
  • Journal Title: Acta Neurochirurgica Suppl Volume: in press
• [Journal Article] Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury (2012)
  • Author(s): Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S
  • Journal Title: J Neuroinflammation Volume: 9 Issue: 1 Pages: 65-65
  • DOI: 10.1186/1742-2094-9-65
  • Peer Reviewed
• [Presentation] 脊髄損傷マウスにおけるマイクログリア/マクロファージ活性化へのインターロイキン1の関与 (2013)
  • Author(s): 佐藤敦,大滝博和,小原賢司,圓谷智海,平泉裕,渥美敬,塩田清二
  • Organizer: 第86回日本整形外科学会学術総会
  • Place of Presentation: 広島グリーンアリーナ(広島・広島)
• [Presentation] Stem cell characteristics and anti-inflammation properties of hMSCs in different passages (2013)
  • Author(s): Dandan Song, Hirokazu Ohtaki, Tomomi Tsumuraya, Atsushi Sato, Yutaka Hiraizumi,Tomio Inoue, Seiji Shioda
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポート高松・かがわ国際会議場(高松・香川)
• [Presentation] Human stem/progenitor cells from bone marrow improve spinal cord injury via communicating with PACAP (2013)
  • Author(s): Ohtaki H, Tsumuraya T, Song D, Sato A, Xu Z, Miyamoto K, Hiraizumi Y, Nakamachi T, Hashimoto H, Shintani N, Shioda S
  • Organizer: The 11th International Symposium on VIP, PACAP and Related Peptides
  • Place of Presentation: Hungary University of Pecs Szentagothai Research Centre (Pecs, Hungary)
• [Presentation] 脊髄損傷マウスにおけるマイクログリア／マクロファージ活性化へのインターロイキン１の関与 (2013)
  • Author(s): 佐藤敦，大滝博和，小原賢司，圓谷智海，平泉裕，渥美敬，塩田清二
  • Organizer: 第86回日本整形外科学会学術総会
  • Place of Presentation: 広島・広島
• [Presentation] Human stem/progenitor cells from bone marrow improve spinal cord injury via communicating with PACAP. (2013)
  • Author(s): Ohtaki H, Tsumuraya T, Song D, Sato A, Xu Z, Miyamoto K, Hiraizumi Y, Nakamachi T, Hashimoto H, Shintani N, Shioda S
  • Organizer: The 11th International Symposium on VIP, PACAP and Related Peptides
  • Place of Presentation: Pecs, Hungary University of Pecs Szentagothai Research Centre
• [Presentation] Stem cell characteristics and anti-inflammation properties of hMSCs in different passages (2013)
  • Author(s): Dandan Song, Hirokazu Ohtaki, Tomomi Tsumuraya, Atsushi Sato, Yutaka Hiraizumi,Tomio Inoue, Seiji Shioda
  • Organizer: 第１１８回日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松・香川
• [Presentation] Interleukin-1 (IL-1) may contribute to develop classical and alternative activation of microglia/macrophages after spinal cord injury (2012)
  • Author(s): Hirokazu Ohtaki, Atsushi Sato, Tomomi Tsumuraya, Dandan Song, Kazuyuki Miyamoto, Kenji Ohara, Masahide Asano, Yoichiro Iwakura, Takashi Atsumi, Seiji Shioda
  • Organizer: 11th International Society of NeuroImmunology Congress
  • Place of Presentation: he Westin Copley Place Boston (Boston, MA)
• [Presentation] Interleukin-1 (IL-1) may contribute to develop classical and alternative activation of microglia/macrophages after spinal cord injury (2012)
  • Author(s): Hirokazu Ohtaki, Atsushi Sato, Tomomi Tsumuraya, Dandan Song, Kazuyuki Miyamoto, Kenji Ohara, Masahide Asano, Yoichiro Iwakura, Takashi Atsumi, Seiji Shioda
  • Organizer: 11th International Society of NeuroImmunology Congress
  • Place of Presentation: Boston, MA