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Induction of Mesenchymal Stem Cells from Pluripotent Stem Cells

Research Project

Project/Area Number 23791670
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionKinki University

Principal Investigator

TERAMURA Takeshi  近畿大学, 医学部附属病院, 講師 (40460901)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords再生医療 / 多能性幹細胞 / 間葉系幹細胞 / 軟骨再生 / 分化誘導 / ES細胞 / iPS細胞
Research Abstract

In this study, we derived the MSCs from rabbit ESCs and human iPSCs both in vivo and in vitro, and examined their detailed characteristics.
Both in vivo- and in vitro-pcMSCs expressed MSC-markers and showed multiple differentiation properties. In the cases of the in vitro-pcMSCs, teratoma formations were not observed, and when rabbit ESC-derived in vitro-pcMSCs were transplanted into the model rabbits, successful engraftment were observed. On the other hand, the in vivo-induced MSCs derived from human iPSCs showed high telomerase activity and formed teratomas.
We could obtain the MSC-like cells both in vivo and in vitro. However, the present results suggest that pluripotent stem cells could derive various types of MSCs, and we should select the cells carefully based on the appropriate markers for our purposes.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2013 2012 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (7 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Generation of embryonic stem cell lines from immature rabbit ovarian follicles2013

    • Author(s)
      Teramura T, Sugimoto H, Frampton J, Kida Y, Nakano M, Kawakami M, Izumi H, Fukunaga N, Onodera Y, Takehara T, Fukuda K, Hosoi Y
    • Journal Title

      Stem Cells Dev

      Volume: 22(6) Pages: 928-38

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of functional mesenchymal stem cells from rabbit embryonic stem cells by exposure to severe hypoxic conditions2013

    • Author(s)
      Teramura T, Onodera Y, Takehara T, Frampton J, Matsuoka T, Ito S, Nakagawa K, Miki Y, Hosoi Y, Hamanishi C, Fukuda K
    • Journal Title

      Cell Transplant

      Volume: 22(2) Pages: 309-29

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induced Pluripotent Stem Cells in Reproductive Medicine2013

    • Author(s)
      Teramura T et al.
    • Journal Title

      Reproductive Medicine and Biology

      Volume: 12 Issue: 2 Pages: 39-46

    • DOI

      10.1007/s12522-012-0141-x

    • NAID

      10031165252

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation of Embryonic Stem Cell Lines from Immature Rabbit Ovarian Follicles2013

    • Author(s)
      Teramura T et al.
    • Journal Title

      Stem Cells and Development

      Volume: 15 Issue: 6 Pages: 928-938

    • DOI

      10.1089/scd.2012.0300

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells2012

    • Author(s)
      Takehara T, Teramura T, Onodera Y, Hamanishi C, Fukuda K
    • Journal Title

      Stem Cells Dev

      Volume: 21(8) Pages: 1239-49

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical stimulation of cyclic tensile strain induces reduction of pluripotent related gene expressions via activation of Rho/ROCK and subsequent decreasing of AKT phosphorylation in human induced pluripotent stem cells2012

    • Author(s)
      Teramura T, Takehara T, Onodera Y, Nakagawa K, Hamanishi C, Fukuda K
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 417(2) Pages: 836-41

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical stimulation of cyclic tensile strain2012

    • Author(s)
      Takeshi Teramura et al.
    • Journal Title

      Biochem Biophys Res Comm

      Volume: 417 Issue: 2 Pages: 836-841

    • DOI

      10.1016/j.bbrc.2011.12.052

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Induction of Mesenchymal Stem Cells from Human iPS cells in vivo and in vitro2013

    • Author(s)
      Takeshi Teramura, Toshiyuki Takehara, Yuta Onodera, John Frampton, Kanji Fukuda
    • Organizer
      11^<th> International Society for Stem Cell Research, Annual Meeting
    • Place of Presentation
      Boston,MA USA
    • Year and Date
      2013-06-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 低酸素培養によるヒトiPS細胞からの間葉系幹細胞の分化誘導2013

    • Author(s)
      寺村岳士、小野寺勇太、竹原俊幸、福田寛二
    • Organizer
      第12回日本再生医療学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 間葉系幹細胞シートによるラット関節軟骨全層欠損の軟骨再生2013

    • Author(s)
      寺村岳士、小野寺勇太、竹原俊幸、福田寛二
    • Organizer
      第26回日本軟骨代謝学会
    • Place of Presentation
      千里ライフサイエンスセンター
    • Year and Date
      2013-03-01
    • Related Report
      2013 Final Research Report
  • [Presentation] Generation and Characterization of Mesenchymal Stem Cells from Human iPSCs in vivo and in vitro2013

    • Author(s)
      Takeshi Teramura
    • Organizer
      第11回International Society for Stem Cell Research
    • Place of Presentation
      ボストン(米国)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 低酸素培養によるウサギES細胞からの間葉系幹細胞の分化誘導2012

    • Author(s)
      寺村岳士、小野寺勇太、竹原俊幸、中川晃一、浜西千秋、福田寛二
    • Organizer
      第11回日本再生医療学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2012-06-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 低酸素培養によるヒトiPS細胞からの間葉系幹細胞の分化誘導

    • Author(s)
      寺村 岳士 他3名
    • Organizer
      日本再生医療学会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒトiPS細胞からの間葉系幹細胞の分化誘導

    • Author(s)
      寺村岳士
    • Organizer
      軟骨代謝学会
    • Place of Presentation
      ウィルあいち(名古屋市)
    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] 免疫不全動物を用いた細胞の製法2010

    • Inventor(s)
      寺村岳士、福田寛二
    • Industrial Property Rights Holder
      学校法人近畿大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2010-12-08
    • Related Report
      2013 Final Research Report
    • Overseas

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Published: 2011-08-05   Modified: 2019-07-29  

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