| Project/Area Number |
23791685
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Gunma University |
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Principal Investigator |
KUBO kazuhiro 群馬大学, 医学(系)研究科(研究院), 助教 (80546531)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 麻酔薬 / GAD65 / GAD67 / VGAT / GFP / 吸入麻酔薬 |
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| Research Abstract |
All procedures were performed on adult (12-16 weeks of age) male wild-type(WT) and GAD65 knockout mice (GAD65-/-).Three pain-related behavior tests(Hotplate test; Tail-immersion test; Formaline test) were performed to assess response to nociceptive stimulation. We measured latencies to licking or jumping from the hotplate set at53centigrade A significant alteration of the response was found in male GAD65 (-/-) (p<0.01)compared to their wild-type controls.indicative of increased nociceptive perception. However, there was no significant difference in the latency of the tail-immersion test, which was known to spinally-mediated nociceptive thresholds. In formalin test,there were no significant difference in peak response time of phaseI and phaseII,but GAD65(-/-)mice indicate the prolongation of hepersensitivity in phaseII
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