| Project/Area Number |
23791691
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | University of Fukui |
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Principal Investigator |
MATSUKI Yuka 福井大学, 医学部, 助教 (10464083)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 麻酔学 / チャネル / カリウムチャネル / 麻酔薬 / 脂質平面膜 |
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| Research Abstract |
We prepared E71A mutant of KcsA channel. Channels were reconstruct into the planar lipid bilayer membrane and single-channel current were recorded. The Popenwas plotted against pH and was fitted with the Hill equation, yielding a pKa of 5.2 and nH = 2.2. Open and closed dwell-time distributions of the E71A mutant at various pH conditions. Data were obtained at pH 4.0, pH 5.0, and pH 5.5 for the E71A mutant, respectively. The slowest component of the closed dwell-time appeared as the pH increased. Next, I tried to establish the planar lipid membrane method using a volatileanesthesia. After embedding potassium channels in the planar lipid membrane andwas subjected to a current recording potassium channels, it was completely sealed by using an acrylic plate.
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