Research on descending pain control mechanism

• Project/Area Number: 23791693
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Shinshu University
• Principal Investigator: SUGIYAMA Daisuke 信州大学, 医学部附属病院, 特任研究員 (40467189)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 下行性抑制系 / ノルアドレナリン / 青斑核 / 下行性痛み抑制系 / in vivoパッチクランプ

Research Abstract

To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potentialoscillations. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.

Research Products

• [Journal Article] 下行性ノルアドレナリン痛覚抑制機構とその活動制御 (2012)
  • Author(s): 古江秀昌、井本 敬二、 杉山 大介、 川真田 樹人、 舟井 優介、 西川 精宣
  • Journal Title: 麻酔 Volume: 61巻増刊
  • Peer Reviewed
• [Journal Article] In vivo patch-clamp recording from locus coeruleus neurons in the rat brainstem (2012)
  • Author(s): Sugiyama D, Hur SW, Pickering AE, Kase D, Kim SJ, Kawamata M, Imoto K, Furue H
  • Journal Title: Journal of Physiology Volume: (in press) Issue: 10 Pages: 2225-2231
  • DOI: 10.1113/jphysiol.2011.226407
  • NAID: 120007108244
  • Peer Reviewed
• [Journal Article] 下行性ノルアドレナリン痛覚抑制機構とその活動制御 (2012)
  • Author(s): 古江秀昌，井本敬二，杉山大介，他
  • Journal Title: 麻酔 Volume: 61増刊
  • Peer Reviewed
• [Presentation] ラット脳幹青斑核からの in vivo パッチクランプ記録 (2012)
  • Author(s): 杉山大介、 井本敬二、 川真田樹人、 古江秀昌
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
• [Presentation] In vivo whole-cell patch-clamp analysis of synaptic responses evoked in locus coeruleus neurons in the rat brainstem (2011)
  • Author(s): Daisuke Sugiyama, Keiji Imoto, A.E. Pickering, Mikito Kawamata, Hidemasa Furue
  • Organizer: Annual Meeting of Society for Neuroscience
  • Place of Presentation: WashintonDC, USA
• [Presentation] In vivo whole-cell patch-clamp analysis of synaptic responses evoked in locus coeruleus neurons in the rat brainstem (2011)
  • Author(s): 杉山大介
  • Organizer: Annual Meeting of Society for Neuroscience
  • Place of Presentation: 米国（ワシントンD.C.）