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The elucidation of ATP releasing pathway of bladder epithelial cell upon stretch stimulation

Research Project

Project/Area Number 23791749
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Urology
Research InstitutionUniversity of Yamanashi

Principal Investigator

NAKAGOMI Hiroshi  山梨大学, 医学部附属病院, 助教 (80418714)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsシグナル伝達 / 生理学 / 神経科学 / 国際情報交換 / 国際情報交流
Research Abstract

We have investigated exocytotic release of ATP from urothelium, especially focusing on vesicular nucleotide transporter (VNUT). VNUT gene and protein was highly expressed in the epithelial layer of mouse bladder tissue and primary urothelial cell culture. The stretch-evoked ATP release was significantly reduced by the treatment of VNUT siRNA or several inhibitors of vesicular exocytosis. These findings suggest that VNUT plays an important role in stretch-elicited ATP release from urothelium.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (31 results)

All 2013 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 1 results) Presentation (24 results) Remarks (1 results)

  • [Journal Article] 医薬ジャーナル2013

    • Author(s)
      武田正之、荒木勇雄、澤田智史、中込宙史、望月勉、小林英樹、座光寺秀典、芳山充晴
    • Journal Title

      新薬展望 2013

      Volume: Vol. 49

    • Related Report
      2012 Final Research Report
  • [Journal Article] 排尿障害におけるβ3 受容体刺激(作 動)薬2012

    • Author(s)
      武田正之、中込宙史、芳山充晴、荒木勇雄
    • Journal Title

      診断と治療

      Volume: 第100巻・第8号 Pages: 1379-1385

    • Related Report
      2012 Final Research Report
  • [Journal Article] 排尿障害におけるβ3 受容体作動薬2012

    • Author(s)
      武田正之、中込宙史、芳山充晴、荒木勇雄
    • Journal Title

      Progress in Medicine

      Volume: Vol. 32 (No. 4) Pages: 37-42

    • Related Report
      2012 Final Research Report
  • [Journal Article] 第4回国際尿失禁会議からの報告2011

    • Author(s)
      武田正之、中込宙史、宮本達也、吉良聡、芳山充晴
    • Journal Title

      Committee3 Cell Biology 「細胞生物学」.排尿障害プラクティス

      Volume: Vol.19(1) Pages: 66-88

    • Related Report
      2012 Final Research Report
  • [Journal Article] Sensor mechanism and afferent signal transduction of the urinary bladder: special focus on transient receptor potential ion channels2010

    • Author(s)
      Takeda M, Mochizuki T, Yoshiyama M, Nakagomi H, Kobayashi H, Sawada N, Zakohji H, Du S, Araki I
    • Journal Title

      LUTS (Lower Urinary Tract Symptoms)

      Volume: 2 Pages: 51-60

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 尿路上皮と排尿2010

    • Author(s)
      荒木勇雄、中込宙史、望月勉、小林英樹、芳山充晴、武田正之
    • Journal Title

      臨床検査

      Volume: Vol.54(7) Pages: 726-730

    • Related Report
      2012 Final Research Report
  • [Presentation] VNUT plays a crucial role in stretch-evoked ATP releasefrom urothelium2013

    • Author(s)
      中込 宙史
    • Organizer
      アメリカ泌尿器科学会
    • Place of Presentation
      Georgia World Congress Center(Atlanta USA)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト膀胱上皮における VNUT m-RNA発現量と各種排尿パラメータとの関連性2013

    • Author(s)
      中込 宙史
    • Organizer
      日本泌尿器科学会総会
    • Place of Presentation
      さっぽろ芸術文化の館(北海道)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 代謝型グルタミン酸受容体サブタイプ5 を介する神経伝達の遮断は膀胱収縮性及び排尿を損ねずに蓄尿を促す2012

    • Author(s)
      芳山充晴、土屋幸子、望月勉、中込宙史、宮本達也、吉良聡、武田正之
    • Organizer
      第19回日本排尿機能学会
    • Place of Presentation
      愛知県名古屋市・名古屋国際会議場
    • Year and Date
      2012-08-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Vesicular nucleotide transporter (VNUT) is a key molecule for mechanosensing in the human urinary bladder: negative correlation between VNUT expression in human bladder mucosa and first desire to void2012

    • Author(s)
      Nakagomi H, Mochizuki T, Miyamoto T, Kira S, Yoshiyama M, Kawahara K, Moriyama Y, Koizumi S, Takeda M
    • Organizer
      (Abstract No. 12) International Continence Society 42nd Annual meeting, China National Convention Centre
    • Place of Presentation
      Beijing, China
    • Related Report
      2012 Final Research Report
  • [Presentation] VNUT (vesicular nucleotide transporter) plays a crucial role in stretch-evoked ATP release from urothelium2012

    • Author(s)
      Nakagomi H, Mochizuki T, Miyamoto T, Yoshiyama M, Araki I, Moriyama Y, Koizumi S, Takeda M
    • Organizer
      (Abstract No. 495, May 20, 2012) American Urological Association Annual Meeting, Georgia World Congress Center
    • Place of Presentation
      Atlanta, Georgia, U.S.A
    • Related Report
      2012 Final Research Report
  • [Presentation] Evaluation of increased voiding frequency in mice with type 2 diabetes mellitus2012

    • Author(s)
      Yoshiyama M, Mochizuki T, Nakagomi H, Miyamoto T, Takeda M
    • Organizer
      (Abstract No. 256, May 20, 2012) American Urological Association Annual Meeting
    • Place of Presentation
      Georgia World Congress Center, Atlanta, Georgia, U.S.A.
    • Related Report
      2012 Final Research Report
  • [Presentation] 伸展刺激時の膀胱上皮からのATP放出機構におけるVNUT/開口放出経路の解析2012

    • Author(s)
      中込 宙史
    • Organizer
      日本泌尿器科学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Related Report
      2012 Annual Research Report
  • [Presentation] The relations between the expression Vesicular Nucleotide Transporter ( VNUT ) in the human bladder mucosa and urological clinical parameters.2012

    • Author(s)
      武田 正之
    • Organizer
      国際尿禁制学会
    • Place of Presentation
      中国国際会議場(北京 中国)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 伸展刺激に対する膀胱上皮細胞からのATP放出メカニズム2012

    • Author(s)
      中込宙史
    • Organizer
      第85回日本薬理学会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 新規のマウス用排尿代謝ケージ構築ならびに各種パラメーターについて~野生型マウスとTRPV4 ノックアウトマウスとの比較~2011

    • Author(s)
      望月勉,中込宙史,芳山充晴,高山靖規,富永真琴,水町凉治,武田正之
    • Organizer
      第18回日本排尿機能学会
    • Place of Presentation
      福井県福井市
    • Related Report
      2012 Final Research Report
  • [Presentation] 遺伝性発症2 型糖尿病マウスの若年期における頻尿の病態解析2011

    • Author(s)
      芳山充晴,望月勉,中込宙史,宮本達也,武田正之
    • Organizer
      第18回日本排尿機能学会
    • Place of Presentation
      福井県福井市
    • Related Report
      2012 Final Research Report
  • [Presentation] VNUT (Vesicular Nucleotide Transporter) を介する膀胱上皮からの ATP 放出メカニズムの解明2011

    • Author(s)
      中込宙史,望月勉,芳山充晴,宮本達也,吉良聡,川原和也,荒木勇雄,小泉修一,森山芳則,武田正之
    • Organizer
      第18回日本排尿機能学会
    • Place of Presentation
      福井県福井市
    • Related Report
      2012 Final Research Report
  • [Presentation] Comparisons between voluntary behaviour and reflex response in control of the lower urinary tract function of mice2011

    • Author(s)
      Yoshiyama M, Mochizuki T, Nakagomi H, Kobayashi H, Miyamoto T, Zakoji H, Takeda M
    • Organizer
      (Abstract No. 453) International Continence Society Meeting 41st
    • Place of Presentation
      Glasgow, U.K.
    • Related Report
      2012 Final Research Report
  • [Presentation] TRPV4-deficient mice exhibit increased frequency of voiding ~evaluation of micturition behaviour using novel metabolic cage system2011

    • Author(s)
      Mochizuki T, Yoshiyama M, Nakagomi H, Miyamoto T, Takeda M
    • Organizer
      (Abstract No. 411) International Continence Society Meeting 41st
    • Place of Presentation
      Glasgow, U.K.
    • Related Report
      2012 Final Research Report
  • [Presentation] Risk factors of nocturia and assessment of nocturia-related quality of life in Japanese population2011

    • Author(s)
      Imamura N, Yoda Y, Nakagomi H, Miyamoto T, Tsuchida T, Fukazawa M, Araki I, Yoshiyama M, Takeda M
    • Organizer
      (Abstract No. 375) International Continence Society Meeting 41st
    • Place of Presentation
      Glasgow, U.K.
    • Related Report
      2012 Final Research Report
  • [Presentation] VNUT plays an important role in vesicular storage and subsequent exocytosis of ATP from bladder epithelium upon mechanical stretch stimulation2011

    • Author(s)
      Nakagomi H, Mochizuki T, Miyamoto T, Kira S, Yoshiyama M, Araki I, Koizumi S, Moriyama Y, Takeda M
    • Organizer
      (Abstract No. 278) International Continence Society Meeting 41st
    • Place of Presentation
      Glasgow, U.K.
    • Related Report
      2012 Final Research Report
  • [Presentation] Evalutations of increased voiding frequency in mice with diabetes mellitus at early stage2011

    • Author(s)
      Yoshiyama M, Mochizuki T, Nakagomi H, Miyamoto T, Kobayashi H, Zakoji H, Takeda M
    • Organizer
      (Abstract No. 182) International Continence Society Meeting 41st
    • Place of Presentation
      Glasgow, U.K.
    • Related Report
      2012 Final Research Report
  • [Presentation] 膀胱上皮細胞の伸展刺激に対するATP放出メカニズムの解明2011

    • Author(s)
      中込宙史
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Related Report
      2011 Research-status Report
  • [Presentation] 膀胱上皮のTRPVチャネルとATP放出メカニズム2011

    • Author(s)
      中込宙史
    • Organizer
      第99回日本泌尿器科学会総会(招待講演)
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Related Report
      2011 Research-status Report
  • [Presentation] VNUT(Vesicular Nucleotide Transporter)を介する膀胱上皮からのATP放出メカニズムの解明2011

    • Author(s)
      中込宙史
    • Organizer
      第18回日本排尿機能学会
    • Place of Presentation
      福井フェニックスプラザ(福井)
    • Related Report
      2011 Research-status Report
  • [Presentation] VNUT PLAYS AN IMPORTANT ROLE IN VESICULAR STORAGE AND SUBSEQUENT EXOCYTOSIS OF ATP FROM BLADDER EPITHELIUM UPON MECHANICAL STRETCH STIMULATION2011

    • Author(s)
      Hiroshi Nakagomi
    • Organizer
      41st ANNUAL MEETING OF THE INTERNATIONAL CONTINENCE SOCIETY (ICS)
    • Place of Presentation
      ScotishiExhibition&conference Center(Glasgow,UK)
    • Related Report
      2011 Research-status Report
  • [Presentation] 新規ディバイス排尿用代謝ケージを用いたマウス排尿測定~野生型マウスの各種排尿パラメーターにおける雌雄差について2010

    • Author(s)
      望月勉,中込宙史,芳山充晴,水町凉治,武田正之
    • Organizer
      第17回日本排尿機能学会
    • Place of Presentation
      山梨県甲府市
    • Related Report
      2012 Final Research Report
  • [Presentation] Gene expression patterns of acid-sensing ion channels (ASICs) families in mammalian bladder2010

    • Author(s)
      Kobayashi H, Yoshiyama M, Inuzuka H, Kira S, Nakagomi H, Miyamoto T, Haneda Y, Sawada N, Zakoji H, Tsuchida T, Araki I, Takeda M
    • Organizer
      (Abstract No. 520) International Continence Society 40th/International Uro-Gynecology Association Joint Meeting
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] Hypertension and type 2 diabetes mellitus are highly associated with nocturia than metabolic syndrome in Japanese population2010

    • Author(s)
      Imamura N, Nakagomi H, Yoda Y, Zakoji H, Kobayashi H, Tsuchida T, Fukazawa M, Yoshiyama M, Araki I, Takeda M
    • Organizer
      (Abstract No. 381) International Continence Society 40th /International Uro-Gynecology Association Joint Meeting
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2012 Final Research Report
  • [Remarks] なし

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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