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Characterization of the low pH-resistant LNCaP cell subline

Research Project

Project/Area Number 23791768
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Urology
Research InstitutionGifu Pharmaceutical University

Principal Investigator

IGUCHI Kazuhiro  岐阜薬科大学, 薬学部, 講師 (10295545)

Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
KeywordsLNCaP細胞 / LNCaP-F10細胞 / 細胞外低pH / アポトーシス / survivin / caspase-10 / TNFSF10 / TRAIL / ビカルタミド / アンドロゲン受容体 / LNCaP / LNCaP-F10
Outline of Final Research Achievements

Intratumoral region of low extracellular pH is common feature of solid tumors. The subline LNCaP-F10, of the prostate cancer cell line LNCaP, was isolated and its low pH-resistant property was examined. LNCaP-F10 cells were grown under low-pH condition, which caused apoptosis of the LNCaP cells. Significant differences in the expression of TNFSF10, BIRC5 and CASP10 were detected between LNCaP-F10 and LNCaP cells. These factors were related with the apoptosis induction by extracellular low pH.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (8 results)

All 2014 2013 2012 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results)

  • [Journal Article] Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10.2012

    • Author(s)
      Iguchi K, Hayakawa Y, Ishii K, Matsumoto K, Usui S, Sugimura Y, Hirano K.
    • Journal Title

      Oncol. Rep.

      Volume: 28 Issue: 6 Pages: 2009-2015

    • DOI

      10.3892/or.2012.2039

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 低pH曝露による前立腺癌細胞死におけるCaspase-10の関与2014

    • Author(s)
      平田紗希、曽田翠、井口和弘、臼井茂之、北市清幸
    • Organizer
      日本薬学会東海支部例会
    • Place of Presentation
      静岡
    • Year and Date
      2014-11-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低pH曝露による前立腺がん細胞死と抗アポトーシス因子survivinの発現低下2014

    • Author(s)
      齋尾真希、曽田翠、井口和弘、臼井茂之、北市清幸
    • Organizer
      日本薬学会東海支部大会
    • Place of Presentation
      三重
    • Year and Date
      2014-07-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 前立腺癌細胞の低pH耐性化におけるプロトン感受性GPCRの関与2014

    • Author(s)
      岸上さやか、曽田翠、井口和弘、臼井茂之、北市清幸
    • Organizer
      日本薬学会東海支部大会
    • Place of Presentation
      三重
    • Year and Date
      2014-07-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低pH環境による前立腺癌細胞LNCaP細胞におけるTRAILの発現誘導2013

    • Author(s)
      霞聡子、井口和弘、早川友里、臼井茂之、平野和行
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 前立腺癌細胞株LNCaP-F10細胞のビカルタミド耐性獲得機序2012

    • Author(s)
      松本芳、井口和弘、早川友里、臼井茂之、平野和行
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] Prostate cancer LNCaP-F10 cells are resistant against cell death induced by low pH and low nutrition culture condition2011

    • Author(s)
      Kazuhiro Iguchi, Yuri Hayakawa, Kenichiro Ishii, Kaoru Matsumoto, Shigeyuki Usui, Yoshiki Sugimura, Kazuyuki Hirano
    • Organizer
      The 39th Meeting of the International Society of Oncology and BioMarkers (ISOBM2011)
    • Place of Presentation
      Firenze, Italy
    • Related Report
      2011 Research-status Report
  • [Presentation] Characterization of LNCaP-F10, a low-nutrition and low-pH-resistant LNCaP cell subline2011

    • Author(s)
      Yuri Hayakawa, Kazuhiro Iguchi, Kenichiro Ishii, Kaoru Matsumoto, Shigeyuki Usui, Yoshiki Sugimura, Kazuyuki Hirano
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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