Characterization of the low pH-resistant LNCaP cell subline
| Project/Area Number |
23791768
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | LNCaP細胞 / LNCaP-F10細胞 / 細胞外低pH / アポトーシス / survivin / caspase-10 / TNFSF10 / TRAIL / ビカルタミド / アンドロゲン受容体 / LNCaP / LNCaP-F10 |
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| Outline of Final Research Achievements |
Intratumoral region of low extracellular pH is common feature of solid tumors. The subline LNCaP-F10, of the prostate cancer cell line LNCaP, was isolated and its low pH-resistant property was examined. LNCaP-F10 cells were grown under low-pH condition, which caused apoptosis of the LNCaP cells. Significant differences in the expression of TNFSF10, BIRC5 and CASP10 were detected between LNCaP-F10 and LNCaP cells. These factors were related with the apoptosis induction by extracellular low pH.
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Report
(5 results)
Research Products
(8 results)
