Characterization of the low pH-resistant LNCaP cell subline

• Project/Area Number: 23791768
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: IGUCHI Kazuhiro 岐阜薬科大学, 薬学部, 講師 (10295545)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: LNCaP細胞 / LNCaP-F10細胞 / 細胞外低pH / アポトーシス / survivin / caspase-10 / TNFSF10 / TRAIL / ビカルタミド / アンドロゲン受容体 / LNCaP / LNCaP-F10

Outline of Final Research Achievements

Intratumoral region of low extracellular pH is common feature of solid tumors. The subline LNCaP-F10, of the prostate cancer cell line LNCaP, was isolated and its low pH-resistant property was examined. LNCaP-F10 cells were grown under low-pH condition, which caused apoptosis of the LNCaP cells. Significant differences in the expression of TNFSF10, BIRC5 and CASP10 were detected between LNCaP-F10 and LNCaP cells. These factors were related with the apoptosis induction by extracellular low pH.

Research Products

• [Journal Article] Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10. (2012)
  • Author(s): Iguchi K, Hayakawa Y, Ishii K, Matsumoto K, Usui S, Sugimura Y, Hirano K.
  • Journal Title: Oncol. Rep. Volume: 28 Issue: 6 Pages: 2009-2015
  • DOI: 10.3892/or.2012.2039
  • Peer Reviewed
• [Presentation] 低pH曝露による前立腺癌細胞死におけるCaspase-10の関与 (2014)
  • Author(s): 平田紗希、曽田翠、井口和弘、臼井茂之、北市清幸
  • Organizer: 日本薬学会東海支部例会
  • Place of Presentation: 静岡
  • Year and Date: 2014-11-09
• [Presentation] 低pH曝露による前立腺がん細胞死と抗アポトーシス因子survivinの発現低下 (2014)
  • Author(s): 齋尾真希、曽田翠、井口和弘、臼井茂之、北市清幸
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 三重
  • Year and Date: 2014-07-05
• [Presentation] 前立腺癌細胞の低pH耐性化におけるプロトン感受性GPCRの関与 (2014)
  • Author(s): 岸上さやか、曽田翠、井口和弘、臼井茂之、北市清幸
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 三重
  • Year and Date: 2014-07-05
• [Presentation] 低pH環境による前立腺癌細胞LNCaP細胞におけるTRAILの発現誘導 (2013)
  • Author(s): 霞聡子、井口和弘、早川友里、臼井茂之、平野和行
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] 前立腺癌細胞株LNCaP-F10細胞のビカルタミド耐性獲得機序 (2012)
  • Author(s): 松本芳、井口和弘、早川友里、臼井茂之、平野和行
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] Prostate cancer LNCaP-F10 cells are resistant against cell death induced by low pH and low nutrition culture condition (2011)
  • Author(s): Kazuhiro Iguchi, Yuri Hayakawa, Kenichiro Ishii, Kaoru Matsumoto, Shigeyuki Usui, Yoshiki Sugimura, Kazuyuki Hirano
  • Organizer: The 39th Meeting of the International Society of Oncology and BioMarkers (ISOBM2011)
  • Place of Presentation: Firenze, Italy
• [Presentation] Characterization of LNCaP-F10, a low-nutrition and low-pH-resistant LNCaP cell subline (2011)
  • Author(s): Yuri Hayakawa, Kazuhiro Iguchi, Kenichiro Ishii, Kaoru Matsumoto, Shigeyuki Usui, Yoshiki Sugimura, Kazuyuki Hirano
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋