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The elucidation of crystal formation mechanism through the renal tubular epithelial cell injury and mitochondria injury

Research Project

Project/Area Number 23791770
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Urology
Research InstitutionNagoya City University

Principal Investigator

HIROSE Masahito  名古屋市立大学, 大学院・医学研究科, 研究員 (70529172)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords尿路結石 / 女性ホルモン / 酸化ストレス / 細胞障害 / ミトコンドリア
Research Abstract

In this study, to elucidate about crystal formation mechanism and the low stone prevalence of the woman, I examined sex differences of crystal formation from oxidative stress and cell injury. Besides, it was aimed for development of new stone prevention by elucidating a relation with a cell injury and the oxidation stress. In the study in 2011, I studied sex differences judging from a renal tubule cell and a mitochondrial injury. I gave an oxalic acid precursor to male and female mice, and clarified a difference of the sex about a renal tubule cell and a mitochondrial injury. In stone model mouse, after oxalic acid precursor administration, mitochondria and microvilli of the renal tubular cell collapsed, aggregated in the renal tubular lumen, and crystal nuclei appeared. With the female mouse, these form changes passed slightly late in comparison with a male. In 2012, I examined osteopontin (OPN) which was a stone-related gene. The expression of OPN in the female mouse had less expression than a male before the crystal formation, and expression increasing was slow. Furthermore, a renal tubular microstructure change was slower than wild type after the treatment of the oxalic acid precursor in the examination using knockout mouse, and there were few cell disorders. And there was little quantity of crystal formation. In the female OPN knockout mouse, there was little quantity of crystals more than male. The results of the study clarified the stone formation mechanism and the importance of mitochondrial injury and oxidative stress. Possibility of the new stone prevention through the mitochondria protection was thought. And these were thought one of the new function of the female hormone.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (4 results)

All 2012 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results) Book (1 results)

  • [Journal Article] Role of osteopontin in early phase of renal crystal formation: immunohistochemical and microstructural comparisons with osteopontin knock-out mice2012

    • Author(s)
      Hirose M, Tozawa K, Okada A, Hamamoto S, Higashibata Y, Gao B, Hayashi Y, Shimizu H, Kubota Y, Yasui T, Kohri K
    • Journal Title

      Urol Res.

      Volume: 40 Issue: 2 Pages: 121-9

    • DOI

      10.1007/s00240-011-0400-z

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 尿路結石の初期形成機序に関わる尿細管細胞のオルガネラ障害と炎症について2011

    • Author(s)
      広瀬 真仁、成山 泰道、福田 勝洋、窪田 裕樹、山田 泰之
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋市
    • Related Report
      2012 Final Research Report
  • [Presentation] 尿路結石の初期形成機序に関わる尿細管細胞のオルガネラ障害と炎症について2011

    • Author(s)
      広瀬 真仁
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2011 Research-status Report
  • [Book] 泌尿器科レジデントマニュアル(監修 郡 健二郎、編集 佐々木 昌一、戸澤 啓一、丸山 哲史)2011

    • Author(s)
      広瀬 真仁
    • Publisher
      医学書院
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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