Aberrant DNA methylation of the imprinted genes in the sperm of

• Project/Area Number: 23791799
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: MIYAUCHI Naoko 東北大学, 医学(系)研究科(研究院), 技術補佐員 (60596162)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生殖補助医療（ART） / 男性精子 / DNAメチル化 / ゲノムインプリント / 生殖補助医療（ART) / ゲノムインプリンティング / メチル化合成酵素

Research Abstract

The parent-of-origin specific expression of the imprinted genes relies on DNA methylation during gametogenesis. We found aberrant DNA methylation of 22 imprinted loci in sperm from infertile men. We identified apporoximately 25%(84/337)abnormal methylation cases. We found strong correlation between methylation error and poor quality sperm(oligozoospermia),and low imprint rate and live-birth. We need carefully follow-up ART children.

Research Products

• [Journal Article] Stability of the abnormal imprinting of human induced pluripotent stem cells (2013)
  • Author(s): Hiura H, Toyoda M, Okae H, Sakurai M, Miyauchi N, Sato A, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Nishino K, Umezawa A, Arima T
  • Journal Title: BMC Genetics Volume: 14 Issue: 1 Pages: 32-32
  • DOI: 10.1186/1471-2156-14-32
  • Peer Reviewed
• [Journal Article] DNA methylation errors in imprinting disorders and assisted reproductive technologies. (2013)
  • Author(s): Chiba H, Hiura H, Okae H, Miyauchi N, Sato F, Sato A, Arima T.
  • Journal Title: Pediatrics international. Volume: 55 Issue: 5 Pages: 542-549
  • DOI: 10.1111/ped.12185
  • Peer Reviewed
• [Journal Article] High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method (2012)
  • Author(s): Hiura H, Okae H, Kobayashi H, Miyauchi N, Sato F, Sato A, Suzuki F, Nagase S, Sugawara J, Nakai K, Yaegashi N, Arima T
  • Journal Title: BMC Medical Genomics Volume: 5 Pages: 8-17
  • Peer Reviewed
• [Journal Article] Characterization of DNA methylation errors in patients with imprinting disorders conceived by assisted reproductive technologies. (2012)
  • Author(s): Hiura H, Okae H, Miyauchi N, Sato F, Sato A , Van De Pette M, John R M, Kagami M, Nakai K, Soejima H, Ogata T, Arima T.
  • Journal Title: Human Reproduction Volume: 27(8) Issue: 8 Pages: 2541-2548
  • DOI: 10.1093/humrep/des197
  • Peer Reviewed
• [Journal Article] High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method. (2012)
  • Author(s): Hiura H, Okae H, Kobayashi H, Miyauchi N, Sato F, Sato A, Suzuki F, Nagase S, Sugawara J, Nakai K, Yaegashi N, Arima T.
  • Journal Title: BMC Medical Genomics Volume: 5 Issue: 1 Pages: 8-17
  • DOI: 10.1186/1755-8794-5-8
  • Peer Reviewed
• [Journal Article] Aberrant DNA methylation of imprinted (2012)
  • Author(s): Arima T, Okae H, Hiura H, Miyauchi N at al.
  • Journal Title: INTECH Volume: 29 Pages: 183-192
  • Peer Reviewed
• [Journal Article] Assessing loss of imprint methylation in sperm from subfertile men using novel methylation PCR-Luminex analysis (2011)
  • Author(s): Sato A, Hiura H, Okae H, Miyauchi N, Abe Y, Utsunomiya T, Yaegashi N, Arima T
  • Journal Title: Fertil and Steril Volume: 95 Issue: 1 Pages: 129-134
  • DOI: 10.1016/j.fertnstert.2010.06.076
  • Peer Reviewed
• [Book] DNA METHYLATION (2012)
  • Author(s): Arima T, Okae H, Hiura H, Miyauchi N, Sato F, Sato A, Hayashi C.
  • Publisher: INTECH
• [Book] 産婦人科の実際 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子、阿部千鶴、林千賀
  • Publisher: 金原出版株式会社
• [Book] 癌と化学療法 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、宮内尚子、佐藤芙美
  • Publisher: 癌と化学療法社
• [Book] 助産雑誌 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子
  • Publisher: 医学書院
• [Book] 卵子学 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子
  • Publisher: 京都大学学術出版会