Functional analysis of estrogen receptor beta-targeting candidate gene Basonuclin1.

• Project/Area Number: 23791803
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: IHARA MOTOMASA 東北大学, 大学病院, 助教 (50403506)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 生殖 / 不妊 / 卵成熟 / SUMO化 / Bnc1 / エストロゲン受容体beta

Research Abstract

Basonuclin1 (Bnc1) gene is specifically expressed in germ cells and keratinocyte. We have identified the possibility of that Bnc1 gene expression is regulated by estrogen receptor beta. Our findings include: The localization of Bnc1 is slightly different from that of Bnc1 during early oocyte maturation; Bnc1 has another isoform and it localizes in cytoplasm; This isoform is specifically sumoylated with SUMO2/3.

Research Products

• [Journal Article] Paternal poly(ADP-ribose) metabolism modulates retention of inheritable sperm histones and early embryonic gene expression (2014)
  • Author(s): Ihara M, Meyer-Ficca ML, Leu NA, Rao S, Li F, Gregory BD, Zalenskaya IA, Schultz RM, and Meyer RG
  • Journal Title: PLoS Genet Volume: 10(5) Issue: 5 Pages: e1004317-e1004317
  • DOI: 10.1371/journal.pgen.1004317
  • Peer Reviewed
• [Journal Article] Alteration of poly(ADP-ribose) metabolism affects murine sperm nuclear architecture by impairing pericentric heterochromatin condensation (2013)
  • Author(s): Meyer-Ficca ML, Lonchar JD, Ihara M, Rader JJ, and Meyer RG
  • Journal Title: Chromosoma Volume: 122(4) Issue: 4 Pages: 319-335
  • DOI: 10.1007/s00412-013-0416-y
  • Peer Reviewed
• [Journal Article] 活性酸素 : 生殖細胞と酸化ストレス (2013)
  • Author(s): 井原基公、有馬隆博
  • Journal Title: 医歯薬出版「医学のあゆみ」 Pages: 851-855
• [Presentation] ポリ(ADP-リボシル)化反応異常はヘテロクロマチンの凝縮障害によって精子の核構造に影響する (2013)
  • Author(s): 井原基公, Mirella L. Meyer-Ficca, Julia D. Lonchar, Jessica J. Bader,八重樫伸生, Ralph G. Meyer
  • Organizer: 第61回北日本産科婦人科学会学術講演会
  • Place of Presentation: 旭川市
  • Year and Date: 2013-09-07
• [Presentation] 精子形成期のポリ(ADP-リボシル)化反応は初期胚遺伝子発現制御に関与する (2012)
  • Author(s): 井原基公, Mirella L. Meyer-Ficca, Richard M. Schultz, Jessica J. Bader,菅原準一,八重樫伸生, Ralph G. Meyer
  • Organizer: 第57回日本生殖医学会学術講演会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-11-09
• [Presentation] Basonuclin1は卵母細胞・着床前期胚におけるribosomal RNA variantの遺伝子発現制御に関与しない (2012)
  • Author(s): 井原基公・Hung Tseng・Richard M. Schultz
  • Organizer: 第52回日本哺乳動物卵子学会学術集会
  • Place of Presentation: 大田原市
  • Year and Date: 2012-05-21
• [Presentation] 精子におけるポリ(ADPリボース)化反応異常は初期胚における遺伝子発現に影響する (2011)
  • Author(s): 井原基公, Mirella L. Meyer-Ficca, Julia D. Lonchar,菅原純一,八重樫伸生, Ralph G. Meyer
  • Organizer: 第56回日本生殖医学会学術講演会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-12-09
• [Presentation] 精子におけるポリ(ADP-リボ-ス)化反応異常は初期胚における遺伝子発現に影響する (2011)
  • Author(s): 井原基公, Mirella L. Meyer-Ficca, Julia Lonchar, Kenneth J. McLaughlin,菅原準一,八重樫伸生, Richard M. Schultz, and Ralph G. Meyer
  • Organizer: 第59回北日本産科婦人科学会学術講演会
  • Place of Presentation: 秋田市
  • Year and Date: 2011-09-25