Assessment of non-invasive biomarkers for evaluating oocyte quality by proteomic analyses

• Project/Area Number: 23791827
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Nagoya University
• Principal Investigator: GOTO Maki 名古屋大学, 医学部附属病院, 助教 (90378125)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 卵胞液 / 胚発育 / プロテオーム解析 / 卵胞発育 / 顆粒膜細胞 / PCOS

Research Abstract

Non-invasive biomarkers for evaluating oocyte quality might be helpful to improve success rates of assisted reproduction technology. To identify specific biomarkers of the good quality oocytes and bioactive peptides/proteins possibly involved in folliculogenesis. We collected 24 follicular fluids (FFs) from12 patients who undergone IVF-ET at Nagoya University Hospital. Proteomic analyses were performed by LC/MS. Among all quantitatively-detected proteins, 476proteins were present in both FFs derived from oocytes of resulting in pregnancy and from degenerated oocytes. 18 proteins were present only in FFs derived from oocytes resulting in pregnancy and 9 proteins were present only in FFs derived from degenerated oocytes. The proteomic analysis using FFs revealed that FFs contain many proteins. Analysis of these peptides/proteins might be helpful to identify biomarkers implicated in folliculogenesis and oocyte quality.

Research Products

• [Journal Article] Establishment of a human nonluteinized granulosa cell line that transitions from the gonadotropin-independent to the gonadotropin-dependent status. (2012)
  • Author(s): Bayasula, Iwase A, Kiyono T, Takikawa S, Goto M, Nakamura T, Nagatomo Y, Nakahara T, Kotani T, Kobayashi H, Kondo M, Manabe S, Kikkawa F.
  • Journal Title: Endocrinology Volume: 153(6) Pages: 2851-60
  • Peer Reviewed
• [Journal Article] Establishment of a human nonluteinized granulosa cell line that transitions from the gonadotropin-independent to the gonadotropin-dependent status. (2012)
  • Author(s): Bayasula
  • Journal Title: Endocrinology Volume: 153 Issue: 6 Pages: 2851-60
  • DOI: 10.1210/en.2011-1810
  • Peer Reviewed
• [Presentation] インスリンおよび RU486 投与による PCOSモデルラット作成の試み (2012)
  • Author(s): 近藤 美佳, 岩瀬明, 杉田敦子, 中村智子, 中原辰夫, 滝川幸子, 眞鍋修一, 後藤真紀, 吉川 史隆
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸市 神戸国際展示場
  • Year and Date: 2012-04-14
• [Presentation] ヒト顆粒膜細胞における CX43 とコレステロール合成酵素の発現解析 (2012)
  • Author(s): 中村智子, 岩瀬明, 杉田敦子, 近藤 美佳,中原辰夫, 滝川幸子, 眞鍋修一, 後藤真紀, 吉川 史隆
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸市 神戸国際展示場
  • Year and Date: 2012-04-14
• [Presentation] ヒト顆粒膜細胞におけるCX43とコレステロール合成酵素の発現解析 (2012)
  • Author(s): 中村智子
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸市 神戸国際展示場
• [Presentation] インスリンおよびRU486投与によるPCOSモデルラット作成の試み (2012)
  • Author(s): 近藤美佳
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸市 神戸国際展示場
• [Presentation] 卵胞発育に関与する因子探索を目的とした卵胞液プロテオーム解析 (2011)
  • Author(s): 巴雅蘇拉 , 岩瀬明, 杉田敦子, 近藤美佳, 中村智子, 中原辰夫, 滝川幸子, 小林浩治, 眞鍋修一, 後藤真紀, 吉川史隆
  • Organizer: 第56回日本生殖医学会学術講演会
  • Place of Presentation: 横浜市 パシフィコ横浜
  • Year and Date: 2011-12-08
• [Presentation] 卵胞プロテオーム解析を用いた卵胞発育および卵成熟機構に関与する因子についての検討 (2011)
  • Author(s): 後藤真紀, 岩瀬明, 中村智子, 廣川和加奈, 中原辰夫, 小林浩治, 滝川幸子, 眞鍋修一, 吉川史隆
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪市 大阪国際会議場
  • Year and Date: 2011-08-30
• [Presentation] 卵胞液プロテオーム解析を用いた卵胞発育および卵成熟機構に関与する因子についての検 (2011)
  • Author(s): 後藤 真紀
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪国際会議場