Establishementof whole ovarian cryopreservation
| Project/Area Number |
23791831
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 生殖医学 / 卵巣凍結 |
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| Research Abstract |
The objective of this study is toestablish a technology for freezingovarian tissues using primates (cynomolgus monkeys). A technique to remove ovaries with the ovarian arteries and veins was examined in cynomolgus monkeys. Conditions of cryoprotectants used inovary freezing as well as those of slow freezing methods were studied. To evaluatethe function of frozen-thawed ovaries, viability of the cells was measured using propidium iodide (PI) and the level of estrogen in the culture solution after adding FSH was measured by ELISA.
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Report
(3 results)
Research Products
(7 results)
