Establishementof whole ovarian cryopreservation

• Project/Area Number: 23791831
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: SHIMIZU Yoshihiko 滋賀医科大学, 医学部, 助教 (50422887)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 生殖医学 / 卵巣凍結

Research Abstract

The objective of this study is toestablish a technology for freezingovarian tissues using primates (cynomolgus monkeys). A technique to remove ovaries with the ovarian arteries and veins was examined in cynomolgus monkeys. Conditions of cryoprotectants used inovary freezing as well as those of slow freezing methods were studied. To evaluatethe function of frozen-thawed ovaries, viability of the cells was measured using propidium iodide (PI) and the level of estrogen in the culture solution after adding FSH was measured by ELISA.

Research Products

• [Journal Article] Intake of vinegar beverage is associated with restoration of ovulatory function in women with polycystic ovary syndrome. (2013)
  • Author(s): Wu D, Kimura F, Takashima A, Shimizu Y, Takebayashi A, Kita N, Zhang G, Murakami T.
  • Journal Title: Tohoku J Exp Med Volume: 230 Pages: 17-23
  • NAID: 10031175415
  • Peer Reviewed
• [Journal Article] Comparison of the outcome of in vitro fertilization after laparoscopic laser ablation surgery versus laparoscopic cystectomy for endometrioma. (2013)
  • Author(s): Akie Takebayashi, Shimizu Y, Akimasa Takahashi, Akiyoshi Yamanaka, Akiko Takashima, Fuminori Kiumura, Nobuyuki Kita, Kentaro Takahashi, Takashi Murakami
  • Journal Title: Gynecology and minimally invasive therapy Volume: 2 Pages: 27-29
  • Peer Reviewed
• [Journal Article] Comparison of the outcome of in vitro fertilization after laparoscopic laser ablation surgery versus laparoscopic cystectomy for endometrioma (2013)
  • Author(s): Akie Takebayashi, Shimizu Y, Akimasa Takahashi, Akiyoshi Yamanaka, Akiko Takashima, Fuminori Kiumura, Nobuyuki Kita, Kentaro Takahashi, Takashi Murakami.
  • Journal Title: Gynecology and minimally invasive therapy Volume: 2 Issue: 1 Pages: 27-29
  • DOI: 10.1016/j.gmit.2012.12.005
  • Peer Reviewed
• [Journal Article] Successful delivery following ICSI with macrocephalic sperm head syndrome: a case report. (2012)
  • Author(s): Shimizu Y, Kiumura F, Kaku S, Izuno M, Tomita K, Thumkeo D, Murakami T.
  • Journal Title: Reprod Biomed Online Volume: 24 Pages: 603-605
  • Peer Reviewed
• [Journal Article] Successful delivery following ICSI with macrocephalic sperm head syndrome: a case report. (2012)
  • Author(s): Shimizu Y, Kiumura F, Kaku S, Izuno M, Tomita K, Thumkeo D, Murakami T.
  • Journal Title: Reprod Biomed Online. Volume: 24 Issue: 6 Pages: 603605-603605
  • DOI: 10.1016/j.rbmo.2012.02.022
  • Peer Reviewed
• [Presentation] 円錐切除後に頸管狭窄をきたした挙児希望症例の管理～狭窄の解除と子宮内膜炎の治療を行い体外受精で妊娠に至った1例 (2012)
  • Author(s): 清水良彦、木村文則、辻俊一郎、竹林明枝、高島明子、喜多伸幸、村上節
  • Organizer: 村上節円錐切除後に頸管狭窄をきたした挙児希望症例の管理～狭窄の解除と子宮内膜炎の治療を行い体外受精で妊娠に至った1例
  • Place of Presentation: 長崎
  • Year and Date: 2012-11-08
• [Presentation] 生児を得ることができた macrocephalic sperm head syndrome の 2 例 (2012)
  • Author(s): 清水良彦、伊津野美香、金子由貴、岸田和美、段亜儒、竹林明枝、高島明子、木村文則、喜多伸幸、高橋健太郎、村上節
  • Organizer: 第53回日本哺乳動物卵子学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-05-26