Induction of haploid germ cell from mammalian embryonic stem cell
| Project/Area Number |
23791859
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 生殖細胞誘導 / ES細胞 / 低酸素培養 / 再生医療 / 始原生殖細胞 / 半数体細胞 / 生殖細胞 / EpiSC / 低酸素 / Oct4 dePE / 半数体精子 / 熱刺激応答 / 高度生殖医療 |
|---|
| Research Abstract |
Although several researches for mechanism of germ cell development in mammal are proceeding to date, it almost remains unclear. Recently, it is attempted to get the germ cell or to elucidate the molecular mechanism of gametogenesis by the differentiation induction to germ cell from embryonic stem cell (ESC) in vitro. However, it is still difficult to obtain normal germ cell because almost reports showed that inducing cells were abnormal meiosis and low efficiency.
In this study, we aimed to obtain primordial germ cell under in vitro culture condition, we induce to primordial germ cell from ESC. To visualize the germ cell marker, we used Oct4-dePE GFP marker. We tried to induce by consideration of in vivo environment of embryogenesis.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Generation of Embryonic Stem Cell Lines from Immature Rabbit Ovarian Follicles2013
Author(s)
Teramura T, Sugimoto H, Frampton J, Kida Y, Nakano M, Kawakami M, Izumi H, Fukunaga N, Onodera Y, Takehara T, Fukuda K, Hosoi Y
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Journal Title
Stem Cells Dev
Volume: 22(6)
Pages: 928-38
Related Report
Peer Reviewed
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[Journal Article] Induction of Functional Mesenchymal Stem Cells in Severe Hypoxic Condition from Rabbit Embryonic Stem Cells2013
Author(s)
Teramura T, Onodera Y, Takehara T, Frampton J, Matsuoka T, Ito S, Nakagawa K, Miki Y, Hosoi Y, Hamanishi C, and Fukuda K
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Journal Title
Cell Transplantation
Volume: 22(2)
Pages: 309-29
Related Report
Peer Reviewed
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