| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Although several researches for mechanism of germ cell development in mammal are proceeding to date, it almost remains unclear. Recently, it is attempted to get the germ cell or to elucidate the molecular mechanism of gametogenesis by the differentiation induction to germ cell from embryonic stem cell (ESC) in vitro. However, it is still difficult to obtain normal germ cell because almost reports showed that inducing cells were abnormal meiosis and low efficiency.
In this study, we aimed to obtain primordial germ cell under in vitro culture condition, we induce to primordial germ cell from ESC. To visualize the germ cell marker, we used Oct4-dePE GFP marker. We tried to induce by consideration of in vivo environment of embryogenesis.
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