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Induction of haploid germ cell from mammalian embryonic stem cell

Research Project

Project/Area Number 23791859
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionKinki University

Principal Investigator

TAKEHARA Toshiyuki  近畿大学, 医学部, 助教 (60580561)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords生殖細胞誘導 / ES細胞 / 低酸素培養 / 再生医療 / 始原生殖細胞 / 半数体細胞 / 生殖細胞 / EpiSC / 低酸素 / Oct4 dePE / 半数体精子 / 熱刺激応答 / 高度生殖医療
Research Abstract

Although several researches for mechanism of germ cell development in mammal are proceeding to date, it almost remains unclear. Recently, it is attempted to get the germ cell or to elucidate the molecular mechanism of gametogenesis by the differentiation induction to germ cell from embryonic stem cell (ESC) in vitro. However, it is still difficult to obtain normal germ cell because almost reports showed that inducing cells were abnormal meiosis and low efficiency.
In this study, we aimed to obtain primordial germ cell under in vitro culture condition, we induce to primordial germ cell from ESC. To visualize the germ cell marker, we used Oct4-dePE GFP marker. We tried to induce by consideration of in vivo environment of embryogenesis.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2014 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (13 results) Remarks (3 results)

  • [Journal Article] c-Jun N-terminal kinase (JNK) mediates Rho/ROCK induced Sox9 diminution in chondrocytes2013

    • Author(s)
      Onodera Y, Teramura T, Takehara T, Fukuda K
    • Journal Title

      Acta Medica Kinki University

      Volume: 巻38 No.2

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of Embryonic Stem Cell Lines from Immature Rabbit Ovarian Follicles2013

    • Author(s)
      Teramura T, Sugimoto H, Frampton J, Kida Y, Nakano M, Kawakami M, Izumi H, Fukunaga N, Onodera Y, Takehara T, Fukuda K, Hosoi Y
    • Journal Title

      Stem Cells Dev

      Volume: 22(6) Pages: 928-38

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of Functional Mesenchymal Stem Cells in Severe Hypoxic Condition from Rabbit Embryonic Stem Cells2013

    • Author(s)
      Teramura T, Onodera Y, Takehara T, Frampton J, Matsuoka T, Ito S, Nakagawa K, Miki Y, Hosoi Y, Hamanishi C, and Fukuda K
    • Journal Title

      Cell Transplantation

      Volume: 22(2) Pages: 309-29

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cyclic compression-induced p38 activation and subsequent MMP13 expression requires Rho/ROCK activity in bovine cartilage explants2012

    • Author(s)
      Nakagawa K, Teramura T, Takehara T, Onodera Y, Hamanishi C, Akagi M, Fukuda K
    • Journal Title

      Inflamm Res

      Volume: 61(10) Pages: 1093-100

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical stimulation of cyclic tensile strain induces reduction of pluripotent related gene expressions via activation of Rho/ROCK and subsequent decreasing of AKT phosphorylation in human induced pluripotent stem cells2012

    • Author(s)
      Teramura T, Takehara T, Onodera Y, Nakagawa K, Hamanishi C, Fukuda K
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 417(2) Pages: 836-41

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells2012

    • Author(s)
      Takehara T, Teramura T, Onodera Y, Hamanishi C, Fukuda K
    • Journal Title

      Stem Cells Dev

      Volume: 21(8) Pages: 1239-49

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical stimulation of cyclic tensile strain2012

    • Author(s)
      Takeshi Teramura et al.
    • Journal Title

      Biochem Biophys Res Comm

      Volume: 417 Issue: 2 Pages: 836-841

    • DOI

      10.1016/j.bbrc.2011.12.052

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reduced Oxygen Concentration Enhances Conversion of Embryonic Stem Cells to Epiblast Stem Cells.2011

    • Author(s)
      Takehara T,
    • Journal Title

      Stem cells and Development

      Volume: Epub ahead of print Issue: 8 Pages: 1239-1249

    • DOI

      10.1089/scd.2011.0322

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ウサギ体外成熟卵子をレシピエント卵子とした核移植胚由来ES細胞の樹立2014

    • Author(s)
      竹原俊幸、木田雄太、中野美穂、杉本浩伸、寺村岳士、小野寺勇太、細井美彦、福田寛二
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都国際会議場、京都
    • Year and Date
      2014-03-04
    • Related Report
      2013 Final Research Report
  • [Presentation] INDUCTION OF DIFFERENTIATION INTO PRIMORDIAL GERM CELL FROM EPIBLAST STEM CELL DERIVED FROM MOUSE EMBRYONIC STEM CELL2013

    • Author(s)
      Toshiyuki Takehara, Takeshi Teramura, Yuta Onodera, Kanji Fukuda
    • Organizer
      11^<th> International Society for Stem Cell Research, Annual Meeting
    • Place of Presentation
      ボストン、アメリカ
    • Year and Date
      2013-06-13
    • Related Report
      2013 Final Research Report
  • [Presentation] CBP/β-cateninシグナル経路の活性化がマウス多能性幹細胞の形質転換に及ぼす影響2013

    • Author(s)
      竹原俊幸、寺村岳士、小野寺勇太、福田寛二
    • Organizer
      第12回日本再生医療学会
    • Place of Presentation
      パシフィコ横浜、横浜
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] INDUCTION OF DIFFERENTIATION INTO PRIMORDIAL GERM CELL FROM EPIBLAST STEM CELL DERIVED FROM MOUSE EMBRYONIC STEM CELL2013

    • Author(s)
      Toshiyuki Takehara
    • Organizer
      11th International Society for Stem Cell Research
    • Place of Presentation
      Boston MA, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] INDUCTION OF DIFFERENTIATION INTO PRIMORDIAL GERM CELL FROM EPIBLAST STEM CELL DERIVED FROM MOUSE EMBRYONIC STEM CELL2013

    • Author(s)
      Chisako Horikawa
    • Organizer
      The 9th Conference of the Pacific Rim Society for Fertility and Sterility
    • Place of Presentation
      Kobe Hyogo, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] INDUCTION OF DIFFERENTIATION INTO PRIMORDIAL GERM CELL FROM EPIBLAST STEM CELL DERIVED FROM MOUSE EMBRYONIC STEM CELL2013

    • Author(s)
      Toshiyuki Takehara ほか3人
    • Organizer
      ISSCR 11th
    • Place of Presentation
      Boston MA, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] マウスES細胞からの生殖細胞誘導の試み2012

    • Author(s)
      竹原俊幸、寺村岳士、小野寺勇太、浜西千秋、福田寛二
    • Organizer
      第11回日本再生医療学会
    • Place of Presentation
      パシフィコ横浜、横浜
    • Year and Date
      2012-06-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Differentiation reversibility between mouse embryonic stem cells and epiblast stem cells2012

    • Author(s)
      Toshiyuki Takehara, Takeshi Teramura, Yuta Onodera, Chiaki Hamanishi, Kanji Fukuda
    • Organizer
      10^<th> International Society for Stem Cell Research, Annual Meeting
    • Place of Presentation
      パシフィコ横浜、横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスES細胞からの生殖細胞誘導の試み2012

    • Author(s)
      竹原俊幸 他3人
    • Organizer
      第11回 日本再生医療学会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒトiPS細胞からの間葉系幹細胞の分化誘導2012

    • Author(s)
      竹原俊幸
    • Organizer
      第25回日本軟骨代謝学会
    • Place of Presentation
      ウィル愛知(名古屋市)
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒト iPS 細胞からの奇形腫を介した間葉系幹細胞の分化誘導2011

    • Author(s)
      小野寺勇太
    • Organizer
      第 26 回&#8194;日本整形外科学会基礎学術集会
    • Place of Presentation
      ベイシア文化ホール(群馬県民会館) 前橋商工会議所(前橋市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 低酸素環境下におけるマウスES細胞の網羅的解析2011

    • Author(s)
      小野寺勇太
    • Organizer
      日本プロテオーム学会2011年大会 日本ヒトプロテオーム機構第9回大会
    • Place of Presentation
      朱鷺メッセ新潟コンベンションセンター(新潟市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells2011

    • Author(s)
      竹原俊幸
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2011 Research-status Report
  • [Remarks] 近畿大学 高度先端総合医療センター 再生医療部

    • URL

      http://www.med.kindai.ac.jp/stemcell/

    • Related Report
      2013 Final Research Report
  • [Remarks] 近畿大学 高度先端総合医療センター 再生医療部

    • URL

      http://www.med.kindai.ac.jp/stemcell/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 近畿大学 高度先端総合医療センター 再生医療部

    • URL

      http://www.med.kindai.ac.jp/stemcell/

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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