| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Recently, the number of patients with endometrial cancer has been rapidly growing in Japan. In this cancer, endometrioid adneocarcinoma gradeIII have especially poor diagnosis. The present study was undertaken to examine the mechanism of the resistance to chemotherapy for endometrial cancer and "salinomycin" which is the treatment drug for cancer stem cell. We speculate that there would be resistant cancer stem cells to "salynomycin", as evidenced by MTT assay which showed incomplete growth repression for "salinomycin" to endometrial cancer cell line. Therefore, we constructed "salynomycin" resistant cell line. And we examined the expression rate of ALDH(cancer stem cell marker) by flowcytometer, and cancer stemness gene expression by Real time PCR. In our study, the expression rate of ALDH(high) was significantly observed in "salinomycin" resistant cell line. Also, we found extensively high expression of L1CAM(L1 cell adhesion molecule) by Real time PCR.
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