A novel preimplantation-specific gene Zfp371 is dispensable for early embryonic development
| Project/Area Number |
23791862
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | National Research Institute for Child Health and Development |
|---|
Principal Investigator |
OGAWA SEIJI 独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 研究員 (10573371)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 着床前期胚 / ノックアウトマウス / 胚性幹細胞 / 胚性ゲノム活性化 / Zfp371 / 転写因子 / 新規遺伝子 / 着床前期胚発生 / 胚性遺伝子活性化 / 新規遺伝 / ES細胞 |
|---|
| Research Abstract |
We have identified a novel mouse gene Zfp371 that likely encodes a SCAN zinc finger domain and is expressed specifically in preimplantation embryos and embryonic stem (ES) cells. The aim of this study was to perform a detailed analysis of its expression pattern and investigate its function in early development. This study showed that Zfp371 is expressed exclusively in preimplantation embryos and ES cells. Zfp371 knockout mice and ES cells from Zfp371 knockout blastocysts were phenotypically normal, but demonstrated numerous random chromosomal deletions. These results indicate that Zfp371 may play a role in genome stability.Our current results suggest that it may be worthwhile investigating the potential role of the human ortholog of mouse ZFP371 in the generation of embryonic chromosomal aberrations.
|
Report
(4 results)
Research Products
(14 results)
-
[Presentation] 第66回日本産科婦人科学会2014
Author(s)
小川誠司, 浜谷敏生, 阿久津英憲, 山田満稔, 菅原かな, 井上治, 戸田里実, 若松修平, 梅澤明弘, 久慈直昭, 青木大輔, 吉村泰典
Organizer
着床前期胚において特異的に発現する新規SCAN-zinc finger遺伝子Zfp371のノックアウトマウスおよびノックアウト胚性幹細胞における機能解析
Related Report
-
-
-
-
[Presentation] 第65回日本産科婦人科学会2013
Author(s)
小川誠司, 浜谷敏生, 阿久津英憲, 山田満稔, 奥村典子, 菅原かな, 井上治, 山田朝子, 梅澤明弘, 久慈直昭, 青木大輔, 吉村泰典
Organizer
着床前期胚において特異的に発現する新規SCAN-zinc finger遺伝子Zfp371の解析
Related Report
-
[Presentation] 第58回日本生殖医学会2013
Author(s)
小川誠司, 浜谷敏生, 阿久津英憲, 山田満稔, 奥村典子, 菅原かな, 井上治, 山田朝子, 上條慎太郎, 梅澤明弘, 久慈直昭, 青木大輔, 吉村泰典
Organizer
着床前期胚において特異的に発現する新規SCAN-zinc finger遺伝子Zfp371の解析
Related Report
-
-
-
-
[Presentation] 第64回日本産科婦人科学会2012
Author(s)
小川誠司, 浜谷敏生, 阿久津英憲, 山田満稔, 奥村典子, 菅原かな, 井上治, 福永朝子, 梅澤明弘, 久慈直昭, 青木大輔, 吉村泰典
Organizer
着床前期胚発生に関わる新規遺伝子Zfpiの発現解析
Related Report
-
[Presentation] 第57回日本生殖医学会2012
Author(s)
小川誠司, 浜谷敏生, 山田満稔, 阿久津英憲, 奥村典子, 菅原かな, 井上治, 福永朝子, 久慈直昭, 吉村泰典
Organizer
着床前期胚発生に関わる新規遺伝子Zfpiの発現および機能解析
Related Report
-
-
-
