Effect of in vivo over-expression of KGF by electroporativelytransfected KGF cDNA on the histology of middle ear cholesteatoma.
Project/Area Number |
23791906
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Otorhinolaryngology
|
Research Institution | Nagasaki University |
Principal Investigator |
FUKUDA Tomomi 長崎大学, 大学院・医歯薬学総合研究科, 講師 (40372776)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 真珠腫性中耳炎 / KGF / KGFR |
Research Abstract |
In our previous study, we have indicated that keratinocyte growth factor (KGF) plays an important role in middle ear cholesteatoma formation. KGF is a mesenchymal-cell-derived paracrine growth factor that specifically stimulates epithelial cell growth. In this study, we investigated the effects of over-expressed KGF in vivo. As results, a single injection of KGF expression vector coupled with electroporation induced inflammatory reaction and increased proliferative activity of epithelial cells in external auditory canal, and consequently KGF may possibly induce a cholesteatoma formation. Treatment of rats of the long-term model with SU5402, a KGFreceptor inhibitor, prevented cholesteatoma formation.
|
Report
(3 results)
Research Products
(14 results)